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Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures.

Research Project

Project/Area Number 15592024
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionKagoshima University

Principal Investigator

TOKUDA Masayuki  Kagoshima University, University Hospital, Faculty of Medicine and Dentistry, Assistant Professor, 医学部・歯学部附属病院, 講師 (20253891)

Co-Investigator(Kenkyū-buntansha) TORII Mitsuo  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (30116066)
NAGAOKA Shigetaka  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (10155913)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsDental pulp cells / Temperature-sensitive receptor / VR-1 / Inflammatory cytokines / TNF-α / IL-6 / MAPK / apoptosis / TNF-a
Research Abstract

We examined whether human dental pulp fibroblasts(PF-10) have vanilloid receptor subtype 1(VR1) and its relationship with interleukin(IL)-6 expressions. Results of RT-PCR, Western blotting and immunocytochemical analysis indicate VR1 expression on PF-10 cells. As shown by ELISA, VR1 antagonist, capsazepine inhibited capsaicin-induced IL-6 production which is implicated in pulp inflammation. Furthermore, we investigated the activities of mitogen-activated protein kinases(MAPKs) in capsaicin induced IL-6 production. The phosphorylation of p38 MAPK and c-Jun NH_2-terminal kinase(JNK) were detected after capsaicin stimulation. p38 MAPK is involved in capsaicin-induced IL-6 production, as shown by the use of specific inhibitors of this kinase. The result of EMSA showed capsaicin inhibited tumor necrosis factor-alpha(TNF-α)-induced nuclear factor-kappa B(NF-κB) activations. These results suggest that the activation of VR1 may play an important role in dental pulp inflammation.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (5 results)

All 2004 Other

All Journal Article (4 results) Publications (1 results)

  • [Journal Article] Substance P enhances expression of lipopolysaccharide-induced inflammatory factors in dental pulp cells2004

    • Author(s)
      Tokuda M, Miyamoto R, Nagaoka S, Torii M
    • Journal Title

      Journal of Endodontics 30

      Pages: 770-774

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures.

    • Author(s)
      R.Miyamoto, M.Tokuda, T.Sakuta, S.Nagaoka, M.Tori
    • Journal Title

      Journal of Endodontics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures.

    • Author(s)
      Miyamoto R, Tokuda M, Sakuta T, Nagaoka S, Tori M
    • Journal Title

      Journal of Endodontics (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures.

    • Author(s)
      R.Miyamoto, M.Tokuda, T.Sakuta, S.Nagaoka, M.Torii
    • Journal Title

      Journal of Endodontics (in press)

    • Related Report
      2004 Annual Research Report
  • [Publications] Tokuda M, Miyamoto R, Nagaoka S, Torii M: "Substance P enhances expression of lipopolysaccharide-induced inflammatory factors in dental pulp cells"Journal of Endodontics. (in press).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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