| Project/Area Number |
15592073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Nihon University |
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Principal Investigator |
HAYAKAWA Tohru Nihon University, School of Dentistry at Matsudo, Lecture(Full-Time), 松戸歯学部, 講師 (40172994)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Masafumi Nihon University, School of Dentistry at Matsudo, Professor, 松戸歯学部, 教授 (80210558)
NAGAI Megumi Nihon University, School of Dentistry at Matsudo, Lecture(Full-Time), 松戸歯学部, 講師 (30343578)
YOSHINARI Masao Tokyo Dental College, Associate Professor, 歯学部, 助教授 (10085839)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | dental implant / cell-adhesive protein / Titanium / Collagen / Fibronectin / Tresyl Chloride / Gingival attachment / immobilization / 細胞培養 / 骨 / 表面改質 |
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| Research Abstract |
We aimed to immobilize the cell-adhesive proteins onto titanium for improving the bone and tissue response. Tresyl chloride treatment for titanium surface was effective for binding the cell-adhesive proteins onto titanium. Tresyl chloride activated the basic OH of titanium surface. The difference of oxidation conditions of titanium surfaces did not provide any influences for the immobilization of cell-adhesive protein onto titanium. The activation of basic OH of titanium surface by tresyl chloride treatment and the immobilization of cell-adhesive proteins onto titanium were confirmed by the measurement of X-ray photoelectron spectroscopy or Fourier-transform infrared spectroscopy. The measurement of binding proteins onto titanium using quartz-crystal microbalance technique revealed that greater amount of proteins could be immobilized onto titanium surface using tresyl chloride-activation technique. Semi-empirical molecular orbital calculation suggested that the tresyl chloride activation influenced the energy levels of frontier molecular orbital. The initial cell attachment of human gingival flbroblast to titanium was enhanced by the immobilization of cell-adhesive proteins onto titanium using tresyl chloride-activation technique.
In conclusion, tresyl chloride-activation technique was very useful technique for the immobilization of cell-adhesive proteins onto titanium.
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