| Project/Area Number |
15592105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nagoya University |
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Principal Investigator |
HIBI Hideharu Nagoya University, University Hospital, Associate Professor, 医学部附属病院, 助教授 (90345885)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Minoru Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (00151803)
MIZUNO Hirokazu Nagoya University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (70362241)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | distraction osteogenesis / distraction histogenesis / cultured osteoblasts-like cell / platelet-rich plasma / bone regeneration / mesenchymal stem cell / 骨形成 |
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| Research Abstract |
Distraction osteogenesis provides bone regeneration without grafting procedures but requires a long treatment time and the outcome depends on the condition of the periosteum. To promote bone formation and shorten the consolidation period, tissue-engineered osteogenic material was applied to distraction models of the canine and rabbit mandibles. The mandibles were cut and distracted over 10 mm long distance at rates of over 1 mm per day with latent periods of 0 or 1 day. The osteogenic material, which comprised autologous platelet-rich plasma and mesenchymal stem cells isolated from the iliac bone marrow aspirates, cultured, and induced to an osteogenic character, was infiltrated into the distracted tissue through a needle at the end of distraction. Series of radiograms showed that radiopacity in the distraction gap partly appeared at 1 week after distraction, wholly increased at 2 weeks and compared to that of the surrounding bone at 4 weeks. On histologic examination of tissue specimens in the distracted zone revealed mature lamellar bone with abundant osteocytes throughout. Distraction osteogenesis assisted with tissue-engineering was effective in promoting bone formation in the mandibles.
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