| Project/Area Number |
15592059
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
NAGAMATSU Hiroshi Kyushu Dental College, Division of Comprehensive Dentistry, Assistant Researcher, 歯学部, 助手 (70275444)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOKAWA Ryuji Kyushu Dental College, Division of Oral Reconstruction and Rehabilitation, Professor, 歯学部, 教授 (60211546)
KOJO Tatsuro Kyushu Dental College, Division of Oral Reconstruction and Rehabilitation, Lecturer, 歯学部, 講師 (80153542)
FUKUIZUMI Takaki Kyushu Dental College, Division of Infection and Molecular Biology, Lecturer, 歯学部, 講師 (50275442)
INOUE Hiromasa Kyushu Dental College, Division of Infection and Molecular Biology, Assistant Professor, 歯学部, 助教授 (20137326)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | mucosal immunity / Candida albicans / palatine tonsil / immunization / 唾液 / IgA / カンジダ / 義歯性口内炎 / ワクチン |
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| Research Abstract |
To examine the possibility of a vaccine for candidiasis, we induced salivary antibodies to Candida albicans by weekly-applications of killed-C.albicans onto palatine tonsils of rabbits for six weeks. Significant levels of antibodies to C.albicans were seen in saliva and blood plasma by direct agglutination and ELISA. The saliva after immunization greatly inhibited C.albicans adherence to cloned human epithelial cells, while the saliva before immunization had no effect on the adherence. From the results of selective inhibition in ELISA reaction and agglutination by commercial antibodies to C.albicans, around 80% of the salivary antibodies were shown to react with one of the mannoprotein antigens, called factor 6 which has been shown to be involved of C.albicans adherence to epithelial cells. Therefore, tonsillar applications of C.albicans induce salivary antibodies that prevent C.albicans adherence to epithelial cells, and thus should prove useful in prevention of oral candidiasis.
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