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Development of an ultrasonic device to detect early demineralized lesions and establishment of clinical criteria for these diagnoses

Research Project

Project/Area Number 15592212
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionTohoku University

Principal Investigator

KOSEKI Takeyoshi  Tohoku University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (80291128)

Co-Investigator(Kenkyū-buntansha) IWAKURA Masaki  Tohoku University, Graduate School of Dentistry, Associate Professor, 大学院・歯学研究科, 助教授 (90005067)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsDental cries / Ultrasonic / Nondestructive test / Diagnosis
Research Abstract

The onset of dental caries is characterized by white-spots or staining on tooth surfaces. However, estimation of caries activities of these lesions is difficult because we have no tool for recognition which lesion is demineralizing, arresting or re-mineralizing. For detection of the initial changes of the tooth surface affected by dental caries on the enamel and root, we developed a noninvasive, nondestructive dental explorer employing ultrasonic sensor technology. The ultrasonic sensor detection system consists of an oscillating piezoelectric element for sensing, a piezoelectric detective element to obtain frequency characteristics of the tooth surface, and a lightweight arm for positioning the sensor on the tooth surface. Extracted teeth were collected with informed consent and demineralized enamel specimens were prepared with lactate-CMC gel (6%, pH 4.5). The ultrasonic sensor was loaded on these tooth surfaces and scanned for drawing a map of the frequency characteristics. The surface characteristics were represented by the frequency shifts of oscillation in the resonance method. The detecting element of the developed ultrasonic sensor was sufficiently small in the clinic. By measurements using four sets of enamel specimens, early lesions of enamel caries were dearly represented by low frequency shifts of oscillation in a demineralized time-dependent manner. By scanning surfaces of ten extracted teeth, this detection system was able to distinguish sound or caries lesions on the enamel and roots, calculus depositions, staining, white spots and cracks on the enamel surface. However, additional information was need when exploring complex surfaces with overlapping lesions. Our new sensory explorer employing the ultrasonic resonance method was found to be a powerful tool for estimating initial change and degree of progression of dental caries.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (24 results)

All 2004 2003 Other

All Journal Article (22 results) Publications (2 results)

  • [Journal Article] Detection of subgingival calculus and dentine caries by laser fluorescence2004

    • Author(s)
      Kurihara E, et al.
    • Journal Title

      Journal of Periodontal Research 39

      Pages: 59-65

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of caspase activation through release of cytochrome c from mitochondria in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans2004

    • Author(s)
      Kasai H, et al.
    • Journal Title

      FEMS Microbiology Letters 233

      Pages: 29-35

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A new automatic device for measuring the spinnbarkeit of saliva : the Neva Meter2004

    • Author(s)
      Gohara K, et al.
    • Journal Title

      Journal of Dentistry 32

      Pages: 335-338

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microbial etiology of periodontitis.2004

    • Author(s)
      Nishihara T et al.
    • Journal Title

      Periodontology 2000 36

      Pages: 14-26

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of subgingival calculus and dentine caries by laser fluorescence.2004

    • Author(s)
      Kurihara E, Koseki T, Gohara K, Nishihara T, Ansai T, Takehara T.
    • Journal Title

      Journal of Periodontal Research. 39(1)

      Pages: 59-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of caspase activation through release of cytochrome c from mitochondria in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans.2004

    • Author(s)
      Kasai H, Yamamoto K, Koseki T, Yokota M, Nishihara T.
    • Journal Title

      FEMS Microbiology Letters. 233(1)

      Pages: 29-35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A new automatic device for measuring the spinnbarkeit of saliva : the Neva Meter.2004

    • Author(s)
      Gohara K, Ansai T, Koseki T, Ishikawa M, Kakinoki Y, Shibuya K, Nishihara T, Takehara T.
    • Journal Title

      Journal of Dentistry. 32(4)

      Pages: 335-338

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Gamma-interferon enhances expression of CD14/MyD88 and subsequent responsiveness to lipopolysaccharide from Actinobacillus actinomycetemcomitans in human gingival fibroblasts.2004

    • Author(s)
      Mochizuki S, Kobayashi M, Suzuki T, Oikawa A, Koseki T, Nishihara T, Hasegawa K.
    • Journal Title

      Journal of Periodontal Research. 39(5)

      Pages: 333-343

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microbial etiology of periodontitis.2004

    • Author(s)
      Nishihara T, Koseki T.
    • Journal Title

      Periodontology 2000. 36

      Pages: 14-26

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microbial etiology of periodontitis.2004

    • Author(s)
      Nishihara T, Koseki T.
    • Journal Title

      Periodontol 2000. 36

      Pages: 14-26

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A new automatic device for measuring the spinnbarkeit of saliva : the Neva Meter.2004

    • Author(s)
      Gohara K, Ansai T, Koseki T, Ishikawa M, Kakinoki Y, Shibuya K, Nishihara T, Takehara T.
    • Journal Title

      J Dent. 32(4)

      Pages: 335-338

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Involvement of caspase activation through release of cytochrome c from mitochondria in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans.2004

    • Author(s)
      Kasai H, Yanamoto K, Koseki T, Yokota M, Nishihara T.
    • Journal Title

      FEMS Microbiol Lett. 233(1)

      Pages: 29-35

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection of subgingival calculus and dentine caries by laser fluorescence.2004

    • Author(s)
      Kurihara E, Koseki T, Gohara K, Nishihara T, Ansai T, Takehara T.
    • Journal Title

      J Periodontal Res. 39(1)

      Pages: 59-65

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of endothelin-1 in gingival epithelial cells2003

    • Author(s)
      Yamamoto E, et al.
    • Journal Title

      Journal of Periodontal Research 38

      Pages: 417-421

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth/differentiation factor-5 induces growth arrest and apoptosis in mouse B lineage cells with modulation by Smad.2003

    • Author(s)
      Nakahara T, et al.
    • Journal Title

      Cellular Signaling 15

      Pages: 181-187

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning and sequencing a HemK-family gene in Porphyromonas gingivalis2003

    • Author(s)
      Kusaba A, et al.
    • Journal Title

      DVA Sequence 14

      Pages: 71-74

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by E6 siRNA.2003

    • Author(s)
      Yoshinouchi M, et al.
    • Journal Title

      Molecular Therapy 8

      Pages: 762-768

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of endotheliin-1 in gingival epithelial cells.2003

    • Author(s)
      Yamamoto E, Awano S, Koseki T, Ansai T, Takehara T.
    • Journal Title

      Journal of Periodontal Research. 38(4)

      Pages: 417-421

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth/differentiation factor-5 induces growth arrest and apoptosis in mouse B lineage cells with modulation by Smad.2003

    • Author(s)
      Nakahara T, Tominaga K, Koseki T, Yamamoto M, Yamato K, Fukuda J, Nishihara T.
    • Journal Title

      Cellular Signalling. 15(2)

      Pages: 181-187

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cloning and sequencing a HemK-family gene in Porphyromonas gingivalis.2003

    • Author(s)
      Kusaba A, Ansai T, Akifusa S, Nakahigashi K, Inokuchi H, Takehara T.
    • Journal Title

      DNA Sequence. 14(1)

      Pages: 71-74

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by E6 siRNA.2003

    • Author(s)
      Yoshinouchi M, Yamada T, Kizaki M, Fen J, Koseki T, Ikeda Y, Nishihara T, Yamato K.
    • Journal Title

      Molecular Therapy. 8(5)

      Pages: 762-768

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Gamma-interferon enhances expression of CD14/MyD88 and subsequent responsiveness to lipopolysaccharide from Actinobacillus actinomycetemcomitans in human gingival fibroblasts.

    • Author(s)
      Mochizuki S, et al.
    • Journal Title

      Journal of Periodontal Research 39

      Pages: 333-343

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kurihara, E., Koseki, T., Gohara, K., Nishihara, T., et al.: "Detection of subgingival calculus and dentine caries by laser fluorescence"J Periodontal Res. 39・1. 59-65 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto, E., Awano, S., Koseki, T., Ansai, T., Takehara, T: "Expression of endothelin-1 in gingival epithelial cells"J Periodontal Res. 38・4. 417-421 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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