| Project/Area Number |
15592031
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
OGISO Bunnai Nihon University, School of Dentistry, Associate Professor, 歯学部, 助教授 (70147643)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEICHI Osamu Nihon University, School of Dentistry, Lecturer, 歯学部, 講師 (10277460)
HAYASHI Makoto Nihon University, School of Dentistry, Assistant, 歯学部, 助手 (00301557)
MATSUZAKA Ken-ichi Tokyo Dental College, Associate Professor, 歯学部, 助教授 (70266568)
INOUE Takashi Tokyo Dental College, Professor, 歯学部, 教授 (20125008)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | MTA / Calcified Tissue Formation / Rat Experimental Model / Rat Bone Marrow Stromal Cell / Immuno-Histlogical Staining / Bone Related Proteins / ラット属髄細胞 / 骨連タンパク / 硬組織形成能 / ラット頭蓋骨モデル / 病理組織学的検討 / 免疫組織化学的検討 |
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| Research Abstract |
Aim of these consecutive studies was to investigate biological effects of Mineral Trioxide Aggregate(MTA) to calcified tissue formation from in vivo and in vitro assessments.
Experiment 1 : Immuno-histological assessment of new calcified tissue formation promoted by MTA on Rat experimental model
First of all, the in vivo study with a rat experimental model has been performed in orda to evaluate effect of MTA for new bone formation. In this study, the ability of calcified tissue formation after the implantation of MTA in a rat parietal bone. IRM(Intermediate Restorative Material) was used as a control. MTA was encapsulated by the fibrous csonnective tissue in which PCNA and Cbfa-1 positive cells with some new calcified tissue formation was immuno-histologically observed. Otherwise, IRM was also encapsulated by the fibrous connective tissue, but any calcified tissue formation and Cbfa-1 positive cells were not founded. Those phenomena suggested that MTA might induce the difierentiation of
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undifferentiated pre-osteoblastic cells to osteogenic cells and on the other hand, IRM might be a low biocompatible material.
Experiment 2 : Behaviour of osteoblast-like cells on MTA associated with expression of type I collagen and bone related protein mRNAs
Secondary, the in vitro study was performed to investigate a behaviour of rat bone marrow cells(RBM), osteoblast-like cells, on MTA compared with IRM. RBM obtained from rat femur and cultured were used in this study. The cultured RBM were seeded on each material, and they were evaluated morphologically using scanning(SEM) and transmission(TEM) electron microscopies. Furthermore, the calcium released hydrorated material, the cell proliferation ratio and alkaline phosphatase(ALP) activity were analyzed, and the expression of type I collagen and bone related protein mRNAs were evaluated.
SEM showed that RBM attached to MTA and had a flattened appearance without nuclear protrusions and microspikes. TEM showed that the cells attached in the same manner as the control group, but gaps lager than 2 μm were frequently observed. The calcium released from hydrated MTA was about 130 ppm after 3days of immersion in saline. The ALP activity was similar to the control group. Cell proliferation and expression of type I collagen mRNA was significantry lower, while the expression of osteopontin mRNA was significantly higher than the control group at the 3^<rd> day of culture. In IRM groups, a few rounded cells were observed on the material but no living cells were seen. Less
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