| Project/Area Number |
15592065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
YOSHINARI Masao TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, ASSOCIATE PROFESSOR, 歯学部, 助教授 (10085839)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Takashi TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, PROFESSOR, 歯学部, 教授 (20125008)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Dental implant / Bisphosphonate / Bone regeneration / Hydroxyapatite / Drug delivery system / Sintering temperature / Local administration / Crystallinity |
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| Research Abstract |
Bisphosphonates (Bps) have been used as an effective drug for patients suffer from osteoporosis. The Bps chemically binds to the calcium phosphate in the bone and suppresses the activation of osteoclasts. In addition, Bps is known to inhibit bone resorption by directly facilitating osteoclast apoptosis.
It was revealed that the Bps chemically binds to the hydroxyapatite (HAp) by FT-IR analysis. The efficacy of hydroxyapatite (HAp) as a carrier was investigated in order to establish a method of local administration of bisphosphonates (Bps), which has currently been administered systemically. HAp granules 300-500 μm in size were prepared with different physicochemical features by altering the sintering temperature. To ascertain the physicochemical properties of the HAp granules, their crystallinity was assessed using X-ray diffraction, the surface morphology was examined under scanning electron microscopy, and the specific surface area and calcium dissolution were evaluated. Different Bps-HAp composites were subsequently prepared and the concentration of Bps released from these composites was measured. The influence of Bps-HAp composites on the rate of osteoclast survival was also evaluated. The results revealed that 1) HAp solubility depends on the sintering temperature ; 2) The concentration of released Bps could be controlled by regulating the sintering temperature of HAp as a carrier ; and 3) Bps released from Bps-HAp composites reduced the number of osteoclasts. These findings indicated that Bps-HAp composites could be locally administered as a drug delivery system to areas with bone resorption.
Furthermore, the molecular precursor solution was prepared by the addition of dibutylammonium metaphosphate salt into the ethanol solution of Ca-EDTA/amine complex to establish the titanium-HAp scaffold with DDS. Carbonate-containing hydroxyapatite was deposited onto titanium scaffold using molecular precursor method, which was novel technique for coating metal oxide film.
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