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1神経細胞における機能的ジーンサイレンシングの可視化

Research Project

Project/Area Number 15F15711
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Molecular biology
Research InstitutionKyoto University

Principal Investigator

影山 龍一郎  京都大学, ウイルス・再生医科学研究所, 教授 (80224369)

Co-Investigator(Kenkyū-buntansha) GOLDIE BELINDA  京都大学, ウイルス・再生医科学研究所, 外国人特別研究員
Project Period (FY) 2015-07-29 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2016: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2015: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsNeurobiology / RNA dynamics / synaptic plasticity / learning and memory
Outline of Annual Research Achievements

The difficulties with defining a successful imaging protocol for the cell line used in this project have eventually been overcome and, although this took a little longer than expected, we have been able to proceed quite rapidly with the imaging experiments. The fellow has a manuscript in progress that will be able to completed after her return to Australia. As there is only have 1 month remaining on this fellowship, the fellow is reaching the end of experimental work here in Japan. The current status of her research is as follows.
1) Submission of manuscript for a project begun in Australia and completed in Japan with new technique established in the host laboratory during year 1 of the fellowship. 2) Refinement of a protocol for confocal imaging of the human neuronal cell line being used for the current project. 3) Learned new Reprogramming techniques for analysing m6A RNA modification by next-generation sequencing data. 4) Learned protein analysis techniques that have revealed unexpected and exciting information about the translation control pathways used in response to neuronal activity. 5) Co-author on a manuscript about to be submitted by the host lab. 6) Manuscript in progress from own project.

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (4 results)

All 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] University of Newcastle(Australia)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Distinct miRNA expression in dorsal striatal subregions is associated with risk for addiction in rats2015

    • Author(s)
      Quinn RK, Brown AL, Goldie BJ, Levi EM, Dickson PW, Smith DW, Cairns MJ, Dayas CV
    • Journal Title

      Transl Psychiatry

      Volume: 5 Issue: 2 Pages: e503-e503

    • DOI

      10.1038/tp.2014.144

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Activity-dependent dynamics of mRNA expression and m6A methylation in human neuronal cells2016

    • Author(s)
      Belinda J Goldie, Youqi Liao, Takayuki Ohara, Dan Ohtan Wang
    • Organizer
      International RNA Conference
    • Place of Presentation
      京都
    • Year and Date
      2016-06-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Conservative miRNA target analysis: are we limiting our discoveries of human neuronal function?2015

    • Author(s)
      Belinda J Goldie, Dan Ohtan Wang, Murray J Cairns
    • Organizer
      International Mammalian Genome Conference
    • Place of Presentation
      横浜市開港記念会館(神奈川県・横浜市中区本町)
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-11-26   Modified: 2024-03-26  

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