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Functional Analysis of Dentin Matrix Protein 1 (DMP1) and Regulation Analysis of their Expression during Fracture Healing.

Research Project

Project/Area Number 15591930
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOsaka University

Principal Investigator

TOYOSAWA Satoru  Osaka University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (30243249)

Co-Investigator(Kenkyū-buntansha) KOMORI Toshihisa  Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00252677)
NAKANO Takayoshi  Osaka University, Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (30243182)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsdentin matrix protein 1 / mineralization / osteocyte / fracture healing / transgenic mouse / mRNA expression / protein localization / osteoporosis / 発現制御 / 歯 / 骨
Research Abstract

Dentin matrix protein 1 (DMP1) is one of the acidic phosphorylated extracellular matrix proteins called the SIBLING (small integrin-binding ligand, N-linked glycoproteins) family. Recent studies showed that DMP1 is expressed in the mineralized tissues and suggested that DMP1 is involved in the mineralization. We investigated the precise localization of DMP1 messenger RNA (mRNA) and protein during fracture healing. In situ hybridization demonstrated that DMP1 mRNA was strongly expressed in preosteocytes and osteocytes in the bony callus during intramembranous and endochondral ossification. However, DMP1 mRNA was not detected in osteoblasts and chondrocytes. During endochondral ossification, however, a low number of DMP1-expressing cells were identified in the cluster of hypertrophic chondrocytes. However, these DMP1-expressing cells were not hypertrophic and were likely to be osteoblast-lineage cells, which were embedded in the matrix of bone or cartilage, because type I collagen-expressing cells and invasion of capillary vessels were observed in the same area. Northern blot, in situ hybridization, and immunohistochemical analyses showed that DMP1 mRNA and protein expressions were increased until day 14 postfracture, when bony callus was formed, and then declined to a lower level during remodeling of the bony callus. Therefore, DMP1 is likely to play an important role in the mineralization of the bony callus. Secondly, we elucidated the effect of DMP1 during fracture healing in DMP1-transgenic mice. X-ray and histological analysis showed no differences during fracture healing between in wild and transgenic mice. Thirdly, we estimated the DMP1 expression and distribution in ovariectomy-induced osteoporosis rats. Immunohistochemical studies showed no change of DMP1 expression and distribution in the osteoporosis rats.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (22 results)

All 2004 Other

All Journal Article (18 results) Publications (4 results)

  • [Journal Article] Eccentric localization of osteocytes expressing enzymatic activities, protein, and mRNA signals for type 5 tartrate-resistant acid phosphatase (TRAP).2004

    • Author(s)
      中野 裕紀子
    • Journal Title

      J.Histochemn.Cytochem. 52

      Pages: 1475-1482

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of dentin matrix protein 1 (DMP1) during fracture healing.2004

    • Author(s)
      豊澤 悟
    • Journal Title

      Bone 35

      Pages: 553-561

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of dentin matrix protein 1 in tumors causing oncogenic2004

    • Author(s)
      豊澤 悟
    • Journal Title

      Modern Pathology 17

      Pages: 573-578

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression level of valosin-containing protein (VCP) as a osteomalacia.2004

    • Author(s)
      山本 慎治
    • Journal Title

      Ann.Oncol. 15

      Pages: 1432-1438

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] mRNA expression and protein localization of dentin matrix prognostic marker for gingival squamous cell carcinoma.2004

    • Author(s)
      豊澤 悟
    • Journal Title

      Bone 34

      Pages: 124-133

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 歯の分子進化2004

    • Author(s)
      豊澤 悟
    • Journal Title

      CLINICAL CALCUIM 13

      Pages: 800-807

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Eccentric localization of osteocytes expressing-------.2004

    • Author(s)
      Yukiko Nakano et al.
    • Journal Title

      J Histochem Cytochem 52

      Pages: 1475-1482

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of dentin matrix protein 1 (DMP1)-----.2004

    • Author(s)
      Satoru Toyosawa et al.
    • Journal Title

      Bone 35

      Pages: 553-561

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of dentin matrix protein 1 in tumors---.2004

    • Author(s)
      Satoru Toyosawa et al.
    • Journal Title

      Modern Pathology 17

      Pages: 573-578

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression level of valosin-containing protein-----.2004

    • Author(s)
      Shinji Yamamoto et al.
    • Journal Title

      Ann.Oncol. 15

      Pages: 1432-1438

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] mRNA expression and protein localization-----.2004

    • Author(s)
      Satoru Toyosawa et al.
    • Journal Title

      Bone 34

      Pages: 124-133

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular evolution of the tooth.2004

    • Author(s)
      Satoru Toyosawa et al.
    • Journal Title

      CLINICAL CALCIUM 13

      Pages: 800-807

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Eccentric localization of osteocytes expressing…2004

    • Author(s)
      中野 裕紀子
    • Journal Title

      J.Histochem.Cytochem. 52

      Pages: 1475-1475

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of dentin matrix protein 1(DMP1) during…2004

    • Author(s)
      豊澤 悟
    • Journal Title

      Bone 35

      Pages: 553-553

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of dentin matrix protein 1 in tumors causing2004

    • Author(s)
      豊澤 悟
    • Journal Title

      Modern Pathology 17

      Pages: 573-573

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression level of valosin-containing protein(VCP)…2004

    • Author(s)
      山本 慎治
    • Journal Title

      Ann.Oncol. 15

      Pages: 1432-1432

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Intentional replantation of an immature permanent…2004

    • Author(s)
      新谷 誠康
    • Journal Title

      Int.J.Pediar.Dent. 14

      Pages: 218-218

    • Related Report
      2004 Annual Research Report
  • [Journal Article] mRNA expression and protein localization of…2004

    • Author(s)
      豊澤 悟
    • Journal Title

      Bone 34

      Pages: 124-124

    • Related Report
      2004 Annual Research Report
  • [Publications] 豊澤 悟: "Expression of dentin matrix protein 1 in tumors…"Mod.Pathol.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 豊澤 悟: "mRNA expression and protein localization of…"Bone. 34. 124-133 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 新谷 誠康: "Identification and characterization of ameloblastin"Gene. 318. 125-136 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平 雅之: "Studies on osteogenic differentiation of rat bone"J.Oral Rehabil.. 30. 802-807 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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