| Project/Area Number |
15592171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Nara Medical University |
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Principal Investigator |
KAWAKAMI Masayoshi Nara Medical University, Department of Oral and Maxillofacial Surgery, Assistant professor, 医学部, 学内講師 (20244717)
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| Co-Investigator(Kenkyū-buntansha) |
WANAKA Akio Nara Medical University, Department of Anatomy, Professor, 医学部, 教授 (90210989)
RICHMAN Joy M. the University of British Columbia, CANADA, Department of Oral Health Sciences, Faulty of Dentistry, Associate professor
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | LIM homeobox / Lhx6 / L3 / Lhx8 / in situ hybridization / maxillary primordia / chick embryo / epithelial-mesenchymal interaction / cleft lip and palate / Lhx8 / 顔面突起 / 組織パターン / 上皮-間葉系相互作用 / 形態発生 / LIM homeobox遺伝子 |
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| Research Abstract |
LIM-homeobox containing (Lhx) genes constitute transcriptional regulators that play critical roles in the control of pattern formation and cell type specification. We have identified two genes of chicken Lhx genes, designated Lhx6 and L3/Lhx8, which were widely expressed in the craniofacial region. Whole-mount in situ hybridization showed Lhx6 and L3/Lhx8 were expressed from Stage 14 to 31 in restrict domains of the maxillary and mandibular processes but not in the frontonasal mass. Frozen sections revealed these signals in the mesenchyme underneath the epithelium. To determine whether the expression of Lhx6 and L3/Lhx8 in the maxilla and mandibular primordia requires signals from the overlying oral epithelium, transplant of Stage 25 chick embryo maxilla/mandibular process into the limb bud was performed in which the mesenchyme was grown in the presence or absence of oral epithelium. The grafts of maxilla and mandibular mesenchyme with the epithelium resulted in significant levels of Lhx6 and L3/Lhx8 expressions. No expression of Lhx6 and L3/Lhx8 was still shown in those grafts of epithelium-free mesenchyme. In order to further examine the distribution of signaling molecules from oral epithelium, soaked bead in Fgf-8 or Tgf-β3 was implanted into either maxillary mesenchymal graft from which the epithelium had been removed. Tgf-β3 beads as well as Fgf-8 induced expressions of L3/Lhx8 in the grafts. Overall our data suggested that expression of Lhx6 and L3/Lhx8 in the facial primordia required the epithelial-mesenchymal interactions. Fgf-8 and Tgf-β3 may be capable of regulating the expression in the maxillary processes.
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