| Project/Area Number |
15592159
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
OHSHIMA Kuniko Niigata University, Medical and Dental Hospital, lecturer, 医歯学総合病院, 講師 (80213693)
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| Co-Investigator(Kenkyū-buntansha) |
OHSHIMA Hayato Niigata University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (70251824)
HARADA Hidemitsu Osaka University, Graduate School of Dentistry, associate professor, 大学院・歯学研究科, 助教授 (70271210)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | adult stem cells / dental pulp / transplantation / replantation / regeneration / tooth development / BrdU / stress protein / Ki67 |
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| Research Abstract |
Rodent incisors are known to be continuously growing teeth that are maintained by both the cell-proliferation at the apical end and the attrition of the incisal edge. We propose a new concept that the eternal tooth bud producing various dental progeny is formed at the apical end of continuously growing teeth, and a new term "apical bud" for indicating this specialized epithelial structure. Furthermore, BrdU labeling analysis suggested that the guinea-pig molars, which were continuously growing teeth, also possessed plural specific proliferative regions and "apical bud" at the apical end.
The mechanism to determine the divergent pulpal healing process after tooth injury remains unclear. We investigated the healing process of dental pulp after tooth replantation by use of micro-computed tomography (μ-CT), immunocytochemistry for heat-shock protein (HSP)-25 and cathepsin K (CK), and histochemistry for both alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), In control teeth at postnatal 4 weeks, the periphery of coronal dental pulp showed intense ALP-and HSP-25-positive reactions, whereas no TRAP-and CK-positive cells occurred there. Tooth replantation weakened or ceased ALP-and HSP-25-positive reactions in the pulp tissue at the initial stages. Three to seven days after operation, ALP-positive region recovered from the root apex to the coronal pulp followed by HSP-25-positive reactions in the successful case leading to tertiary dentin formation. In another case, TRAP-and CK-positive cells appeared in the pulp tissue of replanted tooth at postoperative days 5-10,and remained to be associated with the bone tissue after 12-60 days. These data suggest that the appearance of TRAP-and CK-positive cells may be a trigger to induce bone tissue formation in the dental pulp.
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