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Bone regenerative medicine by introducing ets family of gene

Research Project

Project/Area Number 15592092
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHokkaido University

Principal Investigator

HIGASHINO Fumihiro  Hokkaido Univ., Grad.School of Dent., Inst., 大学院・歯学研究科, 助手 (50301891)

Co-Investigator(Kenkyū-buntansha) SHINDOH Masanobu  Hokkaido Univ., Grad.School of Dent., Asso.Prof., 大学院・歯学研究科, 助教授 (20162802)
TOTSUKA Yasunori  Hokkaido Univ., Grad.School of Dent., Prof., 大学院・歯学研究科, 教授 (00109456)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsEts family / E1AF / osteonontin / bone production / rergulation of gene expression / CBFA1 / ルシフェラーゼアッセイ
Research Abstract

Ets oncogene family plays an important role for basic biological events such as embryogenesis, differentiation, cell growth and apoptosis. Previously, we found that one of the ets family, E1AF, activates the expression of matrixmetalloproteases (MMPs), which is related to bone production. The objective of this research is to examine whether ets genes induce the differentiation of osteoblast.
We produced an E1AF expression vector and examined the activation of osteopontin gene expression by luciferase assay. The expression was activated by E1AF in dose-dependent manner.
Since E1AF protein failed to be detected in the cells expressing E1AF stably, whereas the mRNA was expressed well, E1AF is regulated in post-transcriptional level. We found that E1AF is degraded by ubiquitin-proteasome pathway.
We examined the effect of adenovirus E1A, which inhibits bone production, on the activation of gene expression mediated by E1AF. The data showed that E1A inhibited the transcription from the gene targeted by E1AF.
These findings indicate that E1AF has some relation with bone production.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (6 results)

All 2005 2004 2003

All Journal Article (6 results)

  • [Journal Article] E1AF degradation by a ubiquitin-proteasome pathway.2005

    • Author(s)
      高橋暁子, 他
    • Journal Title

      Biochemical and Biophysical Research Communications 327

      Pages: 575-580

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] E1AF degradation by a ubiquitin-proteasome pathway.2005

    • Author(s)
      Takahashi A, Higashino F, Aoyagi M, Yoshida K, Itoh M, Kobayashi M, Totsuka Y, Kohgo T, Shindoh M.
    • Journal Title

      Biochem.Biophys.Res.Commun. 327

      Pages: 575-580

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] E1AF, an ets family transcription factor.2004

    • Author(s)
      進藤正信, 他
    • Journal Title

      Cancer Letter 216

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] E1AF, an ets family transcription factor.2004

    • Author(s)
      Shindoh, M, Higashino, F, Kohgo, T.
    • Journal Title

      Cancer Letter 216

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bisphenol A from dental polycarbonate crown upregulates the expression of hTERT.2004

    • Author(s)
      高橋暁子, 他
    • Journal Title

      Journal of Biomedical Materials 71B

      Pages: 214-221

    • Related Report
      2004 Annual Research Report
  • [Journal Article] EWS/ETS fusions activate telomerase in Ewing's tumors.2003

    • Author(s)
      高橋暁子, 他
    • Journal Title

      Cancer Research 63

      Pages: 8338-8344

    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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