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Smoking effects on human PMN in periodontal tissue

Research Project

Project/Area Number 15592200
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionNippon Dental University

Principal Investigator

ITO Hiroshi  Nippon Dental University, School of Dentistry at Tokyo, Associate Professor, 歯学部, 助教授 (30184683)

Co-Investigator(Kenkyū-buntansha) NUMABE Yukihiro  Nippon Dental University, School of Dentistry at Tokyo, Professor, 歯学部, 教授 (90198557)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsperiodontitis / smoking / Human Polymorphonuclear Leucocytes / human PMN elastase / nicotine / cotinine / degranulation / Active Oxygen Production
Research Abstract

The relationship between periodontal degradation and smoking has been demonstrated by epidemiological evidence. However, the mechanism of this effect is unknown, and may be related to a host-response reaction. We investigated the effect of smoking on periodontal tissue. Peripheral blood neutrophils are a front line response in the body's defense mechanism, and we exposed these to one of the toxic substances contained in tobacco smoke, nicotine, cotinine (a metabolite of nicotine having a half-life of which ranges from 20 to 40 hours), PMA, and LPS. We then measured the degranulation, elastase activity, total elastase, elastase-alpha-1-antitrypsin complex, alpha-1-antitrypsin and lactoferrin, and the active oxygen production by using cytoenzymology, enzyme assay, and ELISA. The results suggest that nicotine made PMN to hyperactive, but cotinine hypofunctional. We speculate that cotinine was one of the factors in periodontal degradation, because cotinine's half-life ranges from 20 to 40 hours. A relationship between serum cotinine and periodontal degradation has recently been reported, and we are investigating whether there was a correlation between gingival neutrophil activity and saliva cotinine. Furthermore, there is a possibility that the measurement of saliva cotinine may be a convenient biochemical marker. We published the results in the Japanese journal of conservative dentistry, Vol.48, No.6, 891-901, 2005.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (7 results)

All 2005 2004 Other

All Journal Article (5 results) Publications (2 results)

  • [Journal Article] 喫煙が好中球の機能に及ぼす影響-脱顆粒と活性酸素産性能についての一考察-2005

    • Author(s)
      伊藤弘, 沼部幸博
    • Journal Title

      日本歯科保存学雑誌 第48巻・6号

      Pages: 891-901

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Smoking Effects on Degranulation and Active Oxygen Production in Human Peripheral Polymorphonuclear Leucocytes2005

    • Author(s)
      Hiroshi ITO, Yukihiro NUMABE
    • Journal Title

      The Japanese journal of conservative dentistry Vol.48, No.6

      Pages: 891-901

    • NAID

      10017182066

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 喫煙が好中球の機能に及ぼす影響-脱顆粒と活性酸素産性能についての一考察-2005

    • Author(s)
      伊藤 弘, 沼部幸博
    • Journal Title

      日本歯科保存学雑誌 第48巻・6号

      Pages: 891-901

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 喫煙が抹消血好中球の殺菌能に及ぼす影響2004

    • Author(s)
      笹本宝, 伊藤弘, 沼部幸博, 鴨井久一
    • Journal Title

      日本歯科保存学雑誌 第47巻・第6号

      Pages: 803-814

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 喫煙が抹消血好中球の機能に及ぼす影響-脱顆粒と活性酸素産生能についての一考察-

    • Author(s)
      伊藤弘
    • Journal Title

      日本歯科保存学雑誌 (発表予定)

    • Related Report
      2004 Annual Research Report
  • [Publications] 伊藤 弘: "喫煙が抹消血好中球活性酸素産性能に及ぼす影響"日本歯科保存学雑誌. (発表予定).

    • Related Report
      2003 Annual Research Report
  • [Publications] 伊藤 弘: "喫煙が抹消血好中球の機能に及ぼす影響-脱顆粒と活性酸素産生能についての一考察-"日本歯科保存学雑誌. (発表予定).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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