A basic studies to develop the immune therapy for periodontal diseases by employing glycolipids derived from periodontal bacteria as antigens.
| Project/Area Number |
15592199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Hyogo College of Medicine (2004)
Saitama Medical University (2003) |
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Principal Investigator |
OHYAMA Hideki Hyogo College of Medicine, Faculty of Medicine, Assistant Professor, 医学部, 講師 (90280685)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSHITA Sho Saitama Medical School, Medicine, Professor, 医学部, 教授 (50167649)
UEMURA Yasushi Saitama Medical School, Medicine, Research Associate, 医学部, 助手 (40364781)
NISHIMURA Fusanori Okayama University, Graduate School of Medicine and Dentistry, Associate Professor, 大学院・医歯学総合研究科, 助教授 (80208222)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | glycolipid antigens / T cell lines / dendritic cells / periodontal bacteria / immune therapy |
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| Research Abstract |
This study is the first trial to establish the immune therapy for periodontal diseases by using glycolipid antigens derived from periodontal bacteria. It has been reported that the existence of T cells recognize the glycolipid antigens in context of CD1 molecules expressed on dendritic cells(DCs). These phenomena led us to have a hypothesis that the immune responses against glycolipids from periodontal bacteria were involved in the host defense of periodontal patients. To clarify the possibility of this issue, we tried to identify the glycolipid antigens derived from periodontal bacteria, CD1 molecules and T cell populations. We succeeded in obtaining the glycolipids showing high polarity derived from sonic extracts of three bacteria, including A.actinomycetemcomitans, P.gingivalis and T denticola. These glycolipids were mixed and added in the culture of DCs to be presented efficiently by CD1 molecules expressed on DCs. We co-cultured T cells from healthy volunteers with these DCs derived from irrelevant donors for several weeks. As a result, we succeeded in establishing 16 T cell lines from 3 healthy donors, which showed specific response against glycolipid antigens derived from these bacteria. To identify the glycolipid antigens inducing T cell proliferative responses, we separated the extract materials into several fractions and evaluated T cell proliferative responses in the presence of each fraction as antigen. As a result, it was revealed that there were several fractions inducing T cell responses, and that most of T cells induced by these fractions were CD4 positive. These findings elucidated that several glycolipids derived from periodontal bacteria would be possible antigens to induce the proliferative responses of CD4 positive T cells.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Polymorphism of the 5' flanking region of the IL-12 receptor β2 gene partially determines the clinical types of leprosy through impaired transcriptional activity.2005
Author(s)
Ohyama, H., Ogata, K., Takeuchi, K., Namisato, M., Fukutomi, Y., Nishimura, F., Naruishi, H., Ohira, T., Hashimoto, K., Liu, T., Suzuki, M., Uemura, Y., Matsushita, S.
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Journal Title
Journal of Clinical Pathology (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Role of helper T cells in the humoral immune responses against 53-kDa outer membrane protein from Porphyromonas gingivalis.2005
Author(s)
Kato, N., Ohyama, H., Nishimura, F., Matsushita, S., Takashiba, S., Murayama, Y.
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Journal Title
Oral Microbiology and Immunology 20(2)
Pages: 112-117
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Positional effect of amino acid replacement on peptide antigens for the increased IFN-γ production from CD4 T cells.2005
Author(s)
Liu, T., Kohsaka, H., Suzuki, M., Takagi, R., Hashimoto, K., Uemura, Y., Ohyama, H., Matsushita, S.
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Journal Title
Allergology International 54(1)
Pages: 117-122
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Monocytes of distinct clinical types of leprosy are differentially activated by cross-linking class II HLA molecules to secrete IL-12.2004
Author(s)
Ohyama, H., Kato, N., Takeuchi, K., Soga, Y., Uemura, Y., Nishimura, F., Matsushita, S.
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Journal Title
APMIS 112(4-5)
Pages: 271-274
Description
「研究成果報告書概要(欧文)」より
Related Report
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