• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

社会性昆虫のカースト多型機構に関する分子生態学的研究

Research Project

Project/Area Number 15687001
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Ecology/Environment
Research InstitutionHokkaido University (2004-2005)
The University of Tokyo (2003)

Principal Investigator

三浦 徹  北海道大学, 大学院・地球環境科学研究院, 助教授 (00332594)

Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2005: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2004: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2003: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords杜会性昆虫 / カースト分化 / 後胚発生 / ボデイプラン / 遺伝子発現 / 成虫原基 / フェロモン / 幼若ホルモン / 社会性昆 / ボディプラン / 社会性昆虫 / 表現型多型 / シロアリ / アリ / 翅形成 / 形態形成
Research Abstract

これまでは,オオシロアリHodotermopsis sjostedtiの兵隊分化過程において,それまで同定を行った特異的発現遺伝子の発現動態などに関して,リアルタイム定量PCR装置を用いた発現動態の解析により,形態形成に重要な働きをすると考えられるいくつかの遺伝子の兵隊分化における発現動態を解析してきた.今年度は新たに,シロアリ類におけるゲノムサイズの推定や兵隊分化に伴う行動の変化とそれを司る神経系の改変機構の解明にも着手した.その結果,多くのシロアリの系統でのゲノムサイズが明らかとなり,今後行われて行くであろうゲノムプロジェクトヘの基礎を築いた.また,兵隊分化にともない特異的に巨大化するニューロンも同定され今後の社会行動の分子基盤解明に向けての展望が開けた.さらに,RNA干渉法などを用いた遺伝子機能の解析の開発にも着手した.現在も進行中であるが,幼若ホルモンで分化誘導をかけ,その過程で発現する遺伝子の機能の推定に大きな手助けとなる.
また,アリの社会性に関しても,これまで得られた,トゲオオハリアリDiacamma sp.やツヤオオズアリPheidole megacephala,カドフシアリMyrmecina nipponicaなどのカースト分化に関する知見を最大限活用し,杜会性の維持機構に迫るべく研究を遂行した.具体的には,トゲオオハリアリのカースト分化決定に関する神経系の改変機構をDifferential Display法により分子レベルで同定した.さらに同定された遺伝子をreal-time定量PCRで発現動態を解析し,行動改変の分子機構に関する洞察が得られた.
このようにアリとシロアリでそれぞれ得られたカースト分化と杜会行動に関する分子生物学的・発生整理学的知見を総括し,社会性を維持するための必須の分子機構とその進化に関する考察を行う.
本年度は8月に日本進化学会(仙台),3月に日本生態学会(新潟)に参加して研究成果を報告するとともに,国際的な科学雑誌にいくつかの重要な論文を公表することができた.

Report

(3 results)
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (18 results)

All 2005 2004 Other

All Journal Article (12 results) Publications (6 results)

  • [Journal Article] Screening of genes expressed in developing mandibles during soldier differentiation in the termite Hodotermopsis sjostedti2005

    • Author(s)
      Koshikawa S, Cornette R, Hojo M, Maekawa K, Matsumoto T, Miura T
    • Journal Title

      FEBS Letters 579

      Pages: 1365-1370

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Comparative analysis of caste differentiation during embryogenesis of social aphids whose soldier castes evolved independently.2005

    • Author(s)
      Ijichi N, Shibao H, Miura T, Matsumoto T, Fukatsu T
    • Journal Title

      Insectes Sociaux 52

      Pages: 177-185

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Morphological differences among three female castes: worker queen and intermorphic queen in the ant Myrmecina nipponica (Formicidae: Myrmicinae).2005

    • Author(s)
      Miyazaki S, Murakami T, Azuma N, Higashi S, Miura T
    • Journal Title

      Sociobioloby 46

      Pages: 363-374

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identitication of soldier-specific genes in the nasute termite Nasutitermes takasagoensis (Isoptera: Termitidae).2005

    • Author(s)
      Hojo M, Koshikawa S, Cornette R, Matsumoto T, Miura T
    • Journal Title

      Entomological Science 8

      Pages: 379-387

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A caste-specific cytochrome P450 in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Termopsidae).2005

    • Author(s)
      Cornette R, Koshikawa S, Hojo M, Matsumoto T, Miura T
    • Journal Title

      Insect Molecular Biology 15(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] シロアリにおけるカースト分化機構とその進化.2005

    • Author(s)
      三浦徹
    • Journal Title

      しろあり 142

      Pages: 3-8

    • NAID

      40007008975

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Apoptotic wing degeneration and formation of an altruism-regulating glandular appendage (gemma) in the ponerine ant Diacamma sp. from Japan.2005

    • Author(s)
      Gotoh A, Sameshima S, Tsuji K, Matsumoto T, Miura T
    • Journal Title

      Development, Genes and Evolution 215

      Pages: 69-77

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Developmental regulation of caste-specific characters in social-insect polyphenism.2005

    • Author(s)
      Miura T
    • Journal Title

      Evolution & Developmen 7

      Pages: 122-129

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of soldier caste-specific protein in the frontal gland of nasute termite Nasutitermes takasagoensis (Isoptera : Termitidae).2005

    • Author(s)
      Hojo M, Morioka M, Matsumoto T, Miura T
    • Journal Title

      Insect Biochemistry and Molecular Biology 35

      Pages: 347-354

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Proximate mechanisms and evolution of caste polyphenism in social insects : from society to genes.2004

    • Author(s)
      Miura T
    • Journal Title

      Ecological Research 19

      Pages: 141-148

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Comparative studies on alate wing formation in two related species of rotten-wood termites : Hodotermopsis sjostedti and Zootermopsis nevadensis.2004

    • Author(s)
      Miura T, Koshikawa S, Machida M, Matsumoto T
    • Journal Title

      Insectes Sociaux 51

      Pages: 247-252

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Wing disc development during caste differentiation in the ant Pheidole megacephala (Hymenoptera : Formicidae).2004

    • Author(s)
      Sameshima S, Miura T, Matsumoto T
    • Journal Title

      Evolution & Development 6

      Pages: 336-341

    • Related Report
      2004 Annual Research Report
  • [Publications] Garcia, J, Maekawa K, Miura T, Matsumoto T: "Genetic distance between Nasutitermes nigriceps and Nasutitermes corniger in Guatemalan using AFLP markers"Sociobiology. 41. 663-672 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miura T, Kashikawa S, Matsumoto T: "Winged presoldiers induced by a juvenile hormone analog in Zootermopsis nevadensis : implications for plasticity and evolution of caste differentiation"Journal of Morphology. 258. 22-32 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miura T, Braendle C, Shingleton A, Sisk G, Kambhampati S, Stern DL: "A comparison of parthenogenetic and sexual embryogenesis of the pea aphid Acyrthosiphon pisum (Hemiptera : Aphidoidea)"Journal of Experimental Zoology(Molecular Developmental Evolution). 295B. 59-81 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Koshikawa S, Matsumoto T, Miura T: "Mandibular morphogenesis during soldier differentiation in the damp-wood termite Hodotermopsis sjoestedti (Isoptera : Termopsidae)"Naturwissenschaften. 90. 180-184 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 三浦 徹: "シロアリの社会制御とカースト文化"遺伝別刷. 16. 43-50 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 三浦 徹: "環境にあわせた体のつくりかえ-表現型多型の仕組みと進化"遺伝. 57. 45-53 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi