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Relation of abnormal expression of cell cycle regulating factor and outcome of patients with COX-2 expression in the progression of oral carcinoma

Research Project

Project/Area Number 15592148
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHyogo College of Medicine

Principal Investigator

SAKURAI KAZUNARI  Hyogo College of Medicine, Medical Department, Assistant Professor, 医学部, 講師 (30129118)

Co-Investigator(Kenkyū-buntansha) URADE MASAHIRO  Hyogo College of Medicine, Medical Department, Professor, 医学部, 教授 (70104883)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsOral mucosa / Squamous cell carcinoma / Invasion and metastasis / COX-2 / Cell cycle regulating gene / Immunohistochemistry / In situ hybridization / Prognostic factor / 口腔 / 口腔粘膜 / 口腔癌(扁平上皮癌) / 湿潤・転移
Research Abstract

In the analysis of COX-2 expression with proliferaive activity in 200 oral squamous carcinoma (OSCC), COX-2 was guided on the occasion of metastasis early, and found that there were poor prognosis in patients in overexpression (74% of high-expression). In addition, COX-2 extremely high expressed in the top of an invasive foci in OSCC patients with poor outcome. Therefore, it was considered that the COX-2 related to tumor invasiveness, and was suggested that the COX-2 expression become a possible indicator in the outcome in patients with OSCC. We investigated p53-Rb pathway related to COX-2 expression and a trend of cell cycle regulator gene group to the subject next. Among of this analysis, HPV16 type was detected to 78%, and HPV18 type to 69% among COX-2 expression 45 examples again by p53 related to COX-2 expression and Brigati labelled in situ hybridization method for HPV16 type in 82 OSCC, COX-2 expression and 18 type about expected HPV-E6,E7 gene expression to each gene of Rb when … More we performed it. As a result of having examined the above generally, linked a high level of Topoll alpha LI in all both positive examples, overexpression of Rb protein, and p21/p53, cyclinB, D1,E overexpression were revealed, but the expression of p27 protein was not shown. As the result, cell cycle rapidly progress by failure of a regulator gene with COX-2 overexpression in a p53-Rb pathway through COX-2 pathway and raised an increase in OSCC than the above, but, in the example that HPV16/18 type was detected with COX-2 underexpression, possibility we restored p27 gene abnormality, and to evade was suggested by participation of an E6, E7 gene. Progress of OSCC accelerated, in COX-2 overexpression, it was considered that we participated in convalescence defectiveness by linking a significant expression lack of p27 protein. On the other hand, overexpression of HPV16/18 type and a p27 protein level were comparatively stable and, in COX-2 underexpression example, stopped an increase of cancer in a limited part, and possibility to keep convalescence comparatively well was suggested. From the above mentioned research results, COX-2 linked abnormality of a regulator gene in various cell cycles and was related to permeation of OSCC and metastasis and invasiveness, and it was thought that it could be it with a prognostic factor. Furthermore, it was recognized, and COX-2 received various oncogene abnormality, and mutual expression of COX-2 and Laminin-5 gamma 2 expression in 73% when we examined COX-2 and laminin-5 gamma 2, relevance of Integrin alpha expression with tumor invasiveness and metastasis, and possibility to acquisition of invasive activity and promotion of metastatic character was thought about. We want to go ahead through investigation about the role of invasiveness and metastasis of OSCC, identification of a COX-2 instruction factor, participation in COX-2 pathway in future. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (4 results)

All 2005 2004 2003 Other

All Journal Article (4 results)

  • [Journal Article] Promotion of cell differentiation, and suppression of cell growth and cyclooxy-genase-2 expression by differentiation inducing agent in human oral squamous carcinoma SCC25 cells.2005

    • Author(s)
      Kuroda J., Urade M., Kishimoto H., Noguchi K., Hashitani S., Sakurai K., Nishimura N., Hashimoto-Tamaoki T.
    • Journal Title

      International Journal of Oncology 26

      Pages: 361-367

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Increased expression of cyclooxygenase (COX) -2 in DMBA-induced hamster cheek pouch carcinogenesis and chemopreventive effect of a selective COX-2 inhibitor.2004

    • Author(s)
      Nishimura N., Urade M., Hashitani S., Noguchi K., Mannno Y., Takaoka K., Sakurai K.
    • Journal Title

      Journal of Oral Pathology and Medicine 66

      Pages: 234-243

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Apoptosis induction and enhancement of anticancer drugs by celecoxib, a selective cyclooxygenase-2 inhibitor, in human head and neck carcinoma cell lines.2003

    • Author(s)
      Hashitani S., Urade M., Nishimura N., Maeda T., Takaoka K., Noguchi K., Sakurai K.
    • Journal Title

      International Journal of Oncology 23

      Pages: 665-672

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prognostic significance of cyclooxygenase-2 and DNA topoisomerase II α expression in head and neck carcinoma.

    • Author(s)
      Sakurai K., Urade M., Noguchi K., Hashitani S., Takaoka K., Segawa E., Kishimoto H.
    • Journal Title

      Head and Neck (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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