| Project/Area Number |
15591941
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kuyshu Dental College |
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Principal Investigator |
GOTO Tetsuya Kuyshu Dental College, Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (70253458)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Shigeru Kuyshu Dental College, Faculty of Dentistry, Professor, 歯学部, 教授 (10118078)
KONOO Tetsurou Kuyshu Dental College, Faulty of Dentistry, Associate Professor, 歯学部, 助手 (10205437)
TANAKA Teruo Kyushu University, Graduate School of Dental Science, Professor, 歯学研究院, 教授 (60077667)
KIDO Mizuho Kyushu University, Graduate School of Dental Science, Associate Professor, 歯学研究院, 助教授 (60253457)
TOGARI Akihumi Aichi Gakuin University, Faculty of Dentistry, Professor, 歯学部, 教授 (80126325)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | substance P / synovial fibroblastic cells / neurokinin-1 / arthritis / osteoclast / RANKL / neuropeptide / sensory neuron |
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| Research Abstract |
Arthritis is often accompanied by the destruction of bone, which is caused by osteoclasts at the attachment of the synovium to bone. This study was designed to evaluate the effects of neuropeptide substance P (SP) on the formation of osteoclasts via synovial fibroblastic cells. Synovial fibroblastic cells derived from rat knee joint expressed the SP receptor; neurokinin-1 receptor (NK,-R). The addition of SP stimulated the proliferation of synovial fibroblastic cells and this effect was inhibited by SP or NK_1,-R antagonists. Increased expression of the receptor activator of nuclear factor kappaB ligand (RANKL) in synovial fibroblastic cells after the addition of SP was demonstrated by reverse transcriptase-polymerase chain reaction and immunofluorescence staining. Osteoprotegerin expression in synovial fibroblastic cells was decreased after incubation with SP. In co-cultures of synovial fibroblastic cells and rat peripheral blood monocytes, SP stimulated osteoclastogenesis. These results suggest that SP in the joint cavity may cause both hypertrophy of the synovium and induction of increased osteoclast formation through the increased expression of RANKL in the synovium.
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