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Production of medium chain fatty acids (MCFA) using food waste

Research Project

Project/Area Number 15F15352
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Environmental engineering and reduction of environmental burden
Research InstitutionMuroran Institute of Technology

Principal Investigator

チャン ヨンチョル  室蘭工業大学, 工学部, 教授 (30422025)

Co-Investigator(Kenkyū-buntansha) MOTAKATLA VENKATESWER REDD  室蘭工業大学, 工学(系)研究科(研究院), 外国人特別研究員
MOTAKATLA VENKATESWER REDDY  室蘭工業大学, 工学研究科, 外国人特別研究員
Project Period (FY) 2015-10-09 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2017: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2016: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2015: ¥600,000 (Direct Cost: ¥600,000)
KeywordsMCFA / 中鎖脂肪酸 / 単鎖脂肪酸 / 嫌気微生物 / 鎖伸長 / C. kluyveri / 廃水 / 生ごみ
Outline of Annual Research Achievements

チーズ製造企業工房において生産過程ででるチーズホエーの有効活用は長年の課題である。そのため、チーズホエーを再利用し高付加価値化することができれば廃液処理コストの削減だけではなく、大手企業との価額競争力を高めることができる。このような中で我々は、13種類の嫌気微生物を集積し、中鎖脂肪酸(カプリル酸)の大量生産を試みた。しかし、生産量の向上はなかなか難しいことが分かった。一方、合成または実質廃棄物を用いた中鎖脂肪酸(MCFA)の製造については多く報告が存在するが、MCFA生成細菌を得るための集積手順について記載された報告はほとんどなかった。 したがって、本実験の目的は、嫌気性発酵によって合成される有効なMCFAを産生する潜在的な細菌叢を効果的に集積する方法の研究提案であった。 酢酸は嫌気性消化の主な中間生成物であるため、我々はフィジビリティスタディに用いる合成廃棄物の基質として酢酸を使用した。その結果、短鎖脂肪酸および中鎖脂肪酸を産生することができる細菌混合培養物が集積された。 集積された混合菌を用いて、酢酸およびエタノールから短鎖脂肪酸およびMCFAを製造した。また、 基質、電子供与体およびメタン阻害がMCFA生成に及ぼす影響について検討した。 その結果、様々な培養条件の中で、メタン生産阻害の条件で、酪酸塩、カプロン酸塩、およびカプリル酸塩の生産濃度が高くなることが分かった。一方、 CO2およびH2は、バイオガス組成成分の中で最も高い割合を示した。 DGGE分析によると、混合培養液中にはクロストリジウム、ルーメンバクテリウム、放線菌群に関連する細菌が優勢であったことが分かった。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (28 results)

All 2017 2016 2015 Other

All Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 6 results,  Open Access: 1 results) Presentation (15 results) (of which Int'l Joint Research: 12 results,  Invited: 1 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Medium-Chain Fatty Acids (MCFA) Production Through Anaerobic Fermentation Using Clostridium kluyveri: Effect of Ethanol and Acetate2017

    • Author(s)
      Reddy M. Venkateswar、Mohan S. Venkata、Chang Young-Cheol
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 185(3) Issue: 3 Pages: 1-12

    • DOI

      10.1007/s12010-017-2674-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Polyhydroxyalkanoates (PHA) production from synthetic waste using Pseudomonas pseudoflava: PHA synthase enzyme activity analysis from P. pseudoflava and P. palleronii2017

    • Author(s)
      Venkateswar Reddy M, Mawatari Y, Onodera R, Nakamura Y, Yajima Y, Chang YC
    • Journal Title

      Bioresource Technology

      Volume: 234 Pages: 99-105

    • DOI

      10.1016/j.biortech.2017.03.008

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Production of poly-3-hydroxybutyrate (P3HB) and poly-3-(hydroxybutyrate-co-hydroxyvalerate) P(3HB-co-3HV) from synthetic wastewater using Hydrogenophaga palleronii2016

    • Author(s)
      M. Venkateswar Reddy, Yasuteru Mawatari, Yuka Yajima, Kohki Satoh, S. Venkata Mohan, Young-Cheol Chang
    • Journal Title

      Bioresource Technology

      Volume: なし Pages: 155-162

    • DOI

      10.1016/j.biortech.2016.03.025

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Degradation of various toxic compounds at low and medium temperature conditions using the isolated fungus2016

    • Author(s)
      Young-Cheol Chang, Soichiro Fuzisawa, M. Venkateswar Reddy, Hikari Kobayashi, Eri Yoshida, Yuka Yajima, Tamotsu Hoshino, DuBok Choi
    • Journal Title

      CLEAN-Soil, Air, Water

      Volume: なし

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Antihyperglycemic and antioxidant activities of polysaccharide produced from Pleurotus ferulae in streptozotocin-induced diabetic rats2016

    • Author(s)
      Dubok Choi, YuLan Piao, Sun-Jong Yu, Yeon-Woong Lee, Dong-Hoon Lim, Young-Cheol Chang, Sang-Shin Park, Myung-Koo Lee, Wol-Suk Cha, Don-Sang You, Hoon Cho
    • Journal Title

      Korean J. Chem. Eng.

      Volume: なし Issue: 6 Pages: 1872-1882

    • DOI

      10.1007/s11814-016-0007-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Degradation and conversion of toxic compounds into useful bioplastics by Cupriavidus sp. CY-1: Relative expression of PhaC gene under phenol and nitrogen stress2015

    • Author(s)
      Motakatla Venkateswer Reddy , Yuka Yajima, Yasuteru Mawatari, Tamotsu Hoshino, Young-Cheol Chang
    • Journal Title

      Green Chemistry

      Volume: 17 Issue: 9 Pages: 4560-4569

    • DOI

      10.1039/c5gc01156f

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Bio-augmentation of Cupriavidus sp. CY-1 into 2,4-D contaminated soil: microbial community analysis by culture dependent and independent techniques2015

    • Author(s)
      Young-Cheol Chang, M. Venkateswar Reddy, Honoka Umemoto, Yuki Sato, Mi-Hye Kang, Yuka Yajima, Shintaro Kikuchi
    • Journal Title

      PLoS ONE

      Volume: なし Issue: 12 Pages: e0145057-e0145057

    • DOI

      10.1371/journal.pone.0145057

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 南極由来の真菌Penicillium sp. CHY-2 株による4-ブチルフェノールの低温分解及びTween 80の効果に関する研究2015

    • Author(s)
      藤澤宗一郎, 小林 ひかり, 吉田 愛里, 大平 勇一, M. Venkateswar Reddy, 張傛喆, 菊池 慎太郎
    • Journal Title

      土木工学会論文集G(環境)

      Volume: 71 Pages: 405-411

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Aquamicrobium sp. SK-2株由来Biphenyl分解酵素BphCの精製と分解特性2015

    • Author(s)
      菅原 豪人, 小山 大貴, 澤田 研, 張傛喆, 菊池 慎太郎
    • Journal Title

      土木工学会論文集G(環境)

      Volume: 71 Pages: 413-419

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Production of medium chain fatty acids using unused resources for preventing neurodegenerative disease2017

    • Author(s)
      Young-Cheol Chang, Shinji Matsuzaki, M. Venkateswar Reddy, Yuka Yajima, Tatsuaki Tsuruyama
    • Organizer
      The 8th Forum on Studies of the Environmental and Public Health Issues in the Asian Mega-cities
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of medium chain fatty acids (MCFA) using acetate and mixed culture2017

    • Author(s)
      M. Venkateswar REDDY, Yuka YAJIMA, Young-Cheol CHANG
    • Organizer
      Water and Environment Technology Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Short chain and medium chain fatty acids production using enriched mixed microbial consortia2016

    • Author(s)
      M. Venkateswar Reddy and Young-Cheol Chang
    • Organizer
      Asia-Pacific conference on Biotechnology for Waste Conversion2016
    • Place of Presentation
      Baptist University, Hong Kong, China
    • Year and Date
      2016-12-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Polyhydroxyalkanoates (PHA) production using various substrates and bacterial strains2016

    • Author(s)
      M. Venkateswar Reddy and Young-Cheol Chang
    • Organizer
      15th International Symposium Biopolymers
    • Place of Presentation
      Hotel Rafael Atocha, Madrid, Spain
    • Year and Date
      2016-09-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biodegradation of aromatic compounds using Pseudomonas palleronii and Pseudomonas pseudoflava2016

    • Author(s)
      M. Venkateswar Reddy, Erika Nakaya, Yuka Yajima, Young-Cheol Chang
    • Organizer
      7th Forum on Studies of the Environmental and Public Health Issues in the Asian Mega-cities
    • Place of Presentation
      Muroran Institute of Technology, Muroran,Hokkaido, Japan
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of Cupriavidus sp. CY-1 and its bio-augmentation into 2,4-D contaminated soil2016

    • Author(s)
      1)Honoka Umemoto, M. Venkateswar Reddy, Yuka Yajima, Young-Cheol Chang
    • Organizer
      JSED2016
    • Place of Presentation
      Muroran, Japan
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biodegradation of 4-butylphenol at low temperature conditions using the isolated fungi2016

    • Author(s)
      2)Soichiro Fuzisawa, Yuka Yajima, Young-Cheol Chang
    • Organizer
      JSED2016
    • Place of Presentation
      Muroran, Japan
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Purification and characteristics of a Biphenyl-degrading enzyme BphC from strain SK-22016

    • Author(s)
      3)Hideto Sugawara, Tatsuya Koike, Yuka Yajima, Ken Sawada, Young-Cheol Chang
    • Organizer
      JSED2016
    • Place of Presentation
      Muroran, Japan
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metabolic control of benzaldehyde oxidoreduction reaction by Pseudomonas putida2016

    • Author(s)
      4)Ryohei Yanagawa, Young-Cheol Chang, Koji Uwai and Shintaro Kikuchi
    • Organizer
      JSED2016
    • Place of Presentation
      Muroran, Japan
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 南極由来の真菌Penicillium sp. CHY-2 株による4-ブチルフェノールの低温分解及びTween 80の効果に関する研究2015

    • Author(s)
      2)藤澤宗一郎, 小林 ひかり, 吉田 愛里, 大平 勇一, M. Venkateswar Reddy, 張傛喆, 菊池 慎太郎
    • Organizer
      第52回環境工学フォーラム
    • Place of Presentation
      福島県, 鹿児島市
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Aquamicrobium sp. SK-2株由来Biphenyl分解酵素BphCの精製と分解特性2015

    • Author(s)
      3)菅原 豪人, 小山 大貴, 澤田 研, 張傛喆, 菊池 慎太郎
    • Organizer
      第52回環境工学フォーラム
    • Place of Presentation
      福島県, 鹿児島市
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] solation and characterization of Cupriavidus sp. CY-1 and its bio-augmentation into 2,4-D contaminated soil: microbial community analysis by culture dependent and independent techniques2015

    • Author(s)
      5)Young-Cheol Chang, Honoka Umemoto, Soichiro Fuzisawa, Hideto Sugawara, M. Venkateswar Reddy, Yuka Yajima, Shintaro Kikuchi
    • Organizer
      EPAM2015
    • Place of Presentation
      Xiamen, China
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Polyhydroxybutyrate (PHB) production with Cupriavidus sp. CY-1 using various toxic compounds: PhaC gene expression analysis under stress conditions2015

    • Author(s)
      6)M. Venkateswar Reddy, Young-Cheol Chang
    • Organizer
      EPAM2015
    • Place of Presentation
      Xiamen, China
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アルキルフェノール及び多環芳香族炭化水素を原料としたBacillus sp. CYR1株によるポリヒドロキシブチレート(PHB)の産生2015

    • Author(s)
      1)モタカトラ カテスワーレディ, 矢島 由佳, 張傛喆
    • Organizer
      第67回日本生物工学大会
    • Place of Presentation
      鹿児島県, 鹿児島市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Concepts of Natural Type River-Rehabilitation and Outlook of River Ecosystem Restoration2015

    • Author(s)
      Young-Cheol Chang
    • Organizer
      International Conference: Communications for Safe Cities
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2015-10-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 室蘭工業大学 研究者データベース

    • URL

      http://rdsoran.muroran-it.ac.jp/html/100000221_ja.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 微生物工学研究室

    • URL

      http://www.mmm.muroran-it.ac.jp/~ychang

    • Related Report
      2015 Annual Research Report
  • [Remarks] 研究者データベース

    • URL

      http://rd-soran.muroran-it.ac.jp/profile/ja.2GDBVy94mRvrKNMaBJdBnQ==.html

    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 中鎖脂肪酸の製造方法2016

    • Inventor(s)
      チャン ヨンチョル、矢島由佳、MV Reddy
    • Industrial Property Rights Holder
      チャン ヨンチョル、矢島由佳、MV Reddy
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-12-16
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-11-26   Modified: 2024-03-26  

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