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Study on the local neural circuitry involved in the oro-facial sensory expression

Research Project

Project/Area Number 15591929
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOsaka University

Principal Investigator

TAKEMURA Motohide  Osaka University, Graduate School of Dentistry, Associate Professor, 大学院・歯学研究科, 助教授 (70192169)

Co-Investigator(Kenkyū-buntansha) KANG Youngnam  Osaka University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (50177755)
YOSHIDA Atsushi  Osaka University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (90201855)
YNEHARA Norifumi  Osaka University, Graduate School of Dentistry, Associate Professor, 大学院・歯学研究科, 助教授 (70124534)
MORITANI Masayuki  Osaka University, Graduate School of Dentistry, Assistant Professor, 大学院・歯学研究科, 講師 (80303981)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsTrigeminal Depressor Response / Pain / Nitric Oxide / Hypothermia / Neurotoxicity / Neuropathic Pain / Sensory Regulation / Oro-Facial / 口腔感覚 / 三叉神経感覚核 / 体性局在 / ホルマリンテスト / 顔面皮膚感覚 / GABA / c-Fos
Research Abstract

The trigeminal depressor response (TDR) is a critical vascular response following nociceptive stimulation of the ore-facial area in the clinic. There was statistical correlation between cardiovascular response and prestimulus mean arterial blood pressure (MABP) and heart rate (HR) in cats. A trigeminal depressor response was induced when the prestimulus MBP and HR were above 95 mmHg and 140 beats/min, respectively. A vasopressor response was induced when the pretimulus MBP and HR were below 95 mmHg and 140 beats/min, respectively. These results are consistent with the aspect that pain is a homeostatic emotion to keep the internal environment. Neurons immunopositive for adrenaline, noradrenaline, and γaminobutyric acid (GABA), and adrenaline ^<α2A>, GABA_A, GABA_B, and glycine receptors were distributed along the sympathoreflexive route including the rostroventral medulla and infratrigeminal nucleus. The degree of neurotoxicity induced by NO was analyzed in two temperature groupes (norm … More othermia, 37℃ ; deep hypothermia, 22℃) of cultured E16 Wistar rat cortical neurons. Hypothermia does not provide adequate protection to the neurons by acting on the mechanisms evoked by NO, so we speculate that hypothermia may not confer neuroprotection once NO is released during ischemia. A c-fos antisense oligodeoxynucleotide (ODN) was intrathecally administered at the lumbar enlargement 4 hrs before subcutaneous injection of comlete Freund's adjuvant (CFA) into the rat hind paw. The c-Fos expression in the spinal dorsal horn 2hrs after the injection of CFA was reduced by the pre-treatment with the c-fos antisense ODN. Thermal hyperalgesic behavior was also reduced for a couple of days by the pre-treatment with the c-fos antisense ODN. The c-Fos upregulation could intimately relate with the dynorphin upregulation that mediate pronociceptive function through NMDA receptors. As c-Fos make hetero-dimer with the c-Jun and binds to a cis-acting element, AP-1 site. There is evidence that estrogen receptor directly binds to estrogen receptor binding site and activate AP-1 site. We have an idea that the pain regulation in the central nervous system is different between male and female, young and old and odorant-stimulated and non-stimulated. We have unpublished data that pain related behavior (PRB) is quite different between male and female following formalin injection into the facial skin. The male PRB became female type after one-week exposure to a odorant stimulation with a essential oil of lemon. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (30 results)

All 2005 2004 2003 Other

All Journal Article (25 results) Book (2 results) Publications (3 results)

  • [Journal Article] Characteristics of the trigeminal depressor response in cats.2004

    • Author(s)
      Ohshita N
    • Journal Title

      Journal of Neuroscience Research 76

      Pages: 891-901

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Bidirectional interactions between h-channels and Na+-K+ pumps in mesencephalic trigeminal neurons.2004

    • Author(s)
      Kang Y
    • Journal Title

      Journal of Neuroscience 24・14

      Pages: 3694-3702

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 感覚刺激が誘導する三叉神経感覚核におけるc-Fos発現に対するGABA作動性薬物の刺激強度依存の効果2004

    • Author(s)
      竹村元秀
    • Journal Title

      大阪大学歯学雑誌 48・2

      Pages: 49-53

    • NAID

      10012956637

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] c-Fos発現抑制の疼痛痔痛感覚への影響:特集、痛みの分子メカニズムと臨床2004

    • Author(s)
      竹村元秀
    • Journal Title

      モレキュラーメディシン(中山書店) 41

      Pages: 703-710

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 痛みにかかわる基礎研究と治療の最前線:特集、痛みの分子メカニズムと臨床2004

    • Author(s)
      米原典史
    • Journal Title

      モレキュラーメディシン(中山書店) 41

      Pages: 637-641

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 痛みとグルタミン酸受容体:特集、痛みの分子メカニズムと臨床2004

    • Author(s)
      米原典史
    • Journal Title

      モレキュラーメディシン(中山書店) 41

      Pages: 676-684

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] NO-induced increase of excitatory amino acids level in the trigeminal nucleus caudalis of the rat with tactile hypersensitivity evoked by the loose-ligation of the inferior alveolar nerves.2004

    • Author(s)
      Fujita T
    • Journal Title

      Journal of Neurochemistry 91

      Pages: 558-567

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] NO-induced increase of excitatory amino acids level in the trigeminal nucleus caudalis of the rat with tactile hypersensitivity evoked by the loose-ligation of the inferior alveolar nerves.2004

    • Author(s)
      Fujita, T. et al.
    • Journal Title

      J.Neurochem. 91

      Pages: 558-567

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of c-Fos inhibition by antisense atrategy on nociception.2004

    • Author(s)
      Takemura, M. et al.
    • Journal Title

      Molecular Medicine 41

      Pages: 703-710

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The topics of the basic research and the forefront of the treatment concerned with the pain.2004

    • Author(s)
      Yonehara, N. et al.
    • Journal Title

      Molecular Medicine 41

      Pages: 638-641

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Glutamate receptors and pain.2004

    • Author(s)
      Yonehara, N. et al.
    • Journal Title

      Molecular Medicine 41

      Pages: 676-684

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characteristics of the trigeminal depressor response in cats.2004

    • Author(s)
      Ohshita, N. et al.
    • Journal Title

      J.Neurosci.Res. 76

      Pages: 891-901

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bidirectional interactions between h-channels and Na+-K+ pumps in mesencephalic trigeminal neurons.2004

    • Author(s)
      Kang, Y. et al.
    • Journal Title

      J.Neurosci. 24

      Pages: 3694-3702

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] c-Fos発現抑制の疼痛感覚への影響:特集、痛みの分子メカニズムと臨床2004

    • Author(s)
      竹村元秀
    • Journal Title

      モレキュラーメディシン(中山書店) 41

      Pages: 703-710

    • Related Report
      2004 Annual Research Report
  • [Journal Article] NO-induced increase of excitatory amino acids level in the trigeminal nucleus caudalis of the rat with tactile hypersensitivity evoked by the loose-ligation of the inferior alveolar nerves.2004

    • Author(s)
      米原典史
    • Journal Title

      モレキュラーメディシン(中山書店) 41

      Pages: 676-684

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of deep hypothermia on nitric oxide-induced cytotoxicity in primary cultures of cortical neurons.2003

    • Author(s)
      Varathan S
    • Journal Title

      Journal of Neuroscience Research 72

      Pages: 613-621

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quantitative analysis of the dendritic architectures of single jaw-closing and jaw-opening motoneurons in cats.2003

    • Author(s)
      Moritani M
    • Journal Title

      Experimental Brain Research 150

      Pages: 265-275

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of NMDA-nitric oxide pathways in the development of tactile hypersensitivity evoked by the loose-ligation of inferior alveolar nerves in rats.2003

    • Author(s)
      Yonehara N
    • Journal Title

      Brain Research 963・1-2

      Pages: 232-243

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An involvement of trigeminal mesencephalic neurons in regulation of occlusal vertical dimension in the guinea pig.2003

    • Author(s)
      Zhang W
    • Journal Title

      Journal of Dental Research 82

      Pages: 565-569

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synaptic organization of tooth pulp afferent terminals in the rat trigeminal sensory nuclei.2003

    • Author(s)
      Bae YC
    • Journal Title

      Journal of Comparative Neurology 463

      Pages: 13-24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of NMDA-nitric oxide pathways in the development of tactile hypersensitivity evoked by the loose-ligation of inferior alveolar nerves in rats.2003

    • Author(s)
      Yonehara, N. et al.
    • Journal Title

      Brain Res. 963

      Pages: 232-243

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of deep hypothermia on nitric oxide-induced cytotoxicity in primary cultures of cortical neurons.2003

    • Author(s)
      Varathan, S. et al.
    • Journal Title

      J.Neurosci.Res. 72

      Pages: 613-621

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quantitative analysis of the dendritic architectures of single jaw-closing and jaw-opening motoneurons in cats.2003

    • Author(s)
      Moritani, M. et al.
    • Journal Title

      Exp.Brain Res. 150

      Pages: 265-275

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An involvement of trigeminal mesencephalic neurons in regulation of occlusal vertical dimension in the guinea pig.2003

    • Author(s)
      Zhang, W. et al.
    • Journal Title

      J.Dent.Res. 82

      Pages: 565-569

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synaptic organization of tooth pulp afferent terminals in the rat trigeminal sensory nuclei.2003

    • Author(s)
      Bae, Y.C.et al.
    • Journal Title

      J.Comp.Neurol. 463

      Pages: 13-24

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 先端歯科医学の創生2005

    • Author(s)
      竹村元秀
    • Total Pages
      10
    • Publisher
      大阪大学出版会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 分子神経薬理学(臨床神経科学の基礎)第19章 Pain2004

    • Author(s)
      米原典史
    • Total Pages
      17
    • Publisher
      西村書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Varathan, S.: "Effects of deep hypothermia on nitric oxide-induced cytotoxicity in primary cultures of cortical neurons"Journal of Neuroscience Research. (In Press). 613-621 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yonehara, N.: "Involvement of NMDA-nitric oxide pathways in the development of tactile hypersensitivity evoked by the loose-ligation of inferior alveolar nerves in rats"Brain Research. 963・1-2. 232-243 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Moritani, M.: "Quantitative analysis of the dendritic architectures of single jaw-closing and jaw-opening motoneurons in cats"Experimental Brain Research. (In Press). 265-275 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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