| Project/Area Number |
15592185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NOGUCHI Kazuyuki Tokyo Medical and Dental University, Graduate School, Research associate, 大学院・医歯学総合研究科, 助手 (90218298)
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| Co-Investigator(Kenkyū-buntansha) |
UMEDA Makoto Tokyo Medical and Dental University, Graduate School, Research Associate, 大学院・医歯学総合研究科, 助手 (90193937)
NAGASAWA Toshiyuki Tokyo Medical and Dental University, Graduate School, Research Associate, 大学院・医歯学総合研究科, 助手 (90262203)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | mouse / cementoblast / periodontopathic bacteria / LPS / IL-6 / COX-2 / PGE2 / IL-1 / IL-1α |
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| Research Abstract |
In the present study, we investigated whether a mouse cementoblast cell line, OC-CM 30 cells, stimulated with interleukin(IL)-1α or lipopolysaccharides (LPS) from Prophyomonas(P.) gingivalis and Actinobacillus(A.) actinomycetemcomitans produced COX-2-dependent PGE_2 and furthermore whether the produced PGE_2 affected IL-1α-induced IL-6 production. IL-1α and LPS from P.gingivalis and A.actinomycetemcomitans time-dependently induced PGE_2 production. Indomethacin (a COX-1/-2 inhibitor) and NS-398 (a COX-2 inhibitor) completely inhibited IL-1 α-and LPS-induced PGE_2 production. 17-phenyl-ω-trinor PGE_2 (an EP_1 agonist) and ONO-AE1-329 (an EP_4 agonist) mimicked PGE_2 enhancement of IL-1α-and LPS from P.gingivalis and A.actinomycetemcomitans induced IL-6 production in OC-CM 30 cells. Furthermore, we investgated the effect of PGE_2 on RANKL and OPG expression. PGE_2 did not enhance RANKL and OPG prtotein expression. From these data, we suggest that in OC-CM 30 cells IL-1α and LPS from P.gingivalis and A.actinomycetemcomitans induced PG_2 production in a COX-2-dependent manner and that the COX-2-derived PGE_2 up-regulates IL-1α-and LPS from P.gingivalis and A.actinomycetemcomitans elicited IL-6 production via EP_1 and/or EP_4 receptors. PGE_2 and IL-6 produced by cementoblasts may be involved in the pathogenesis of periodontal disease.
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