| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Yoko Nihon University, School of Dentistry, Assistant, 歯学部, 副手 (00239922)
OTSUKA Kichibee Nihon University, School of Dentistry, Professor, 歯学部, 教授 (50059995)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
We examined whether fibronectin produced by periodontal tissue-derived fibroblasts participates in the initiation of periodontitis by its chemotactic activity to a gingival epithelial cell line. The extracellular matrix component, fibronectin, laminin-8/9, and laminin-2/4 secreted by periodontal ligament fibroblasts induced chemotaxis of gingival epithelial cells. To examine RNA integrity during tissue transportation, various conditions were tested. RNA was stable in room temperature for at least 24 h, however, the gene expression profile was stable when the tissue was stored at 4 degrees C examined by real-time PCR. For the accurate diagnosis of periodontal disease, we examined the profiles of cytokine in gingival crevicular fluid using Cytokine Antibody Array. We have found ten novel cytokine, growth factor and its related protein (GRO, Ang, IGFBP-3, GDNF, PARC, OSM, FGF-4, IL-16, PlGF) in gingival crevicular fluid from periodontitis-affected sites. The results suggested that the initiation and progression of periodontal disease may be induced by not only inflammatory cytokine but by various growth factors and its related protein. In the last experiment, we detected that PDGF-B transfected sis-NIH3T3 fibroblasts' spontaneous migration is inhibited by cellular fibronectin.
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