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日本産河川昆虫カワゲラの種多様性の系統発生学的再評価:DNAと形態に基づく種分類

Research Project

Project/Area Number 15F15065
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Civil and environmental engineering
Research InstitutionEhime University

Principal Investigator

渡辺 幸三  愛媛大学, 理工学研究科(工学系), 准教授 (80634435)

Co-Investigator(Kenkyū-buntansha) MURANYI DAVID  愛媛大学, 理工学研究科(工学系), 外国人特別研究員
Project Period (FY) 2015-04-24 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2016: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2015: ¥800,000 (Direct Cost: ¥800,000)
Keywordsカワゲラ / 系統発生 / 分類学 / 河川生態系 / 系統地理 / 水生昆虫 / 進化 / 生物多様性 / 種分類 / 底生動物
Outline of Annual Research Achievements

本研究は、ヨーロッパ,北アメリカ,日本広域を網羅する東アジアから採取した成虫標本を用いて、カワゲラ目クロカワゲラ科の属ならびに種の定義を見直した。形態学的分析とDNA塩基配列に基づいて、(1)クロカワゲラ類の時間的,地理的な進化過程を明らかにすること,(2) クロカワゲラ類の属間の形態形質の進化パターンを明らかにすること, (3) DNAデータベースの充実化に向けクロカワゲラ類のCOI塩基配列とHistone H3塩基配列を解読し決定することを目的とした解析を行った.主な結論を以下にまとめる.
(1) クロカワゲラ科224サンプルから形態同定により未記載属7属,未記載種15種が確認された.形態だけでなく分子系統樹においてもこれらの属の単系統が示されたことにより,形態同定の正確性が裏付けられた.なお,未記載属Aのアメリカ産と日本産は系統の異なる別属であることが示唆された.日本産クロカワゲラ科のいずれの種も,現在のCapnia属の狭義の形態学的定義には当てはまらないことが明らかになった。これらは我々が定義しようとしている新たな属に分類されるものと考えられる。
(2) ベイズ推定法による分子系統樹からクロカワゲラ類の時間的,地理的な進化過程が推定された.日本産の属の多くが単系統群に当てはまり,日本産クロカワゲラは大きく分けて3つの系統から成ることが示された..
(3) 遺伝距離と形態形質の距離の間に正の相関があることが示された.クロカワゲラ科は遺伝的な差が大きくなるほど形態も異なっている.
(4) クロカワゲラ科19属62種のCOI配列を220配列,Apteroperla(ハダカカワゲラ)属6種のHistone H3配列を28配列解読した.これらの配列データをDNAデータベースに登録し,DNAバーコーディングの精度向上に役立てられるものと考えられる.

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (29 results)

All 2017 2016 2015 Other

All Int'l Joint Research (4 results) Journal Article (17 results) (of which Int'l Joint Research: 14 results,  Peer Reviewed: 17 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) (of which Int'l Joint Research: 6 results)

  • [Int'l Joint Research] ハンガリー自然史博物館/ハンガリー科学アカデミー植物保護研究所(ハンガリー)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Korea University(韓国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Hungarian Natural History Museum(ハンガリー)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Henan Institute of Science & Technology(中国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] New species and records of Leuctridae (Plecoptera) from Guangxi, China, on the basis of morphological and molecular data, with emphasis on Rhopalopsole2017

    • Author(s)
      Weihai Li, David Muranyi, Maribet Gamboa, Ding Yang, and Kozo Watanabe
    • Journal Title

      Zootaxa

      Volume: 4243(1) Issue: 1 Pages: 165-176

    • DOI

      10.11646/zootaxa.4243.1.8

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Two new species of Protonemura (Plecoptera: Nemouridae) from China, with biogeographical notes on the genus2017

    • Author(s)
      Li, W.H., Muranyi, D. & Yang, D.
    • Journal Title

      Zootaxa

      Volume: 4243(1) Issue: 1 Pages: 165-176

    • DOI

      10.11646/zootaxa.4258.1.4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A new species of Leuctra from Turkey, and notes on Anatolian Rhabdiopteryx (Plecoptera: Leuctridae & Taeniopterygidae)2017

    • Author(s)
      Muanyi, D. & Vincon, G.
    • Journal Title

      Zootaxa

      Volume: 4243(2) Issue: 2 Pages: 383-388

    • DOI

      10.11646/zootaxa.4243.2.9

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lost and found: the Plecoptera types of Blanchard and Mabille, with further contributions to the stoneflies of Chile2016

    • Author(s)
      Muranyi, D., Gamboa, M. & Vera, A.
    • Journal Title

      Zootaxa

      Volume: 4200(4) Issue: 4 Pages: 544-560

    • DOI

      10.11646/zootaxa.4200.4.6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] On the identity of some Oriental Acroneuriinae taxa (Plecoptera: Perlidae), with an annotated checklist of the subfamily in the realm2016

    • Author(s)
      Muranyi, D. & Li, W.H.
    • Journal Title

      Opuscula Zoologica Budapest

      Volume: 47(2) Issue: 2 Pages: 173-196

    • DOI

      10.18348/opzool.2016.2.173

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] New species records of systellognathan stoneflies (Plecoptera: Systellognatha) for the fauna of the Korean Peninsula2016

    • Author(s)
      Muranyi, D., Hwang, J.M., Jeon, M.J. & Seo, H.Y.
    • Journal Title

      Opuscula Zoologica Budapest

      Volume: 47(2) Issue: 1 Pages: 111-121

    • DOI

      10.18348/opzool.2016.2.111

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Contribution to the knowledge of Turkish stoneflies with annotated catalogue (Insecta: Plecoptera)2016

    • Author(s)
      DARILMAZ, M.C., SALUR, A., MURANYI, D. & VINCON, G.
    • Journal Title

      Zootaxa

      Volume: 4074(1) Issue: 1 Pages: 1-74

    • DOI

      10.11646/zootaxa.4074.1.1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Checklist of the Korean Plecoptera2015

    • Author(s)
      HWANG, J.M. & MURANYI, D.
    • Journal Title

      Entomological Research Bulletin

      Volume: 31(2) Pages: 120-125

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Review and contribution to the Plecoptera fauna of Sălaj county, Romania2015

    • Author(s)
      MURANYI, D. & KOVACS, T.
    • Journal Title

      Studia Universitatis Vasile Goldis Arad

      Volume: 25(4) Pages: 255-258

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Review and contribution to the Odonata fauna of Salaj county, Romania2015

    • Author(s)
      MURANYI, D., KATONA, G., FEKETE, J. & KOVACS, T.
    • Journal Title

      Studia Universitatis Vasile Goldis Arad

      Volume: 25(4) Pages: 250-253

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Contribution to the Opiliones fauna of Sălaj county, Romania2015

    • Author(s)
      MURANYI, D.
    • Journal Title

      Studia Universitatis Vasile Goldis Arad

      Volume: 25(4) Pages: 227-229

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A remarkable new genus of Perlodinae (Plecoptera: Perlodidae) from China, with remarks on the Asian distribution of Perlodinae and questions about its tribal concept2015

    • Author(s)
      LI, W.H. & MURANYI, D.
    • Journal Title

      Zoologischer Anzeiger

      Volume: 259 Pages: 41-53

    • DOI

      10.1016/j.jcz.2015.10.003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] First record of the genus Graecophalangium Roewer, 1923 (Opiliones: Phalangiidae) from Albania, with redescription of G. militare (C.L. Koch, 1839)2015

    • Author(s)
      MURANYI, D.
    • Journal Title

      Ecologica Montenegrina

      Volume: 4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Just like birdwatching: the first record of Megaperlodes tiunovi Teslenko, 2015 (Plecoptera: Perlodidae) from Korea2015

    • Author(s)
      MURANYI, D. & HWANG, J.M.
    • Journal Title

      Illiesia

      Volume: 11(10) Pages: 126-129

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New species records of Suwallia Ricker 1943 (Plecoptera: Chloroperlidae) from China, with description of the nymph of S. decolorata Zhiltzova & Levanidova, 19782015

    • Author(s)
      LI, W.H., MURANYI, D. & LI, S.
    • Journal Title

      Zootaxa

      Volume: 3994(4) Issue: 4 Pages: 556-564

    • DOI

      10.11646/zootaxa.3994.4.4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Klapalek’s Kamimuria (Plecoptera: Perlidae) types in the National Museum Prague2015

    • Author(s)
      MURANYI, D. & LI, W.H.
    • Journal Title

      Opuscula Zoologica Budapest

      Volume: 46(2) Issue: 2 Pages: 159-175

    • DOI

      10.18348/opzool.2015.2.159

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new genus and species of winter stoneflies (Plecoptera: Capniidae) from Southwest China, with a commented checklist of the family in the Oriental Realm2015

    • Author(s)
      MURANYI, D., LI, W.H. & YANG, D.
    • Journal Title

      Zootaxa

      Volume: 4059(2) Issue: 2 Pages: 371-382

    • DOI

      10.11646/zootaxa.4059.2.8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 日本産クロカワゲラ科の分類学的改訂 (Revision of the Capniidae (Plecoptera)2016

    • Author(s)
      Muranyi, D., Gamboa, M., Gotoh, J. & Watanabe, K
    • Organizer
      第40回水生昆虫研究会
    • Place of Presentation
      ASAHI LODGE(兵庫県・養父市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Different ways of sperm transfer among the Capniidae (plecoptera), and its importance in the generic system of the family2016

    • Author(s)
      David Muranyi, W.H. Li, M. Gamboa, K. Watanabe
    • Organizer
      The 3rd Symposium of Benthological Society of Asia
    • Place of Presentation
      Vladivostok, Rossia
    • Year and Date
      2016-08-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Systematic revision of the Asian Capniidae (Plecoptera)2016

    • Author(s)
      David Muranyi, Maribet Gamboa, Jeong Mi Hwang, Yeon Jae Bae, Kozo Watanabe
    • Organizer
      Annual Meeting of the Entomological Society of Korea
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2016-05-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Different ways of sperm transfer among the Capniidae (plecoptera), and its importance in the generic system of the family2016

    • Author(s)
      D. Muranyi, W.H. Li, M. Gamboa, K. Watanabe
    • Organizer
      The 3rd Symposium of Benthological Society of Asia
    • Place of Presentation
      Vladivostok, Rusia
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Systematic revision of the Asian Capniidae (Plecoptera)2016

    • Author(s)
      David Muranyi, Maribet Gamboa, Jeong Mi Hwang, Yeon Jae Bae, Kozo Watanabe
    • Organizer
      Annual Meeting of the Entomological Society of Korea
    • Place of Presentation
      Daegu, South Korea
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 日本の気候勾配に沿ったオナシカワゲラ(Nemoura sp.)個体群の比較プロテオーム解析2016

    • Author(s)
      山野俊介,Maribet Gamboa,Maria Claret Lauan,岩田久人,渡辺幸三
    • Organizer
      応用生態工学会第20回大会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Towards the generic revision of Capniidae (Plecoptera): synopsys of the East Palaearctic genera2015

    • Author(s)
      MURANYI, D., GAMBOA, M., LI, W.H. & WATANABE, K.
    • Organizer
      Meeting of the XIV International Conference on Ephemeroptera and XVIII International Symposia of Plecoptera
    • Place of Presentation
      Aberdeen, UK
    • Year and Date
      2015-05-31
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contribution to the taxonomy and biology of two Balkan endemic Isoperla Banks, 1906 (Plecoptera: Perlodidae) species2015

    • Author(s)
      MURANYI, D., KOVACS, T. & ORCI, K.M.
    • Organizer
      Meeting of the XIV International Conference on Ephemeroptera and XVIII International Symposia of Plecoptera
    • Place of Presentation
      Aberdeen, UK
    • Year and Date
      2015-05-31
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-11-26   Modified: 2024-03-26  

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