| Project/Area Number |
15591950
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
FUKUSHIMA Kazuo Nihon University, School of Dentistry at Matsudo, Professor, 松戸歯学部, 教授 (20009327)
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| Co-Investigator(Kenkyū-buntansha) |
OCHIAI Tomoko (KURITA Tomoko) Nihon University, School of Dentistry at Matsudo, Lecturer(Full-Time), 松戸歯学部, 講師 (20130594)
TAKEUCHI Takeo Nihon University, School of Dentistry at Matsudo, Research Assistant(Full-Time), 松戸歯学部, 助手 (90171610)
KUWABARA Noriko (SHINOZAKI Noriko) Nihon University, School of Dentistry at Matsudo, Research Assistant(Full-Time), 松戸歯学部, 助手 (90287665)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Streptococcus mutans / Streptococcus sobrinus / Caries risk / ELISA / GTF-B / GTF-I / Monoclonal antibody / Rapid diagnosis / S.mutans / S.sobrinus / モノクローナル |
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| Research Abstract |
Streptococcus mutans and Streptococcus sobrinus secrete glucosyltransferase(GTF)-B and GTF-I, which are considered to be the important factors of cariogenic biofilm formation. In order to deve-lope the rapid immuno-chromato kits for caries-risk estimation using anti-GTF monoclonal antibodies, we done the improvment of the GTF-B detection system consists of sandwich enzyme-linked immunosorbent assay(ELISA) using a mouse monoclonal and a rabbit polyclonal antibodies against GTF-B. The recombinant (r) GTF-B was highly purified from culture fluids of KSB8 transformant and used forcariblation of the developed ELISA system. This assay system could quantify with accuracy small amounts (1-100 ng)of the purified rGTF-B. In addition, we analyzed GTF-B productivity of S. mutans by this ELISA systemand indicate d that this system is a good way as examination of the GTF-B level in culture samples, and suggested that this may be useful tool for quantification of the amount of GTF-B in clinical plaque samples. Furthermore、we developed the GTF-I detection system consists of sandwich enzyme-linked immunosorbent assay(ELISA) using a mouse monoclonal and a rabbit polyclonal antibodies against GTF-I.
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