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Study for the induction of the periodontal ligament cell apoptosis by mechanical stress

Research Project

Project/Area Number 15592163
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

TSUBOI Yoshiko  Okayama University, Dental Hospital, Research assistant, 医学部・歯学部附属病院, 助手 (50325122)

Co-Investigator(Kenkyū-buntansha) TAKANO Teruko (YAMAMOTO Teruko)  Okayama University Graduate School of medicine, dentistry and pharmaceutical science, Professor, 大学院・医歯学総合研究科, 教授 (00127250)
SUGAWARA Yasuyo  Okayama University Graduate School of medicine, dentistry and pharmaceutical science, Assistant Professor, 大学院・医歯学総合研究科, 助手 (70379775)
大矢 伸治  岡山大学, 医学部・歯学部附属病院, 医員
宮本 学  岡山大学, 大学院・医歯学総合研究科, 助手 (40252978)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsmechanical stress / periodontal ligament cells / apoptosis / macroarray / clone
Research Abstract

In the orthodontic treatment, the tooth moves in the alveolar bone by the mechanical stress induced by various devices. However, it still remains unclear that how the physical mechanical stress is replaced into the various biological events. To find a clue to clarify this mechanism, we tried to identify the genes expressed by mechanical stress in the human periodontal ligament(PDL) primary culture cells, using the cDNA array method.
The patient, who was diagnosed as tooth extraction from orthodontic reasons, was thoroughly informed about the objective of the experiments, and their consent was obtained. PDL tissue derived from a premolar of the patient was excised, and was placed onto flexible bottom plate. One month later, the heterogeneous cells reached confluence, and continuous mechanical stress was loaded onto the cells for 1, 6, 24 hours. Then, total RNA was extracted and purified. The total RNA was reverse transcribed into cDNA, and amplified by PCR method. Afterwards, it was labe … More led by ^<32>P and hybridized to the human array membrane. As a result, among 1176 genes studied, many genes were up-regulated or down-regulated within a wide range after 1 hour of stretching ; however, almost all of them returned to the control level by 6 or 24 h. The up-regulated or down-regulated genes at 1h stretching were related to cell cycle arrest and apoptosis, as well as cytokines, hormones, extracellular matrixes, adhesion moleclues, protein kinases, and others. Quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) for selected genes confirmed the results of the cDNA array analysis. We also demonstrated that the gene most markedly induced by continuous stretching and related to the cell cycle or apoptosis was in part activated via the p38 mitogen-activated protein kinase (MAPK) pathway, by Western blotting and the inhibitor experiment. This work has established an important baseline for clarifying the mechanisms whereby the PDL responds to mechanical stresses, using cDNA array analysis. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (8 results)

All 2005 2004 Other

All Journal Article (6 results) Publications (2 results)

  • [Journal Article] Three-dimensional reconstruction of chick calvaria osteocytes an their processes using confocal microscopy.2005

    • Author(s)
      Sugawara Y
    • Journal Title

      Bone (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Terminal differentiation of osteoblasts to osteocytes is accompanied by dramataic changes in the distribution of actin-binding proteins.2004

    • Author(s)
      Kamioka H
    • Journal Title

      J Bone Miner Res 19・3

      Pages: 471-478

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Terminal differentiation of osteoblasts to osteocytes is accompanied by dramataic changes in the distribution of actin-binding proteins.2004

    • Author(s)
      Kamioka H, Sugawara Y, Honjo T, Yamashiro T, Takano-Yamamoto T
    • Journal Title

      J Bone Miner Res 19(3)

      Pages: 471-478

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy.2004

    • Author(s)
      Sugawara Y, Kamioka H, Honjo T, Tezuka K, Takano-Yamamoto T
    • Journal Title

      Bone (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dennercation resulted in dento-alveolar ankylosis associated with decreased Malassez Epithelium.2004

    • Author(s)
      Fujiyama K
    • Journal Title

      J Dent Res 83・8

      Pages: 625-629

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Three-dimensional reconstruction of chick calvarial osteocytes and their processes using confocal microscopy.

    • Author(s)
      Sugawara Y
    • Journal Title

      Bone (In press)

    • Related Report
      2004 Annual Research Report
  • [Publications] T.Fukunaga et al.: "Connective tissue growth factor mRNA expression pattern in cartilages is associated with their type I collagen expression."Bone. 33. 911-918 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Kamioka et al.: "Terminal differentiation of osteoblasts to osteocytes is accompanied by dramatic changes in the distribution of actin-binding proteins."J Bone Miner Res. 19. 471-478 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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