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Fundamentals for innovative anaerobic digestion processes using electric syntrophy

Research Project

Project/Area Number 15H01753
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Design and evaluation of sustainable and environmental conscious system
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

Watanabe Kazuya  東京薬科大学, 生命科学部, 教授 (40393467)

Co-Investigator(Kenkyū-buntansha) 阿部 貴志  新潟大学, 自然科学系, 准教授 (30390628)
上野 嘉之  鹿島建設株式会社(技術研究所), 地球環境・バイオグループ, 上席研究員 (60416724)
Research Collaborator Kouzuma Atsushi  
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Fiscal Year 2018: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2017: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2016: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Keywords電気共生 / 嫌気消化 / メタン発酵 / メタゲノム / 電子伝達 / 電気活性微生物 / カーボンブラック / マグネタイト / 発電菌 / 電気合成菌 / メタン菌 / 活性炭 / ナノ材料 / バイオマス / 微生物 / 廃棄物再資源化 / バイオエネルギー / 微生物電気合成 / 微生物電気化学 / 嫌気微生物
Outline of Final Research Achievements

Methanogenic microbiomes were supplemented with conductive nanoparticles for examining their effects on electric syntrophy and methanogenesis. Stimulatory effects of iron-oxide (magnetite) nanoparticles were observed both under static and agitated conditions. Microbiome structures were analyzed under these two conditions, and it was found that Geobacter and Methanosarcina were increased in the presence of magnetite nanoparticles under static conditions, suggesting that electric syntrophy accelerated methanogenesis. On the other hand, only Methanosarcina over grew in the presence of magnetite nanoparticles under agitated conditions. Metagenomic analyses demonstrated acetoclastic methanogenesis was activated by magnetite nanoparticles.

Academic Significance and Societal Importance of the Research Achievements

嫌気消化メタン発酵プロセスはバイオマス廃棄物の処理およびバイオガス回収に広く用いられている。しかし、プロセスの効率や安定性に課題があり、性能の向上が求められている。今までに装置の形状などの検討が行われてきたが、微生物群集自体の活性を上げることは難しかった。近年、導電性微粒子を微生物群集に添加し、微生物間の還元力の移動を促進させることでメタン発酵を効率化できる可能性が示されている。しかし、培養条件などによりその効果が安定しないと言われてきた。本研究では、微粒子の種類や撹拌条件をかえてその効果を検証し、酸化鉄微粒子には今までに知られていないメタン発酵促進効果があることをつきとめた。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (42 results)

All 2019 2018 2017 2016 2015

All Journal Article (14 results) (of which Peer Reviewed: 14 results,  Open Access: 8 results,  Acknowledgement Compliant: 2 results) Presentation (27 results) (of which Int'l Joint Research: 8 results,  Invited: 3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Understanding and engineering electrochemically active bacteria for sustainable biotechnology2019

    • Author(s)
      Hirose Atsumi、Kasai Takuya、Koga Ryota、Suzuki Yusuke、Kouzuma Atsushi、Watanabe Kazuya
    • Journal Title

      Bioresources and Bioprocessing

      Volume: 6 Issue: 1 Pages: 10-10

    • DOI

      10.1186/s40643-019-0245-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Towards development of electrogenetics using electrochemically active bacteria2019

    • Author(s)
      Hirose A, Kouzuma A, Watanabe K
    • Journal Title

      Biotechnol. Adv

      Volume: In press Issue: 6 Pages: 107351-107351

    • DOI

      10.1016/j.biotechadv.2019.02.007

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of D-Lactate Dehydrogenases in the Anaerobic Growth of Shewanella oneidensis MR-1 on Sugars2018

    • Author(s)
      Kasai Takuya、Suzuki Yusuke、Kouzuma Atsushi、Watanabe Kazuya
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 85 Issue: 3

    • DOI

      10.1128/aem.02668-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrode plate-culture methods for colony isolation of exoelectrogens from anode microbiomes2018

    • Author(s)
      Ueoka N, Kouzuma A, Watanabe K
    • Journal Title

      Bioelectrochem

      Volume: 124 Pages: 1-6

    • DOI

      10.1016/j.bioelechem.2018.06.008

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete Genome Sequence of Acidithiobacillus ferridurans JCM 189812018

    • Author(s)
      Miyauchi Tomoko、Kouzuma Atsushi、Abe Takashi、Watanabe Kazuya
    • Journal Title

      Microbiology Resource Announcements

      Volume: 7 Issue: 7

    • DOI

      10.1128/mra.01028-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methylomusa anaerophila gen. nov., sp. nov., an anaerobic methanol-utilizing bacterium isolated from a microbial fuel cell2018

    • Author(s)
      Amano N, Yamamuro A, Miyahara M, Kouzuma K, Abe T, Watanabe K
    • Journal Title

      Int. J. Syst. Evol. Microbiol.

      Volume: 68 Issue: 4 Pages: 1118-1122

    • DOI

      10.1099/ijsem.0.002635

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways2018

    • Author(s)
      Hirose A, Kasai T, Aoki M, Umemura T, Watanabe K, Kouzuma A
    • Journal Title

      Nature Commun.

      Volume: 9 Issue: 1 Pages: 1083-1083

    • DOI

      10.1038/s41467-018-03416-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CpdA is involved in amino acid metabolism in Shewanella oneidensis MR-12018

    • Author(s)
      Kasai T, Kouzuma A, Watanabe K
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 82 Issue: 1 Pages: 166-172

    • DOI

      10.1080/09168451.2017.1413326

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metagenomic insights into the ecology and physiology of microbes in bioelectrochemical systems2018

    • Author(s)
      Kouzuma Atsushi、Ishii Shun'ichi、Watanabe Kazuya
    • Journal Title

      Bioresource Technology

      Volume: 255 Pages: 302-307

    • DOI

      10.1016/j.biortech.2018.01.125

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structures, Compositions, and Activities of Live Shewanella Biofilms Formed on Graphite Electrodes in Electrochemical Flow Cells2017

    • Author(s)
      Kitayama Miho、Koga Ryota、Kasai Takuya、Kouzuma Atsushi、Watanabe Kazuya
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 83 Issue: 17

    • DOI

      10.1128/aem.00903-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative evaluation of wastewater-treatment microbial fuel cells in terms of organics removal, waste-sludge production and electricity generation2017

    • Author(s)
      Asai Y, Miyahara M, kouzuma A, Watanabe K
    • Journal Title

      Bioresour. Bioprocess.

      Volume: 4 Issue: 1 Pages: 30-30

    • DOI

      10.1186/s40643-017-0163-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Non-autotrophic methanogens dominate in thermophilic anaerobic digesters2017

    • Author(s)
      Kouzuma A, Tsutsumi M, Ishii S, Ueno Y, Abe T, Watanabe K
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1510-1510

    • DOI

      10.1038/s41598-017-01752-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Electricity generation from rice bran in microbial fuel cells2016

    • Author(s)
      Takahashi S, Miyahara M, Kouzuma A, Watanabe K
    • Journal Title

      Bioresources and Bioprocessing

      Volume: 3 Issue: 1 Pages: 50-50

    • DOI

      10.1186/s40643-016-0129-1

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microbial interspecies interactions: recent findings in syntrophic consortia2015

    • Author(s)
      Kouzuma A, Kato S, Watanabe K
    • Journal Title

      Front. Microbiol.

      Volume: 6 Pages: 477-477

    • DOI

      10.3389/fmicb.2015.00477

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Shewanella oneidensis</I>のバイオフィルム形成と電流生成を制御するECFシグマ因子の同定2019

    • Author(s)
      古賀亮太、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 海洋底泥中で複合有機物分解に関与する発電菌の同定2018

    • Author(s)
      猪鼻淑乃、高妻篤史、渡邉一哉
    • Organizer
      環境バイオテクノロジー学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 動物の腸内に電気化学活性微生物は存在するか2018

    • Author(s)
      猪鼻淑乃、高妻篤史、渡邉一哉
    • Organizer
      第17回微生物研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 発電する微生物2018

    • Author(s)
      渡邊一哉
    • Organizer
      麻酔学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] The ecology, phyiology and genetics of electrochemically active bacteria (EAB)2018

    • Author(s)
      Kazuya Watanabe
    • Organizer
      59th Annual Conference of Association of Microbiologists of India
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 電気化学活性を持つ微生物の基礎と応用2018

    • Author(s)
      渡邉一哉
    • Organizer
      日本材料学会腐食防食部門委員会例会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ニトロゲナーゼを高発現する鉄酸化細菌を用いた微生物電気化学的アンモニア生産2018

    • Author(s)
      山田祥平、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Acidithiobacillus ferrooxidansにおける窒素固定制御転写因子NifAの機能解析2017

    • Author(s)
      山田祥平、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会 2017年度大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 微生物電気化学法による空気中の窒素からのアンモニアの生産2017

    • Author(s)
      滝沢宏次、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会 2017年度大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 導電性カーボンナノ粒子カーボンブラックがメタン発酵に与える影響の解析2017

    • Author(s)
      藤縄和輝、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会 2017年度大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規電極プレート培養法を用いた水田土壌中の発電菌の単離2017

    • Author(s)
      上岡永佳、高妻篤史、渡邉一哉
    • Organizer
      環境微生物系学会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Isolation of exoelectrogens from rice paddy-field soil by using a novel electrode plate-culture method2017

    • Author(s)
      Nagayoshi UEOKA, Atsushi KOUZUMA, Kazuya WATANABE
    • Organizer
      International Meeting of The International Society for Microbial Electrochemistry and Technology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Conductive carbon nanomaterials inhibit methanogens2017

    • Author(s)
      Kazuki FUJINAWA, Atsushi KOUZUMA1, Yoshiyuki UENO2, Kazuya WATANABE
    • Organizer
      International Meeting of The International Society for Microbial Electrochemistry and Technology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The ecology and evolution of methanogens in anaerobic digesters2017

    • Author(s)
      Kazuya WATANABE
    • Organizer
      Annual Meeting on Microbial Ecology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Bioelectrochemical production of ammonia from atmospheric dinitrogen2017

    • Author(s)
      Shohei Yamada, Koji Takizawa, Atsushi Kouzuma, and Kazuya Watanabe
    • Organizer
      The 16th International Symposium on Advanced Technology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 嫌気消化槽における混合栄養的メタン生成菌の優占化2017

    • Author(s)
      藤縄和輝、高妻篤史、渡邉一哉
    • Organizer
      微生物研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 導電性ナノ粒子によるメタン発酵の高効率化2017

    • Author(s)
      稲葉涼、高妻篤史、渡邉一哉
    • Organizer
      微生物研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 活性炭微粒子の糠漬け生態系への影響2017

    • Author(s)
      垣内彩実、鈴木祐介、稲葉涼、高妻篤史、渡邉一哉
    • Organizer
      微生物研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Shewanella oneidensis MR-1株における異化代謝系遺伝子のcAMP依存的転写応答2016

    • Author(s)
      笠井 拓哉、高妻 篤史、渡邉 一哉
    • Organizer
      日本農芸化学会2016年大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] メチオニン添加によるMR-1株の電流生成量の上昇2016

    • Author(s)
      茂木 久恵 , 吉野 恵里加 , 高妻 篤史 , 渡邉 一哉
    • Organizer
      日本農芸化学会2016年大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 有機物感受性を示すAcidithiobacillus属新奇単離株の単離と生理学的性質の解析2016

    • Author(s)
      上岡永佳、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会2016年大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Shewanella oneidensis MR-1株の異化代謝系遺伝子の電位応答機構2016

    • Author(s)
      廣瀬篤弥、高妻篤史、渡邉一哉
    • Organizer
      日本農芸化学会2016年大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular mechanisms for sensing and responding to electrode potentials in Shewanella oneidensis MR-12015

    • Author(s)
      Atsumi Hirose, Atsushi Kouzuma, Kazuya Watanabe
    • Organizer
      BioMicroWorld
    • Place of Presentation
      Univ. Barcelona
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Morphological characteristics of Shewanella biofilms formed on graphite electrodes in bioelectrochemical systems2015

    • Author(s)
      Miho Kitayama, Atsushi Kouzuma, Kazuya Watanabe
    • Organizer
      BioMicroWorld
    • Place of Presentation
      Univ. Barcelona
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genomic features of uncultured methylotrophs in activated-sludge microbiomes grown under different enrichment procedures2015

    • Author(s)
      Kazuki Fujinawa, Yusuke Asai, Morio Miyahara, Atsushi Kouzuma, Kazuya Watanabe
    • Organizer
      日本微生物生態学会2015年年会
    • Place of Presentation
      霞ヶ浦亀城プラザ
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Morphological characteristics of Shewanella biofilms formed on graphite electrodes in bioelectrochemical systems2015

    • Author(s)
      Miho Kitayama
    • Organizer
      日本微生物生態学会2015年年会
    • Place of Presentation
      霞ヶ浦亀城プラザ
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular mechanisms for sensing and responding to electrode potentials in Shewanella oneidensis MR-12015

    • Author(s)
      Atsumi Hirose
    • Organizer
      日本微生物生態学会2015年年会
    • Place of Presentation
      霞ヶ浦亀城プラザ
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 微生物を用いたアンモニア製造法2017

    • Inventor(s)
      渡邉一哉、高妻篤史、滝沢宏次、山田祥平
    • Industrial Property Rights Holder
      渡邉一哉、高妻篤史、滝沢宏次、山田祥平
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-033404
    • Filing Date
      2017-02-24
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2020-03-30  

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