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Selective mating, reproductive isolation and genetic divergence in foraminifera: understanding the nature and origin of protist species

Research Project

Project/Area Number 15F15781
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Stratigraphy/Paleontology
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

土屋 正史 (2016-2017)  国立研究開発法人海洋研究開発機構, 海洋生物多様性研究分野, 技術研究員 (00435835)

北里 洋 (2015)  国立研究開発法人海洋研究開発機構, 東日本海洋生態系変動解析プロジェクトチーム, プロジェクト長 (00115445)

Co-Investigator(Kenkyū-buntansha) WEINER AGNES  国立研究開発法人海洋研究開発機構, 海洋生物多様性研究分野, 外国人特別研究員
Project Period (FY) 2015-11-09 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2017: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2016: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2015: ¥500,000 (Direct Cost: ¥500,000)
Keywords海洋原生生物 / 有孔虫 / 集団遺伝学 / 隠蔽種 / 分子系統学 / 生殖的隔離 / 分子生物地理 / 分類学 / 選択的細胞質融合 / 遺伝的変異 / 地理的隔離 / 生物地理 / 原生生物 / 種
Outline of Annual Research Achievements

本研究では,おもに岩礁地性底生有孔虫Glabratella属について,遺伝的集団の形成機構が他の有孔虫と共通するメカニズムで生じるかどうか,原生生物における種の概念を考慮に入れて検証することを目的とした。研究では,Planoglabratella opercularisを含むGlabratella属有孔虫を用いて,同所的に存在し,遺伝的分化後の時間経過がそれほど大きくない2つの遺伝的集団に遺伝的な交流が起こりうるのか,どのような条件で遺伝的な交流が起こるのか,交雑が新たな遺伝的集団の形成に寄与するのかを検証した。Planoglabratella opercularisの遺伝的集団の関係と有孔虫における「種」:自然個体群で得られた遺伝的変異および既往研究で得られた交配集団と形態型,遺伝的な変異から,有孔虫を例とした原生生物における「種」を念頭に,交配集団・非交配集団間の殻の形態的な特徴や生態を検討し,交配可能性を持つ集団との相関を検討した。P. opercularisは,地理的にも生殖的にも隔離するために,地域集団において遺伝的変異が蓄積してきたことが示されていた。本研究の解析の結果,本種には,これまで明らかにされていなかった遺伝子配列を持つ複数の地域集団が存在することが示された。これは,交配実験から明らかにされた輪状種様の集団構造を支持するものであり,さらに,地域集団の規模を識別できる重要な証拠であると言える。有孔虫においても,未分化の遺伝的な集団は,交雑により新たな遺伝的集団が形成しうるという,新たな遺伝的集団の形成メカニズムを示すことができた。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (14 results)

All 2017 2016 Other

All Int'l Joint Research (6 results) Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (4 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] ジュネーブ大学(スイス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Bremen/GEOMAR(Germany)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] JAMSTEC/Kochi University(Japan)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Geneva(Switzerland)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Edinburgh/University of St. Andrews(United Kingdom)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Lyon/CNRS/Sorbonne University(France)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Syringammina limosa sp. nov., the first xenophyophore from the deep Sea of Okhotsk: morphological and genetic description2017

    • Author(s)
      Voltski Ivan、Weiner Agnes K.M.、Tsuchiya Masashi、Kitazato Hiroshi
    • Journal Title

      Deep Sea Research Part II: Topical Studies in Oceanography

      Volume: in press Pages: 32-46

    • DOI

      10.1016/j.dsr2.2017.12.001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Caught in the act: Anatomy of an ongoing benthic-planktonic transition in a marine protist2017

    • Author(s)
      Kucera M, Silye L, Weiner AKM, Darling KF, Luebben B, Holzmann M, Pawlowski J, Schoenfeld J, Morard R
    • Journal Title

      Journal of Plankton Research

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Methodology for single-cell genetic analysis of planktonic foraminifera for studies of protist ecology and evolution2016

    • Author(s)
      Weiner AKM, Morard R, Weinkauf MFG, Darling KF, Andre A, Quillevere; F, Ujiie Y, Douady CJ, de Vargas C, Kucera M
    • Journal Title

      Frontiers in Marine Science

      Volume: 3 Pages: 255-255

    • DOI

      10.3389/fmars.2016.00255

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nomenclature for the Nameless: a proposal for an integrative Molecular taxonomy of Cryptic Diversity Exemplified by Planktonic Foraminifera2016

    • Author(s)
      R.M. Morard, G. Escarguel, A. K.M. Weiner, A. Andre, C.J. Douady, C. M. Wade, K. Darling, Y. Ujiie, H.A. Seears, and F. Quillevere T.de Garidel,- Thoron, C. de Vargas and M. Kucera
    • Journal Title

      Systematic Biology

      Volume: 10 Issue: 5 Pages: 1-31

    • DOI

      10.1093/sysbio/syw031

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Genetic diversity and reproductive isolation in Foraminifera2017

    • Author(s)
      Weiner AKM, Tsuchiya M, Kitazato H
    • Organizer
      微化石リファレンスセンター研究集会
    • Place of Presentation
      山形大学,山形県山形市
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] Genetic diversity and mating behavior in foraminifera2016

    • Author(s)
      Weiner AKM, Tsuchiya M, Kitazato H
    • Organizer
      Foraminifera Workshop
    • Place of Presentation
      Eilat, Israel
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Species classification and mating in foraminifera2016

    • Author(s)
      Weiner AKM, Tsuchiya M, Toyofuku T, Kitazato H
    • Organizer
      Protist Conference
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Morphospecies, cryptic species or biological species…? Searching for the evolutionary significance of diversity in foraminifera2016

    • Author(s)
      Weiner AKM, Tsuchiya M, Toyofuku T, Kitazato H
    • Organizer
      Japan Geoscience Union Meeting
    • Place of Presentation
      幕張メッセ,千葉県千葉市
    • Year and Date
      2016-05-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2015-11-26   Modified: 2024-03-26  

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