ナノ制御されたミクロ表面構造インプラント

• Project/Area Number: 15791139
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 補綴理工系歯学
• Research Institution: Tokyo Dental College
• Principal Investigator: 松坂 賢一 東京歯科大学, 助教授 (70266568)
• Project Period (FY): 2003 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥2,500,000 (Direct Cost: ¥2,500,000); Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: microgroove / multi-groove / nano technology / osteocalcin / BMP / fbronectin / plasma spray / tissue engineering / mutli-groove / in vitro / fibronectin / plasma / ALP

Research Abstract

ミクロ制御として、表面構造が1〜10μmの幅と0.5〜1.5μmの深さを持つmicrogrooveを付与した培養基質上で骨性細胞を培養し、電子顕微鏡、共焦点レーザー顕微鏡による形態学的観察を行った結果、grooveに沿って細胞を伸展させ、さらには細胞が産生した石灰化基質の沈着もgrooveに沿って沈着させられ、幅が1、2μm上での細胞はridge上に強く接着していることが証明された。in vitroでの三次元的な骨組織構築を目的として、この細胞動態を利用して二重構造(台形groove:幅100μm+v字形microgroove:幅2μm)を有する基質(multi-groove)を作成し、in vitroにおける骨様組織の三次元的形態付与を実現させた。ナノ制御として、培養基質にプラズマ表面改質装置を用いた後にBMPあるいはfibronectin含有PBSを2時間留置した。その結果、蛍光抗体法を用いた方法によりBMPあるいはfibronectinが基質に固定されていることが確認され、Q-senseを用いた物理学的な方法により44.2ng/cm^2量のBMP、260.0ng/cm^2量が固定されていることが証明された。BMP固定培養基質上で、骨性細胞の培養実験を行い、有意に骨芽細胞の機能発現の指標となるALPの活性が上昇し、骨関連タンパクであるオステオカルシンのmRNAの発現も増加した。Fibronectin固定培養基質上での細胞接着に関しては、初期における接着細胞数が有意に多い結果となった。

Research Products

• [Journal Article] Bone formation in titanium porous scaffold with immobilization of BMP-2 (2005)
  • Author(s): Matsuzaka K, Yoshinari M, Kokubu E, Shimono M, Yamada Y, Mabuchi M, Inoue T
  • Journal Title: Journal of Oral Tissue Engineering 2・2 Pages: 60-65
  • NAID: 110006164161
• [Journal Article] Oseoblastic resorption of calcium phosphate coatings applied with electrostatic spray deposition (ESD), in vitro (2005)
  • Author(s): Sieber MC, Matsuzaka K, Walboomers XF, Leeuwenburgh SCG, Wolke JGC, Jansen JA
  • Journal Title: Journal of Biomedical Materials Research 74A Pages: 570-580
• [Journal Article] Behaviour of bone marrow osteoblast-like cells on mineral trioxide aggregate : morphology and expression of type I collagen and bone related protein mRNAs (2005)
  • Author(s): Nakayama A, Ogiso B, Tanabe N, Takeichi O, Matsuzaka K, Inoue T
  • Journal Title: International Endodontic Journal 38・4 Pages: 203-210
• [Journal Article] Bone formation in beta-Tricalcium phosphate-filled bone defects of the rat femur : morphometric analysis and expression of bone related protein mRNA (2005)
  • Author(s): Shiratori K, Matsuzaka K, Koike Y, Murakami S, Shimono M, Inoue T
  • Journal Title: Biomedical Research 26・2 Pages: 51-59
  • NAID: 130004470632
• [Journal Article] The effect of Emdogain on ectopic bone formation in tubes of rat demineralized dentin matrix (2005)
  • Author(s): Koike Y, Murakami S, Matsuzaka K, Inoue T
  • Journal Title: J Periodontal Res 40・5 Pages: 385-394
• [Journal Article] Characterization of the peri-implant epithelium in hamster palatine mucosa : Behavior of Merkel cells and nerve endings (2005)
  • Author(s): Suzuki Y, Matsuzaka K, Ishizaki K, Tazaki M, Sato T, Inoue T
  • Journal Title: Biomedical Research 26・6 Pages: 257-269
• [Journal Article] Periodontal ligament and gingival fibroblast adhesion to dentin-like textured surfaces. (2005)
  • Author(s): C.Bruckmann et al.
  • Journal Title: Biomaterials 26・4 Pages: 339-346
• [Journal Article] Behavior of osteoblast-like cells on fibronectin or BMP-2 immobilized surfaces. (2004)
  • Author(s): K.Matsuzak et al.
  • Journal Title: Biomedical Research 25 Pages: 263-268
• [Journal Article] Effects of multi-grooved surfaces on osteoblast-like cells in vitro: scanning electroninc microscopic observation and mRNA expression of osteopontin and osteocalcin (2004)
  • Author(s): K.Matsuzaka et al.
  • Journal Title: J.Biomedical Materials Res. 68 Pages: 227-234
• [Journal Article] The attachment and growth behavior of osteoblast-like cells on microtextured srufaces (2003)
  • Author(s): K.Matsuzaka et al.
  • Journal Title: Biomaterials 24 Pages: 2711-2719
• [Publications] K.Matsuzaka, et al.: "The effect of poly-l-lactic acid with parallel surface micro groove on osteoblast-like cells in vitro"Biomaterials. 20. 1293-1301 (1999)
• [Publications] K.Matsuzaka, et al.: "Effect of microgrooved poly-l-lactic (PLA) surfaces on proliferation, cytoskeletal organization, and mineralized matrix formation of rat bone marrow cells"Clin.Oral Implant Res.. 11. 325-333 (2000)
• [Publications] R.Mabuchi, et al.: "Cell proliferation and cell death in periodontal ligaments during orthodontic tooth movement"J.Periodontl.Res.. 37. 118-124 (2002)
• [Publications] M.Yoshinari, et al.: "Bone response to calcium phosphate-coated and bisphosphonate-immobilized titanium implant"Biomaterials. 23. 2879-2885 (2002)
• [Publications] K.Matsuzaka, et al.: "The attachment and growth behavior of osteoblast-like cells on microtextured surfaces"Biomaterials. 24. 2711-2719 (2003)
• [Publications] K.Matsuzaka, et al.: "Effects of multi-grooved surfaces on osteoblast-like cells in vitro : scanning electron microscopic observation and mRNA expression of osteopontin and osteocalcin"J.Biomedical Materials Res.. 68. 227-234 (2004)