| Budget Amount *help |
¥38,610,000 (Direct Cost: ¥29,700,000、Indirect Cost: ¥8,910,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2017: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2015: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Outline of Final Research Achievements |
We developed the fabrication methods of topographical surfaces for cell culture, and tensile stress loading methods for cultured cell, then integrated those methods. We successfully fabricated topographical surfaces with check patterns having from 200nm to 1000nm in interval size, and fabricated conical, pyramid, triangle and semicircle patterns in micrometer order by using multi-photon 3D modeling system. Then, we developed real-time position control methods so that cultured cells on topographical surfaces can be captured in microscopic fields even under cyclic tensile stress loading, by developing the control methods for regulating strains made by stepping motors and for positioning the X-Y stage.
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