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Study of regeneration therapy use of stem cells in dental pulp tissues

Research Project

Project/Area Number 15592028
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionMeikai University

Principal Investigator

YOKOSE Satosi  Meikai University, School of Dentistry, Associate professor, 歯学部, 助教授 (90245803)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsPlatelet-derived growth factor / Dental pulp cells / Odontoblast / Regeneration / cell culture / PDGiF / Reparative dentinogenesis / 細胞分化 / 神経細胞 / MSX-1 / 細胞増殖
Research Abstract

It is well known that platelet-derived growth factor (PDGF) plays important roles in the process of embryogenesis and regeneration in connective tissues. Patch mutation mice display growth retardation and deficiencies in dental ectomesenchyme. This indicates that dental pulp tissue may be the target for PDGF, however, much about the mode of action of PDGF on odontoblast differentiation remain unclear. The purpose of this study is to examine the effects of each PDGF dimer (PDGF AA, AB or BB) on cell proliferation, migration and differentiation of dental pulp cells, and to obtain fundamental information concerned with clinical application of PDGF to vital pulp therapy. Dental pulp cell isolated from rat lower incisors were continuously treated with each dimer for 12 days. PDGF AB and BB stimulated cell proliferation and elicited chemotaxis dose-dependently. PDGF AA had no effects on either cell proliferation or cell migration. Although the dental pulp cells formed mineralized nodule containing osteocalcin in both control and PDGF AA treatment groups on day 12, the nodule formation was greatly inhibited in the cells treated with each PDGF AB or BB. The cells treated with each PDGF AB or BB showed the suppression of mRNA of dentin sialoprotein (DSP) which is a specific protein expressed in mature odontoblast. The cells treated with PDGF AA exhibited the same level of DSP mRNA expression as that of control group. Alkaline phosphatase (ALP) activity in the cells was inhibited by either PDGF AB or BB throughout culture period, but PDGF AA had no effects on ALP activity in the cells. Immunohistochemical analysis of PDGF receptors (α and β) demonstrated that the cells in every culture expressed the both receptors throughout culture period. These results indicate that PDGF exerts diverse effects on dentinogenesis depending on the dimers. These findings can explain partially the in vivo action of PDGF in regeneration of damaged dental pulp.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (3 results)

All 2005 Other

All Journal Article (2 results) Publications (1 results)

  • [Journal Article] 歯髄培養細胞における血小板由来増殖因子の作用について2005

    • Author(s)
      松岡哲明, 横瀬敏志
    • Journal Title

      日本歯科保存学会雑誌 48・2(印刷中)

    • NAID

      10015686669

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] In vitro effects of platelet-derived growth factor on dentinogenesis in dental pulp cells2005

    • Author(s)
      Tetsuaki Matsuoka, Satoshi Yokose
    • Journal Title

      The Japanese Journal of Conservative Dentistry 48(2) (in press)

    • NAID

      10015686669

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] S.Yokose, H.Kadokura, N.Tajima et al.: "Platelet-derived growth factor exerts disparate effects on odontoblast differentiation depending on the dimers in rat dental pulp cells."Cell and Tissue Research. 315・3. 375-384 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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