| Project/Area Number |
15592115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TOMIOKA Shigemasa The University of Tokushima, Institute of Health Bioscience, Department of Dental Anesthesiology, Associate professor, 大学院・ヘルスバイオサイエンス研究部, 助教授 (70188770)
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| Co-Investigator(Kenkyū-buntansha) |
SATOMURA Kazuhito The University of Tokushima, Institute of Health Bioscience, Department of Oral and Maxcillofacial Surgery, Associate professor, 大学院・ヘルスバイオサイエンス研究部, 助教授 (80243715)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | bone marrow stromal cell / differentiation / neuron / extracellular potential / NGF |
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| Research Abstract |
Bone marrow stromal cells (BMSCs) have recently been shown to have the capacity to differentiate into neurons. Nerve growth factor (NGF) which is one of neurotrophins has the effects to trigger long-term neuronal excitability. In the present study, to determine whether BMSCs-derived neurons acquire the electrical excitability, or whether an exposure to NGF induces or enlarges neuronal electrical excitability, we examine the effect of NGF for BMSCs-derived neurons using extracellular recording techniques. The neuron-like cells derived from murine BMSCs were confirmed to be stained with the specific neuronal marker Nestin, Neurofilament and Glial fibrillary acidic protein. Most BMSCs-derived neurons showed spontaneous discharges which amplitudes were up to 40 mV. When NGF at a concentration of 100 ng/ml was applied to BMSCs-derived neurons, the amplitudes of potentials were enlarged within 1 min and became greatest 3 min after NGF application. However, the enlargement of the amplitudes of potentials disappeared 5 min after NGF application. The similar effect by NGF was produced by the second application, but not by the third application. On the other hand, in the culture condition that little cell-cell contact were noted, enlargement of the amplitude of extracellular potentials was not observed even when NGF at a concentration of 100 ng/ml was applied a few times. These results indicate that NGF has the effect to induce transient but not sustained electrical excitability of neural cells derived from murine bone marrow stromal cells.
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