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Effects of basic fibroblast growth factor on the remodeling of alveolar bone

Research Project

Project/Area Number 15592186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka University

Principal Investigator

SAHO Teruyuki  Osaka University, Dental Hospital, Instructor, 歯学部附属病院, 助手 (10263295)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Shinya  Osaka University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (70239490)
SHIMABUKURO Yoshio  Osaka University, Graduate School of Dentistry, Associate Professor, 大学院・歯学研究科, 助教授 (50231361)
KITAMURA Masahiro  Osaka University, Dental Hospital, Assistant Professor, 歯学部附属病院, 講師 (10243247)
池澤 一彦  大阪大学, 歯学部附属病院, 講師 (80294114)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsperiodontal tissue / alveolar bone / periodontal tissue regeneration / FGF-2 / osteoclast / RANKL / OPG / TRAP / RAW264
Research Abstract

In order to maintain the healthy periodontal tissue, adequate supportive alveolar bone volume is required. Continuous bone remodeling is regulated by orchestrated cellular functions between osteoblasts and osteoclasts in periodontal tissue. On the other hand, we have already demonstrated that topical application of fibroblast growth factor-2 (FGF-2) induced significant periodontal tissue regeneration.
In this study, we examined the effects of FGF-2 on osteoclastogenesis in periodontal tissues. Immunohistochemical analysis revealed that FGF-2 is distributed on the periodontal ligament cells, gingival fibroblasts and osteoclasts in the periodontal tissue of beagle dogs with naturally occurring periodontitis. In in vitro studies, we successfully induced multinucleated osteoclasts from murine myelomonocytic cells (RAW264) by stimulating the cells with soluble RANKL (receptor activator of NF-κB ligand) for 3 days. Furthermore, we demonstrated that FGF-2 had no direct effects on the osteoclast formation from RAW264 cells under the stimulation with soluble RANKL. Interestingly, however, the conditioned medium of FGF-2-stimulated murine clonal periodontal ligament cells (MPDL-22) and gingival fibroblasts (MG/B6) showed the inhibitory effects on the osteoclast formation, but did not influence the maturation of the osteoclasts. This inhibitory effect was restored by the pretreatment of conditioned medium with anti-OPG (osteoprotegerin) mAb. In addition, FGF-2 upregulated the OPG and RANKL mRNA expressions in MPDL-22 and MG/B6. These results suggest that FGF-2-induced OPG produced from periodontal tissue cells can inhibit osteoclast formation in the periodontal tissue and may in turn support the alveolar bone formation.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

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