Role of DEC1 and MTf in osteogenic and chondrogenic differentiation of mesenchymal stem cells

• Project/Area Number: 15591966
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: HIROSHIMA UNIVERSITY
• Principal Investigator: KAWAMOTO Takeshi Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (50224861)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: DEC1 / DEC2 / MTf / mesenchymal stem cell / osteogenesis / chondorogenesis / differentiation / circadian rhythm / Mesenchymal stem cell / Circadian rhythm / Osteogenesis / Chondrogenesis

Research Abstract

Since MSC has multilineage potential, we investigated the roles of DEC1 in osteogenic and adipogenic differentiation of human bone marrow-derived MSC. After osteogenic induction of MSC in medium containing dexamethasone, beta-glycerophosphate, and ascorbic acid, DEC1 expression gradually increased from day 5 to day 14 ; while expression levels of DEC1 mRNA markedly decreased on days 3 and 7 after adipogenic induction. Under osteogenic conditions, infection with DEC1-expressing adenovirus enhanced the induction of osteopontin and alkaline phosphatase, and matrix calcification. Even in the absence of the osteogenic induction medium, DEC1 overexpression increased osteopontin and alkaline phosphatase expression, although it did not induce calcification. In contrast, forced expression of DEC1 suppressed adipogenic differentiation of MSC. These findings indicate that increased DEC1 expression facilitates osteogenic differentiation of mesenchymal stem cells, and inhibits the onset of adipogenic differentiation.

Research Products

• [Journal Article] A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation. (2004)
  • Author(s): Kawamoto, T., Noshiro, M., Sato, S., Maemura, K., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 313 Pages: 117-124
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Dec1 and Dec2 expression is disrupted in the suprachiasmatic nuclei of Clock mutant mice. (2004)
  • Author(s): Butler, B.P., Honma, S., Fukumoto, T., Kawamoto, T., et al.
  • Journal Title: J.Biol.Rhythms. 19 Pages: 126-134
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rhythmic expression of DEC1 and DEC2 in peripheral tissues : DEC2 is a potent suppressor for hepatic cytochrome P450s opposing DBP. (2004)
  • Author(s): Noshiro, M., Kawamoto, T., et al.
  • Journal Title: Genes Cells 9 Pages: 317-329
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of Dec2 - a basic helix-loop-helix transcription factor- gene is regulated by a molecular clock system. (2004)
  • Author(s): Hamaguchi, H, Fujimoto, K., Kawamoto, T., et al.
  • Journal Title: Biochem.J. 382 Pages: 43-50
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional analysis of the bHLH transcription factor DEC1 in circadian regulation : Interaction with BMAL1. (2004)
  • Author(s): Sato, F., Kawamoto, T., et al.
  • Journal Title: Eur.J.Biochem. 271 Pages: 4409-4419
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Collocational Analysis of Life Science English (1) --- Lists of common collocates of possibility, probability, implication, involvement, absence, presence, evidence --- (2004)
  • Author(s): Kawamoto, T., Fujita, N., et al.
  • Journal Title: Studia Humana et Naturalia 38 Pages: 19-53
  • NAID: 110004621860
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation. (2004)
  • Author(s): Kawamoto, T., Noshiro, M., Sato, F., Maemura, K., Takeda, N., Nagai, R., Iwata, T., Fujimoto, K., Furukawa, M., Miyazaki, K., Honma, S., Honma, K., Kato, Y.
  • Journal Title: Biochem.Biophys.Res.Commun. 313 Pages: 117-124
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Rhythmic Expression of DEC1 and DEC2 in Peripheral Tissues : DEC2 is a Potent Suppressor for Hepatic Cytochrome P450s Opposing DBP. (2004)
  • Author(s): Noshiro, M., Kawamoto, T., Furukawa, M., Fujimoto, K., Yoshida, Y., Sasabe, E., Tsutsumi, S., Hamada, T., Honma, S., Honma, K., Kato, Y.
  • Journal Title: Genes Cells 9 Pages: 317-329
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dec1 and Dec2 Expression Is Disrupted in the Suprachiasmatic Nuclei of Clock Mutant Mice. (2004)
  • Author(s): Butler, M.P., Honma, S., Fukumoto, T., Kawamoto, T., Fujimoto, K., Noshiro, M., Kato, Y., Honma, K.
  • Journal Title: J.Biol.Rhythms 19 Pages: 126-134
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of Dec2 - a basic helix-loop-helix transcription factor- gene is regulated by a molecular clock system. (2004)
  • Author(s): Hamaguchi, H., Fujimoto, K., Kawamoto, T., Noshiro, M., Maemura, K., Takeda, N., Nagai, R., Furukawa, M., Honma, S., Honma, K., Kurihara, H., Kato, Y
  • Journal Title: Biochem.J. 382 Pages: 43-50
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional analysis of the bHLH transcription factor DEC1 in circadian regulation : Interaction with BMAL 1. (2004)
  • Author(s): Sato, F., Kawamoto, T., Fujimoto, K., Noshiro, M., Honda, K., Honma, S., Honma, K., Kato, Y.
  • Journal Title: Euro.J.Biochem. 271 Pages: 4409-4419
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Collocational Analysis of Life Science English (1) --- Lists of common collocates of possibility, probability, implication, involvement, absence, presence, evidence --- (2004)
  • Author(s): Kawamoto, T., Fujita, N., Kaneko, S., Morren, B., Ohtake, H.
  • Journal Title: Studia Humana et Naturalia 38 Pages: 19-53
  • NAID: 110004621860
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The role of the nuclear receptor CAR as a coordinate regulator of hepatic gene expression in defense against chemical toxicity. (2003)
  • Author(s): Yamamoto, Y., Kawamoto, T., Negishi, M.
  • Journal Title: Arch.Biochem.Biophys. 409 Pages: 207-211
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of the nuclear receptor CAR : HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to henobarbital. (2003)
  • Author(s): Yoshinari, K., Kobayashi, K., Moore, R., Kawamoto, T., et al.
  • Journal Title: FEBS Lett. 548 Pages: 17-20
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of the nuclear receptor CAR : HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital. (2003)
  • Author(s): Yoshinari, K., Kobayashi, K., Moore, R., Kawamoto, T., Negishi, M.
  • Journal Title: FEBS Lett. 548 Pages: 17-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamamoto, Y., Kawamoto, T., Negishi, M.: "The role of the nuclear receptor CAR as a coordinate regulator of hepatic gene expression in defense against chemical toxicity"Arch.Biochem.Biophys.. 409. 207-211 (2003)
• [Publications] Yoshinari, K., Kobayashi, K., Moore, R., Kawamoto, T., et al.: "Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to henobarbital"FEBS Lett.. 548. 17-20 (2003)
• [Publications] Kawamoto, T., Noshiro, M., Sato, S., Maemura, K., et al.: "A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation"Biochem.Biophys.Res.Commun.. 313. 117-124 (2004)
• [Publications] Butler, B.P., Honma, S., Fukumoto, T., Kawamoto, T., et al.: "Dec1 and Dec2 expression is disrupted in the suprachiasmatic nuclei of Clock mutant mice"J.Biol.Rhythms. (in press). (2004)