| Project/Area Number |
15592188
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
YOSHINO Hiroshi Hiroshima University, Hospital, Assistant Prof., 病院, 講師 (50240338)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAGUCHI Hiroyuki Hiroshima University, Graduate School of Biomedical Science, Associate Prof., 大学院・医歯薬学総合研究科, 助教授 (10224750)
HINO Takamune Hiroshima University, Graduate School of Biomedical Science, Research Associate, 大学院・医歯薬学総合研究科, 助手 (20274102)
NAKAMURA Sigeo Hiroshima University, Graduate School of Biomedical Science, Research Associate, 大学院・医歯薬学総合研究科, 助手 (00325200)
HASEGAWA Naohiko Hiroshima University, Hospital, Research Associate, 病院・助手 (10346512)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | stress / HPA-axis / periodontal tissue / ストレス反応系 / HPA axis |
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| Research Abstract |
Hypothalamic-pituitary-adrenal-axis (HPA-axis) is the main response system for physiological stress. The last productive material of this system is cortisol. It has been reported that cortisol is in saliva and gingival crevicular fluid. And recently, it has been demonstrated that mRNA for HPA-axis components and protein in various peripheral tissues. In this study, we investigated whether there is HPA-axis like system in periodontal tissue or not.
(1)mRNA for Corticotropin releasing hormone (CRH), CRH receptor 1 (CRHR1), Proopiomelanocortin (POMC), Prohormone convertase-1 (PC-1), Adrenocorticotropic hormone receptor (ACTHR) and CYP11A1 were demonstrated in culture cells from human periodontal tissue.
(2)CRH and ACTH were produced by cultured human gingival fibroblasts and keratinocytes.
(3)CRH and ACTH were observed immunohistocemically in periodontal tissue. Positive reaction in tissue from periodontal patients were stronger than from healthy volunteers.
These results suggest that there is stress response system at the site of periodontal tissue and this system may work in the periodontitis locally
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