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Elucidation of Mechanisms in Fimbrial Morphogenesis in Periodontopathogens

Research Project

Project/Area Number 15591957
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionAichi-Gakuin University

Principal Investigator

YOSHIMURA Fuminobu  Aichi-Gakuin University, School of Dentistry, Microbiology, professor, 歯学部, 教授 (50001962)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Yukitaka  Aichi-Gakuin University, School of Dentistry, Microbiology, associate professor, 歯学部, 助教授 (60239506)
NISHIKAWA Kiyoshi  Aichi-Gakuin University, School of Dentistry, Microbiology, instructor, 歯学部, 講師 (50340146)
NISHIYAMA So-ichiro  Aichi-Gakuin University, School of Dentistry, Microbiology, instructor, 歯学部, 講師 (30343651)
SATO Keiko  Aichi-Gakuin University, School of Dentistry, Microbiology, assistant, 歯学部, 助手 (70410579)
永野 恵司  愛知学院大学, 歯学部, 助手 (60367620)
長谷川 義明  愛知学院大学, 歯学部, 助手
池田 健  愛知学院大学, 歯学部, 講師 (80241131)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsperiodontopathogen / fimbriae / FimA fimbriae / Mfa fimbriae / fimA gene / genotype / morphogenesis / anaerobe / 歯周病原細菌 / ゲノタイプ / MFA線毛 / fimA関連遺伝子 / トランスポゾン / 二成分調節系 / 環境変化 / 温度感受性
Research Abstract

1.Fimbriae in Porphyromonas gingivalis, a periodontopathogen
P.gingivalis seems to carry three different types of fimbriae which are composed of 40-kDa FimA,75-kDa Mfa, and 53-kDa protein, respectively, as a major subunit.
2.Distribution of the three fimbriae in P.gingivalis
Strain W83 seems to carry none of them. Although it has the fimA (genotype IV) gene, it does not seem to express FimA fimbriae. Because the mfa gene is disrupted in the chromosome, it does not produce Mfa fimbriae. Two fimbriae, FimA and Mfa, are produced in strain ATCC 33277. Some of the P.gingivalis strains including 381 appear to have the three fimbriae.
3.Variation of fimA fimbriae
There are six genotypes in the fimA gene, thus far reported. We have purified fimbriae from genotype I,Ib, and II strains. They have a plenty of fimbriae for purification. However, we do not know whether all the genotype strains carry a plenty of fimbraie on the cell surface.
4.Different extraction methods for fimbriae from strains with different fimA genotypes
Genotype I fimbraie can be selectively extracted by a simple washing of bacterial cells with a mild buffer. However, other genotype fimbraie of Ib and II could not be extracted by such washing. They can be solubilized through the cell breakage by a French Press. These results might imply that they have fibers with very different lengths. This assumption that genotype I fimbriae are long, but other genotypes of fimbraie are too short to be sheared off from the cell surfaces could be verified.
5.Regulation in fimbrial morphogenesis
The genes of fimXY appear to play an important role in expression of fimA protein in fimbrial morphogenesis. The product of the fimR gene, a positive regulator, is discovered to bind to the region upstream from fimXY for production of the fimbrial protein.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (39 results)

All 2006 2005 2004 2003 Other

All Journal Article (36 results) Publications (3 results)

  • [Journal Article] Arginine Inhibits Na^+-Driven Flageller Motors of Alkaliphilic Bacillus.2006

    • Author(s)
      Atsumi, T, et al.
    • Journal Title

      FEMS Microbiol. Lett. 256

      Pages: 224-228

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of Various Growth Conditions in a Chemostat on the Expression of Virulence Factors in Porphyromonas gingivalis2006

    • Author(s)
      Masuda, T, et al.
    • Journal Title

      Apl. Environ. Microbiol. 未定(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Arginine Inhibits Na^+-Driven Flagellar Motors of Alkaliphilic Bacillus2006

    • Author(s)
      Atsumi, T., et al.
    • Journal Title

      FEMS Microbiol.Lett. 256

      Pages: 224-228

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of Various Growth Conditions in a Chemostat on Virulence Factors in Porphyromonas gingivalis2006

    • Author(s)
      Masuda, T. et al.
    • Journal Title

      Appl.Environ.Microbiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Arginine Inhibits Na^+-Driven Flagellar Motors of Alkaliphilic Bacillus.2006

    • Author(s)
      Atsumi, T.et al.
    • Journal Title

      FEMS Microbiol.Lett. 256

      Pages: 224-228

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of Various Growth Conditions in a Chemostat on the Expression of Virulence Factors in Porphyromonas gingivalis2006

    • Author(s)
      Masuda, T.et al.
    • Journal Title

      Appl.Environ.Microbiol. (未定)(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Trimeric Structure of Major Outer Membrane Proteins Homologous to OmpA in Porphyromonas gingivalis2005

    • Author(s)
      Nagano, K, et al.
    • Journal Title

      J. Bacteriol. 187・3

      Pages: 902-911

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Short Fimbriae of Porphyromonas gingivalis and Their Rol in Coadhesion with Stereptococus gordonii2005

    • Author(s)
      Park, Y, et al.
    • Journal Title

      Infect. Immun. 73・7

      Pages: 3983-3989

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sixteen Homologs of the Mex-TYpe Multidrug Resistance Efflux Pump in Becteroides fragilis2005

    • Author(s)
      Ueda, O, et al.
    • Journal Title

      Antimicrob. Agents Chemother. 49・7

      Pages: 2807-2815

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Major Outer Membrane Proteins from Porphyromonas gingivalis : Strain Variation, Distribution. and Clinical Significance in Periradicular Lesions2005

    • Author(s)
      Imai, M, et al.
    • Journal Title

      Eur. J. Oral Sci. 113・5

      Pages: 391-399

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 歯周病関連細菌Porphyromonas gingivalisの主要外膜蛋白質Pgm6/7は外膜構造の安定性に関与する2005

    • Author(s)
      岩味潤, 他
    • Journal Title

      日本嫌気性菌感染研究 35

      Pages: 82-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Trimeric Structure of Major Outer Membrane Proteins Homologous to OmpA in Porphyromonas gingivslis2005

    • Author(s)
      Nagano, K., et al.
    • Journal Title

      J.Bacteriol. 187-3

      Pages: 902-911

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Short Fimbriae of Porphyromonas gingivalis and Their Role in Coadhesion with Streptococcus gordonii2005

    • Author(s)
      Park, Y., et al.
    • Journal Title

      Infect.Immun. 73-7

      Pages: 3983-3989

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sixteen Homologs of the Mex-Type Muttidrug Resistance Efflux Pump in Bacteroides fragilis2005

    • Author(s)
      Ueda, O. et al.
    • Journal Title

      Antimicrob.Agents Chemother. 49-7

      Pages: 2807-2815

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Major Outer Membrane Proteins from Porphyromonas gingivalis : Strain Variation, Distribution, and Clinical Significance in Periradicular Lesions2005

    • Author(s)
      Imai, M., et al.
    • Journal Title

      Eur.J.Oral Sci. 113

      Pages: 391-399

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Short Fimbriae of Porphyromonas gingivalis and Their Role in Coadhesion with Streptococcus gordonii2005

    • Author(s)
      Park, Y.et al.
    • Journal Title

      Infect.Immun. 73・7

      Pages: 3983-3989

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sixteen Homologs of the Mex-Type Multidrug Resistance Efflux Pump in Bacteroides fragilis2005

    • Author(s)
      Ueda, O.et al.
    • Journal Title

      Antimicrob.Agents Chemother. 49・7

      Pages: 2807-2815

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Major Outer Membrane Proteins from Porphyromonas gingivalis : Strain Variation, Distribution, and Clinical Significance in Periradicular Lesions2005

    • Author(s)
      Imai, M.et al.
    • Journal Title

      Eur.J.Oral Sci. 113・5

      Pages: 391-399

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 歯周病関連細菌Porphyromonas gingivalisの主要外膜蛋白質Pgm6/7は外膜構造の安定性に関与する2005

    • Author(s)
      岩味潤 他
    • Journal Title

      日本嫌気性感染症研究 35

      Pages: 82-86

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Trimeric structure of major outer membrane proteins homologous to OmpA in Porphyromonas gingivalis2005

    • Author(s)
      Nagano, K. et al.
    • Journal Title

      J.Bacteriol. 187(3)

      Pages: 902-911

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The short fimbriae of Porphyromonas gingivalis and their role in coadhesion with Streptococcus gordonii2005

    • Author(s)
      Park, Y. et al.
    • Journal Title

      Infect.Immun. (未定)(In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Sixteen homologs of the Mex-type multi-drug resistance(MDR) efflux pump in Bacteroides fragilis2005

    • Author(s)
      Ueda, O. et al.
    • Journal Title

      Antimicrob.Agent Chemother. (未定)(In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of Various Culture Environments on Expression of Major Outer Membrane Proteins from Porphyromonas gingivalits2004

    • Author(s)
      Murakami, Y, et al.
    • Journal Title

      FEMS Microbiol. Lett. 230

      Pages: 159-165

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of Major Virulence Facters in Porphyromonas gingivalis under Various Culture Temperatures Using Specific Antibodies2004

    • Author(s)
      Murakami, Y, et al.
    • Journal Title

      Microbiol. Immunol. 48・8

      Pages: 561-569

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A Regulation Cascade Controls Expression of Porphyromonas gingivalis Fimbriae via the FimR Response Regulator2004

    • Author(s)
      Nishikawa, K, et al.
    • Journal Title

      Mol. Microbio1. 54・2

      Pages: 546-560

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Localization of Porphyromonas gingivalis-Carrying Fimbria in situ in Human Periodontal Pockets2004

    • Author(s)
      Noiri, Y, et al.
    • Journal Title

      J. Dent. Res. 83・12

      Pages: 941-945

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of Various Culture Environments on Expression of Major Outer Membrane Proteins from Porphyromonas gingivalis2004

    • Author(s)
      Murakami, Y. et al.
    • Journal Title

      FEMS Microbiol.Lett. 230

      Pages: 159-165

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A Regulation Cascade Controls Expression of Porphyromonas gingivalis Fimbriae via the FimR Response Regulator2004

    • Author(s)
      Nishikawa, K., et al.
    • Journal Title

      Mol.Microbiol. 54-2

      Pages: 546-560

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of Major Virulence Factors in Porphyromonas gingivalis under Various Culture Temperatures Using Specific Antibodies2004

    • Author(s)
      Murakami, Y. et al.
    • Journal Title

      Microbiol.Immunol. 48-8

      Pages: 561-569

    • NAID

      10013440275

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Localization of Porphyromonas gingivalis-Carrying Fimbriae in situ in Human Periodontal Pockets2004

    • Author(s)
      Noiri, Y. et al.
    • Journal Title

      J.Dent.Res. 83-12

      Pages: 941-945

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of major virulence factors in Porphyromonas gingivalis under various culture temperatures using specific antibodies2004

    • Author(s)
      Murakami, Y. et al.
    • Journal Title

      Microbiol.Immunol. 48(8)

      Pages: 561-569

    • NAID

      10013440275

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A regulation cascade controls expression of Porphyromonas gingivalis fimbriae via the FimR response regulator2004

    • Author(s)
      Nishikawa, K. et al.
    • Journal Title

      Mol.Microbiol. 54(2)

      Pages: 546-560

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Localization of Porphyromonas gingivalis-carrying fimbriae in situ in human Periodontal pockets2004

    • Author(s)
      Noiri, Y. et al.
    • Journal Title

      J.Dent.Res. 83(12)

      Pages: 941-945

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 歯周病関連細菌Porphyromonas gingivalisのジンジパイン産生量の比較・連続培養による増殖速度を変化させた場合2003

    • Author(s)
      益田隆志, 他
    • Journal Title

      日本嫌気性菌感染症研究 33

      Pages: 90-101

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A Novel Type of Two-component Regulatory System Affecting Gingipains in Porphyromonas gingivalis2003

    • Author(s)
      Hasegawa, Y, et al.
    • Journal Title

      Microbiol. Immunol. 47・1

      Pages: 849-858

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A Novel Type of Two-Component Regulatory System Affecting Gingipains in Porphyromonas gingivalis2003

    • Author(s)
      Hasegawa, et al.
    • Journal Title

      Microbiol.Immunol. 47-11

      Pages: 847-858

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] 益田 隆志 他: "歯周病関連細菌Porphyromonas gingivalisのジンジパイン産生量の比較 -連続培養による増殖速度を変化させた場合-"日本嫌気性菌感染症研究. 33巻. 96-101 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hasegawa, Y.et al.: "A novel type of two-component regulatory system affecting gingipains in Porphyromonas gingivalis"Microbiol.Immunol.. 47(1). 849-858 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Murakami, Y.et al.: "Effects of various culture environments on expression of major outer membrane proteins from Porphyromonas gingivalis"FEMS Microbiol.Lett.. 230. 159-165 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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