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THE EVALUATION OF OSTEOGEMC POTENTIAL OF CULTURED PERIOSTEUM-DERIVED CELLS

Research Project

Project/Area Number 15592109
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

UENO Takaaki  OKAYAMA UNIVEERSITY, GRADUATE SCHOOL OF MEDICINE AND DENITSTRY, ORAL AND MAXILLOFACIAL RECONSTRCUIIVE SURGERY, ASSISTANT PROFESSOR, 大学院・医歯学総合研究科, 助手 (60252996)

Co-Investigator(Kenkyū-buntansha) KAGAWA Toshimasa  OKAYAMAUNIVIRSITY MEDICALAND DENTAL HOSPITAL, ORAL AND MAXILLOFACIAL, RECONSTRCUTIVE SURGERY, ASSISTANT PROFESSOR, 医学部・歯学部附属病院, 助手 (80346444)
中村 浩彰  岡山大学, 大学院・医歯学総合研究科, 助教授 (50227930)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsPeriosteal graft / Cultured periosteum derived cell / Bone morphogenetic protein-4 / Bone repair / エレクトロポレーション / 遺伝子導入 / BMP-4遺伝子 / ラット骨膜細胞 / 培養細胞 / 細胞移植 / 骨形成能
Research Abstract

Periosteum has osteogenic potential and considerable attention has been focused on its use as a grafting material for the repair of bone and joint defects. Recently, the osteogenic potential of cultured periosteal cells has been reported. We present findings of bone formation induced from cultured periosteum-derived cells of rats tibiae. Periosteum was placed in culture medium with 10% Fetal Bovine Serum for 14 days. After reaching confluence, periosteal cells were resuspended with 025% trypsin/EDTA and then re-cultured three dimensionally on a collagen sponge for 7 days. Periosteal cell/collagen complex was grafted into rat submandibular muscle and A solution of plasmid DNA containing mouse BMP-4 (pMiw-BMP-4, kindly provided by Dr.Brigid I.M. Hogan and Dr.Kishimoto, Tohoku Univ.) was injected into grfated tissue and electric pulses were applied through paired-needle electrodes. As a control plasmid, Normal saline solution was injected. Immunosuppressant (FK506, 1.0 mg/kg/day) was administered intramuscularly. At 14, 21 and 35 days post grafting, grafted tissue was extirpated and compared histologically and radiographically with control groups. In the experimental group, periosteal cells had proliferated and differentiated into osteogenic cells by 14 days post grafting. Cell proliferation marker PCNA positive cells significantly increased compared with control groups. At 21 days post grafting, new bone formation was evident. At this stage, VEGF and BMP-4 positive cells significantly increased compared with control groups. By 35 days post grafting, bone growth was observed and new calcification was detected radiographically in the defect. New bone formation in experimental group developed and total calcified area was larger than control group. Cultured periosteum-derived cells treated with BMP-4 gene showed higher osteogenic potential in the early stage of periosteal cell graft model of rat.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (24 results)

All 2005 2004 2003 Other

All Journal Article (17 results) Book (1 results) Publications (6 results)

  • [Journal Article] Osteogenic Potential of Primed Periosteal Graft in the Rat Calvarial Model2005

    • Author(s)
      Kanou M, Ueno T, Kagawa T.et al.
    • Journal Title

      Annals Plastic Surgery 54

      Pages: 71-78

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic Potential of Primed Periosteal Graft in the Rat Calvarial Model2005

    • Author(s)
      Kanou M, Ueno T, Kagawa T. et al.
    • Journal Title

      Annals Plastic Surgery 54

      Pages: 71-78

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Regulation of bone metabolism in immunosuppresant FK506-treated rats.2004

    • Author(s)
      Kirino S, Fukunaga J, Ueno T et al.
    • Journal Title

      J Bone Mineral Metab 22

      Pages: 554-560

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Osteoblastoma of the mandible : Report of a Case with Immunohistochemical Analysis.2004

    • Author(s)
      Fujii T, Ueno T, Kagawa T et al.
    • Journal Title

      Asian Journal Oral Maxillofac Surg. 16

      Pages: 59-62

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Pathological change of articular cartilage in the mandibular head treated with immunosuppresant FK506.2004

    • Author(s)
      Ueno T, Kagawa T, Kanou M, et al.
    • Journal Title

      Histology and Histopathology 19

      Pages: 15-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Immunolocalization of VEGF During Heterotopic Bone Formation Induced From Grafted Periosteum.2004

    • Author(s)
      Ueno T, Kagawa T, Kanou M, et al.
    • Journal Title

      Annals Plastic Surgery. 53

      Pages: 150-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immunohistochemistry expression of Sox9 in the periosteum of the tibia and calvarial in after surgical release of the periosteum2004

    • Author(s)
      Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Takashi Toshio Sugahara
    • Journal Title

      Acta Histochemica (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteogenic Potential of Primed Periosteal Graft in the Rat Calvarial Model2004

    • Author(s)
      Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Toshio Sugahara
    • Journal Title

      Annals Plastic Surgery 46

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pathological change of articular cartilage in the mandibular head treated with immunosuppresant FK506.2004

    • Author(s)
      Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Takashi Toshio Sugahara
    • Journal Title

      Histology and Histopathology 19(1)

      Pages: 15-21

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immunolocalization of Vascular Endothelial Growth Factor During Heterotopic Bone Formation Induced From Grafted Periosteum.2004

    • Author(s)
      Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Takashi Toshio Sugahara
    • Journal Title

      Ann Plast Surg. 53(2)

      Pages: 150-154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immunolocalization of VEGF During Heterotopic Bone Formation Induced From Grafted Periosteum.2004

    • Author(s)
      Ueno T, Kagawa T, Kanou M, et al.
    • Journal Title

      Annals Plastic Surgery 53

      Pages: 150-154

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunohistochemical observation of cellular origin and proliferation in endochondral bone formation from rafted periosteum.2003

    • Author(s)
      Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Takashi Toshio Sugahara
    • Journal Title

      Journal of CranioMaxillofacial Surgery 31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Regeneartion of Mandibular Head from Grafted Periosteum.2003

    • Author(s)
      Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Takashi Toshio Sugahara
    • Journal Title

      Annals Plastic Surgery 51(1)

      Pages: 77-83

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pathology of the temporomandibular joint of patients with rheumatoid arthritis. -Case reports of secondary amyloidosis and macrophage populations.2003

    • Author(s)
      Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Takashi Toshio Sugahara
    • Journal Title

      Journal of CranioMaxillofacial Surgery 31(4)

      Pages: 252-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Vascular Endotherial Growth Factor (VEGF) and Bone Morphogenetic Protein-4 (BMP-4) in Endochondral Ossification from Grafted Periosteum.2003

    • Author(s)
      Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Takashi Toshio Sugahara
    • Journal Title

      Acta Histochem.Cytochem 36(1)

      Pages: 61-66

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immunohistochemical analysis of Sox9 in the periosteum of tibia nad calvaria after surgical release of perosteum.

    • Author(s)
      Fujii T, Ueno T, Kagawa T et al.
    • Journal Title

      Acta Histochemica (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immunohistochemical analysis of Sox9 in the periosteum of tibia nad calvaria after suraical release of perosteum.

    • Author(s)
      Fujii T, Ueno T, Kagawa T et al.
    • Journal Title

      Acta Histochemica (in press)

    • Related Report
      2004 Annual Research Report
  • [Book] 妊婦の歯科治療とカウンセリング妊婦歯科治療における留意点〜口腔外科の立場から〜2004

    • Author(s)
      分担執筆 植野高章
    • Total Pages
      400
    • Publisher
      東京臨床出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Publications] Ueno T, Kagawa T, Kanou M: "Pathological change of articular cartilage in the mandibular head treated with immunosuppresant FK506"Histology and Histopathology.. 19(1). 15-21 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueno T, Kagawa T, Kanou M: "Immunolocalization of Vascular Endotherial Growth Factor (VEGF) during Heterotopic Bone Formation Induced from Grafted Periosteum"Annals of Plastic Surgery. 50(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sawaki K., Yoshimoto T., Ueno T: "Mortality of human epidermal keratinocytes in co-culture with oral squamous cell carcinoma cells"Anticancer Research.. 23(1A). 79-84 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueno T, Kagawa T, Kanou M: "Immunohistochemical observation of cellular origin and proliferation in endochondral bone formation from rafted periosteum"Journal of CranioMaxillofacial Surgery. 31(6). 356-361 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueno T, Kagawa T, Kanou M: "Regeneartion of Mandibular Head from Grafted Periosteum"Annals of Plastic Surgery. 51(1). 77-83 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueno T, Kagawa T, Kanou M: "Pathology of the temporomandibular joint of patients with rheumatoid arthritis. -Case reports of secondary amyloidosis and macrophage populations"Journal of CranioMaxillofacial Surgery. 31(4). 252-256 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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