• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of X-ray single molecular instrumentation of intrinsically disordered protein in laboratory

Research Project

Project/Area Number 15H02040
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Quantum beam science
Research InstitutionThe University of Tokyo

Principal Investigator

SASAKI YUJI  東京大学, 大学院新領域創成科学研究科, 教授 (30344401)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥42,250,000 (Direct Cost: ¥32,500,000、Indirect Cost: ¥9,750,000)
Fiscal Year 2017: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2016: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2015: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
KeywordsX線 / 放射光 / 1分子 / 分子動態 / 天然変性タンパク質 / 時分割計測技術 / 自己相関 / ブリンキング現象 / 1分子 / 動態計測 / バイオテクノロジー / X線1分子追跡法 / 1分子動態 / ラボX線光源
Outline of Final Research Achievements

Diffracted X-ray Tracking (DXT) is a method of labeling a protein of interest with nanocrystals and observing the angular change of the X-ray diffraction spot (Laue method) from nanocrystals, microsecond High time-resolution, high spatial resolution of nanometer can capture the internal motions of single protein molecule. As a result of this research on this advanced original measurement method, we can evaluate the movement speed of the diffraction spot by measuring the autocorrelation and the intensity fluctuation of the diffraction intensity using monochromatic X-ray as this research result as a laboratory X-ray source. In this diffractive single molecule measurement method using monochromatic X-rays, we have found a clear blinking (X-ray blinking) of diffracted X-ray intensity.

Academic Significance and Societal Importance of the Research Achievements

社会問題となっている認知症に関連する注目タンパク質1分子の動態挙動の極めて微妙な変化を早期診断システムとして、実験室レベルの施設を用いて評価することのできる診断技術の基盤構築に成功したことは極めてその意義は大きいと考えている。社会実装に向けて、すでに数社から装置市販化のお誘いを受けている。しっかり、進めていきたい。

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (8 results)

All 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] SOLEIL(フランス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Synchrotron SOLEIL.(France)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Diffracted X-ray Blinking Tracks Single Protein Motions2018

    • Author(s)
      H.Sekiguchi et al.
    • Journal Title

      Scientific Reports

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Gold Nanocrystals as 3D High-precision Motion Tracker2018

    • Author(s)
      Yuji C. SASAKI et al.
    • Organizer
      Gold2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NEW X-RAY SINGLE MOLECULAR METHODOLOGY USING A NORMAL MONOCHROMATIC X-RAY2017

    • Author(s)
      Yuji C. Sasaki, K. Ikezaki, N Ohta, H. Sekiguchi
    • Organizer
      Annual Meeting of Biophysical Society
    • Place of Presentation
      ニューオリンズ(米国)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] X-ray Single Molecule Observations using Laboratory Monochromatic X-ray Source2016

    • Author(s)
      Yuji C. SASAKI
    • Organizer
      SOLIEL HelioBiology scientific seminar
    • Place of Presentation
      パリ(フランス)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Book] 不凍タンパク質の機能と応用(一部分担)2018

    • Author(s)
      佐々木裕次
    • Publisher
      シーエムシー出版
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 運動計測装置2016

    • Inventor(s)
      佐々木裕次
    • Industrial Property Rights Holder
      佐々木裕次
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-226495
    • Filing Date
      2016-11-22
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi