| Project/Area Number |
15591999
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | NAGASAKI UNIVERSITY |
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Principal Investigator |
HOTOKEZAKA Yuka NAGASAKI UNIVERSITY, HOSPITAL OF MEDICINE AND DENTISTRY, INSTRUCTOR, 医学部・歯学部附属病院, 助手 (10244089)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Ikuo NAGASAKI UNIVERSITY, HOSPITAL OF MEDICINE AND DENTISTRY, INSTRUCTOR, 医学部・歯学部附属病院, 助手 (80295089)
SUMI Tadateru NAGASAKI UNIVERSITY, GRADUATE SCHOOL OF BIOMEDICAL SCIENCIES, INSTRUCTOR, 大学院・医歯薬学総合研究科, 助手 (80284701)
TASHIRO Shigeki NAGASAKI UNIVERSITY, GRADUATE SCHOOL OF BIOMEDICAL SCIENCIES, INSTRUCTOR, 大学院・医歯薬学総合研究科, 助手 (20300882)
NAKAMURA Takashi NAGASAKI UNIVERSITY, GRADUATE SCHOOL OF BIOMEDICAL SCIENCIES, PROFESSOR, 大学院・医歯薬学総合研究科, 教授 (30172406)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | p28^<nrp> / ionizing radiation / repair DNA / apoptosis / p54^<nrb> |
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| Research Abstract |
It is well known that the cell arrest its cell cycle in order to repair damaged DNA in response to ionizing radiation. However, the cell may activate one or more of the apoptosis pathway to eliminate cells harboring severely damaged DNA. Here we report the identification of a new protein factor p28^<nrp> (28 kDa) that was induced in the nucleus in response to ionizing radiation. Westernblotting using p54^<nrb> peptide antibody demonstrated that the p28^<nrp> was an N-terminal part of the p54^<nrb> (nuclear RNA-binding protein). We showed that the p54^<nrb> was cleaved and an N-terminal portion (p28^<nrp>) entered into nucleus in response to ionizing radiation. It is shown recently that the polypyrimidine tract-binding protein-associated splicing factor (PSF)・p54^<nrb> complex cooperates with Ku protein to reconstitutes efficient double-strand break rejoining (J.Biol Chem,2005). Thus, it is suggested that the p28^<nrp> plays a role in repair or apoptosis to ionizing radiation.
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