| Project/Area Number |
15592123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nara Medical University |
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Principal Investigator |
KAWAKAMI Tetsuji Nara Medical University, Oral and Maxillofacial Surgery, Assistant professor, 医学部, 講師 (60254512)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI Masayoshi Nara Medical University, Oral and Maxillofacial Surgery, Research Associate, 医学部, 助手 (20244717)
TANAKA Yasuharu Nara Medical University, Biochemistry, Associate Professor, 医学部, 助教授 (20124878)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Temporomandibular joint(TMJ) / Cyclooxygenase-2(COX-2) / Mechanical strech / Synovial fibroblasts / Nuclear factor-κB(NF-κB) / 顎関節症 / Cyclppxygenase-2(COX-2) / 滑膜細胞 / Nuclear factor-κB(NF-κB) / Cyclooxygenase-2(COX-2) |
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| Research Abstract |
It is proposed that synovial inflammation mediated by induced cyclooxygenase-2(COX-2) have important roles in the onset of temporomandibular joint(TMJ) pain. However, we have little knowledge about the pathophysiology of TMJ pain. In this study, the effect of uni-axial mechanical stretch on the expression of COX-2 was investigated in lapine synovial fibroblast cell line (HIG-82). Western blot analyses indicated that the level of the expression of COX-2 protein in HIG-82 increased in response to mechanical stretch (140%). We also observed nuclear translocation of p65 which is one of the nuclear factor-κB(NF-κB) family members in response to stretching. To investigate whether the induction of COX-2 was under NF-κB control was analyzed by luciferase reporter assay. Compared with the control, mechanical stretch treatment significantly increased the promoter activity of the NF-κB. These results showed that mechanical stress on synovial membrane induced COX-2 via activation of NF-κB. The present study suggested that synovial inflammation due to repetitive motion, such as bruxism might lead to TMJ pain.
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