Development of endotoxin-detection reagents for periodontal treatments with human lactoferrin
| Project/Area Number |
15592198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
NAKASHIMA Keisuke Health Science University of Hokkaido, Periodontology and Endodontology, Associate Professor, 歯学部, 助教授 (80227785)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Satsuki Health Science University of Hokkaido, Periodontology and Endodontology, Lecturer, 歯学部, 講師 (50281283)
小鷲 悠典 北海道医療大学, 歯学部, 教授 (60014338)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Lactoferrin / Endotoxin |
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| Research Abstract |
1.Binding capability to LFS: Inhibitory effects of LPS-IBP binding were measured for lactoferrin-derived peptides on the basis of polymyxin B. Inhibitory effects of human lactofenin, (hLf), hLf33K and hLf33R were 0.2 nM, 0.08 nM and 0.05 nM, respectively. There were significant differences among the effects.
2.Effects on the inflammatory cytokine release from THP-1 cells : LPS from E cob was added to THP-1 cell culture with hLf, hIf33K or hLf33R, and TNF-a levels and LDH activity in culture supernatant were measured TN-(x levels and LDH activity were significantly decreased upon addition of hLf, hLf33K or hLf33R, however, there was no significant difference among the inhibitory effects. Cell growth of THP-1 measured by cellular intake of dyes was not affected with any reagents.
3.Effects on chemotaxis of THP-1 cells : THP-1 cells were stimulated with LPS from E cob and chemotaxis was determined by Boyden chamber method. Chemotaxis were significantly decreased upon addition of hLf, hLf33K or hLf33R, however, there was no significant difference among the inhibitory effects.
4.Selection of detection dye and binding of the dye to hLf We consulted Kiriya Chemical Ca about the binding method of the food dye to hLf. They told that approved food dyes once bound to hLf would be no longer allowed to use for food In addition, we consulted Sigma Genosys Co. about the possibility of binding the dye to hLf-derived peptides during synthesis It seemed to be difficult to synthesize the peptide with the dye. We would explore the dye that binds directly to LPS in future.
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Report
(3 results)
Research Products
(4 results)
