| Project/Area Number |
15591989
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | The Nippon Dental University College at Niigata |
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Principal Investigator |
ISEMURA Satoko Nippon Dental University, College at Niigata, Prof., 教授 (10112963)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Sachiyo Nippon Dental University, College at Niigata, Assistant, 助手 (30320881)
SATO Ritsuko Nippon Dental University, College at Niigata, Senior Assistant Prof., 講師 (50178787)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | saliva / tooth germ / proline-rich protein / P-B / cold shock vector / protein expression / chimeric DNA / ヒト唾液 / ウシ歯胚 / キメラ構造 / ウシ / ウシゲノムDNA / 大腸菌DNA / ヒト / 定量PCR |
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| Research Abstract |
Tissue distribution of proline-rich protein P-B was studied by RT-PCR using various bovine tissues. The results indicate that heart, submaxillary gland, tooth germ and kidney are the major sites of P-B expression. The presence of P-B mRNA in tissue other than salivary gland may suggest a fundamental physiological role of this protein.
A clone obtained from tooth germ mRNA by RT-PCR had a chimeric structure composed of two parts, one corresponding to P-B mRNA and the other to the partial sequence of E.coli genomic DNA. Whether this chimeric structure has effects on the function or expression of P-B is not known.
Production of P-B by E.coli using pCold shock vector I was tested. Although small amount of P-B production was detected, the yield is not enough for function analysis.
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