Studies on functional, hydrophobic, anti-freezing and anti-frost surfaces by using ice-growth inhibition polypeptide and a temperature response material

• Project/Area Number: 15H02220
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thermal engineering
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: HAGIWARA Yoshimichi 京都工芸繊維大学, 機械工学系, 教授 (50144332)
• Co-Investigator(Kenkyū-buntansha): 田中 直毅 京都工芸繊維大学, 分子化学系, 教授 (60243127) ; 大久保 英敏 玉川大学, 工学部, 教授 (80152081) ; 灘 浩樹 国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (90357682) ; 和久 友則 京都工芸繊維大学, 分子化学系, 助教 (30548699)
• Research Collaborator: MIURA Hitoshi
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥38,740,000 (Direct Cost: ¥29,800,000、Indirect Cost: ¥8,940,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2016: ¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000); Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: ポリペプチド / 機能表面 / 着氷防止 / 熱工学 / 機能面 / 冷凍・空調 / 凍結

Outline of Final Research Achievements

This study aims at the developing of new functional surfaces for anti-freezing and anti-frost. The anti-icing and deicing were examined. The measurement results show the new functional surfaces are promising. It is considered the reason that many aggregates of the polypeptides were adhered. We also observed frost layers on the micro-grooved surfaces. The delay in the generation and growth of the frost layers was observed.
In addition, we conducted numerical simulation on the deposited droplets on a cooling surface. The computational results showed the real phenomena could be predicted.

Academic Significance and Societal Importance of the Research Achievements

透明な低温面に水滴が付着して凍結する現象は、鉄道や自動車の窓の視認性の低下、交通信号機や監視カメラの視認性の低下、ソーラーパネルの効率低下などのトラブルの原因となる。本研究で開発した機能表面は、これらのトラブルの解決に寄与できる。また、従来の研究で検討されなかった耐久性と透明性の検討も行い、開発した機能表面の有効性が明らかになった。

Research Products

• [Int'l Joint Research] Institute for Marine & Antarctic Studies/University of Tasmania(オーストラリア)
• [Int'l Joint Research] Institute for Marine & Antarctic Studies/University of Tasmania(Australia)
• [Journal Article] Effects of winter flounder antifreeze protein on the growth of ice particles in an ice slurry flow in mini-channels (2019)
  • Author(s): Y. Takeshita, T. Waku, P. W. Wilson Y. Hagiwara
  • Journal Title: Biomolecules Volume: 9 Issue: 2 Pages: 1-12
  • DOI: 10.3390/biom9020070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Shock growth of ice crystal near equilibrium melting pressure under dynamic compression (2019)
  • Author(s): Y.-J. Kim, Y.-H. Lee, S. Lee, H. Nada, G. W. Lee
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 116 Issue: 18 Pages: 8679-8684
  • DOI: 10.1073/pnas.1818122116
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Morphological transformation of peptide nanoassemblies through conformational transition of core-forming peptides (2019)
  • Author(s): Waku, T., Hirata, N., Nozaki, M., Nogami, K.,
  • Journal Title: Polymers Volume: 1 Issue: 1 Pages: 39-39
  • DOI: 10.3390/polym11010039
  • Peer Reviewed / Open Access
• [Journal Article] Asiatic Acid, Corosolic Acid, and Maslinic Acid Interfere with Intracellular Trafficking and <i>N</i>-Linked Glycosylation of Intercellular Adhesion Molecule-1 (2018)
  • Author(s): Baba K., Hiramatsu R., Suradej B., Tanigaki R., Koeda S., Waku T., Kataoka T.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 41 Issue: 12 Pages: 1757-1768
  • DOI: 10.1248/bpb.b18-00276
  • NAID: 130007539554
  • ISSN: 0918-6158, 1347-5215
  • Year and Date: 2018-12-01
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of short-time preheating on ice growth in antifreeze polypeptides solutions in a narrow space (2018)
  • Author(s): T. Miyamoto, N. Nishi, T. Waku, N. Tanaka and Y. Hagiwara
  • Journal Title: Heat and Mass Transfer Volume: 54 Issue: 8 Pages: 2415-2424
  • DOI: 10.1007/s00231-018-2315-5
  • Peer Reviewed
• [Journal Article] Molecular dynamics analysis of synergistic effects of ions and winter flounder antifreeze protein adjacent to ice-solution surfaces (2018)
  • Author(s): T. Yasui, T. Kaijima, K. Nishio and Y. Hagiwara
  • Journal Title: Crystals Volume: 8 Pages: 1-18
  • Peer Reviewed / Open Access
• [Journal Article] 狭い空間における不凍タンパク質あるいは不凍ポリペプチド水溶液の特異な凍結面 (2018)
  • Author(s): 3萩原 良道
  • Journal Title: 日本結晶学会誌 Volume: 45 Pages: 1-10
  • NAID: 130007430036
  • Peer Reviewed
• [Journal Article] Suppression of droplets freezing on glass surfaces on which antifreeze polypeptides are adhered by a silane coupling agent (2018)
  • Author(s): K. Koshio, K. Arai, T. Waku, P. W. Wilson and Y. Hagiwara,
  • Journal Title: PLOS ONE Volume: 13 Issue: 10 Pages: 1-15
  • DOI: 10.1371/journal.pone.0204686
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Preparation of Nanoparticles Composed of Egg White Protein and their Application for Cell Adhesion Control (2018)
  • Author(s): 和久 友則, 金丸 佳央理, 廣山 慶是, 佐々木 るりほ, 森本 直也, 田中 直毅
  • Journal Title: KOBUNSHI RONBUNSHU Volume: 75 Issue: 1 Pages: 61-68
  • DOI: 10.1295/koron.2017-0070
  • NAID: 130006320133
  • ISSN: 0386-2186, 1881-5685
  • Peer Reviewed
• [Journal Article] Ice growth and interface oscillation of water droplets impinged on a cooling surface (2017)
  • Author(s): 1Y. Hagiwara, S. Ishikawa, R. Kimura and K. Toyohara
  • Journal Title: J. Crystal Growth Volume: 468 Pages: 46-53
  • DOI: 10.1016/j.jcrysgro.2016.12.095
  • Peer Reviewed
• [Journal Article] Recent advances in nanofibrous assemblies based on β-sheet-forming peptides for biomedical applications (2017)
  • Author(s): Tomonori Waku and Naok Tanaka
  • Journal Title: Polymer International Volume: 66 Pages: 277-288
  • Peer Reviewed
• [Journal Article] Ice growth inhibition in antifreeze polypeptide solution by short-time solution preheating (2016)
  • Author(s): Naoto Nishi, Takuya Miyamoto, Tomonori Waku, Naoki Tanaka and Yoshimichi Hagiwara
  • Journal Title: PLOS ONE Volume: １１ Issue: 5 Pages: 1-15
  • DOI: 10.1371/journal.pone.0154782
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of preheating on ice growth in antifreeze polypeptides solutions in a narrow space (2016)
  • Author(s): Takuya Miyamoto, Naoto Nishi, Tomonori Waku, Naok Tanaka and Yoshimichi Hagiwara,
  • Journal Title: Journal of Physics Conference Series Volume: 745 Pages: 1-8
  • DOI: 10.1088/1742-6596/745/3/032129
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Anisotropy in geometrically rough structure of ice prismatic plane interface during growth: development of a modified six-site model of H2O and a molecular dynamics simulation (2016)
  • Author(s): Hiroki Nada
  • Journal Title: J. Chem. Phys. Volume: 145 Issue: 24 Pages: 1-11
  • DOI: 10.1063/1.4973000
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] フェーズフィールド法を用いた水流中の二酸化炭素気泡の数値シミュレーション (2016)
  • Author(s): 2木下 輝彦，木村 亮太，萩原 良道
  • Journal Title: 日本混相流学会誌 混相流 Volume: 29 Pages: 533-541
  • NAID: 130005145063
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Frost layer growth on glass or copper surfaces with micro-scale lattice-patterned grooves (2019)
  • Author(s): Haruka Agui
  • Organizer: Gordon Research Conference micro and nanoscale phase change heat transfer
  • Int'l Joint Research
• [Presentation] Inhibitions of ice layer growth of droplets and frost layer formation by micro-grooved glass surfaces coated with a silane coupling agent (2019)
  • Author(s): Yoshimichi Hagiwara
  • Organizer: Gordon Research Conference micro and nanoscale phase change heat transfer
  • Int'l Joint Research
• [Presentation] 微細加工ガラス面へ衝突する水滴の凍結 (2019)
  • Author(s): 萩原 良道
  • Organizer: 第55回日本伝熱シンポジウム
• [Presentation] Effect of cooling surface micro-machining shape on frosting phenomenon on vertical plate (2018)
  • Author(s): H. Agui
  • Organizer: 29th International Symposium on Transport Phenomena
  • Int'l Joint Research
• [Presentation] 霜層厚さに及ぼす冷却面表面のぬれ性の影響 (2018)
  • Author(s): 安喰春華
  • Organizer: 2018年度日本冷凍空調学会年次大会
• [Presentation] 不凍効果を有するポリペプチドの固定方法と氷の付着力低減に関する研究 (2018)
  • Author(s): 小塩 和弥
  • Organizer: 熱工学コンファレンス2018
• [Presentation] 高分子を用いて不凍ポリペプチドを固定したガラス面における水滴凍結実験 (2018)
  • Author(s): 笠原 和也
  • Organizer: 熱工学コンファレンス2018
• [Presentation] メタダイナミクス法による結晶成長メカニズムの解析Ⅱ (2018)
  • Author(s): 灘 浩樹
  • Organizer: 第４７回結晶成長国内会議
• [Presentation] フェーズフィールド法を用いた凍結水滴の体積増加の予測 (2018)
  • Author(s): 藤井 聖明
  • Organizer: 第32回数値流体力学シンポジウム
• [Presentation] Ice growth and air/water interface motion of water droplets impinged on a horizontal cooling surface (2018)
  • Author(s): Yoshimichi Hagiwara
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice
  • Int'l Joint Research
• [Presentation] Freezing of sessile droplets on glass surfaces coated with antifreeze polypeptides by a silane coupling agent (2018)
  • Author(s): Yoshimichi Hagiwara
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice
  • Int'l Joint Research
• [Presentation] Frosts on cooling glass surfaces with micro-scale lattice-patterned grooves (2018)
  • Author(s): Yoshimichi Hagiwara
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice
  • Int'l Joint Research
• [Presentation] Molecular dynamic simulation on the interaction between ‘antifreeze’ polypeptide, water and an ice (2018)
  • Author(s): Tadashi Kaijima
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice
  • Int'l Joint Research
• [Presentation] 電場下における不凍タンパク質の氷結晶成長への影響抑制に関する分子動力学解析 (2018)
  • Author(s): 安田 昇平
  • Organizer: 日本機械学会関西支部第93期定時総会講演会
• [Presentation] Peculiar freezing fronts of solutions for antifreeze protein or antifreeze polypeptide in a narrow space (2017)
  • Author(s): Yoshimichi Hagiwara
  • Organizer: Int. workshop on new developments in step dynamics on crystal surfaces
  • Int'l Joint Research
• [Presentation] 不凍効果のあるポリペプチドを固定したガラス面の氷の付着に関する測定 (2017)
  • Author(s): 小塩 和弥
  • Organizer: 第54回日本伝熱シンポジウム
• [Presentation] 冷却面に衝突して凍結する水滴の氷成長と熱流動 (2017)
  • Author(s): 豊原 一将
  • Organizer: 第30回計算力学講演会
• [Presentation] 冷却面への衝突気流により運ばれた水滴の衝突・凍結に関する実験的研究 (2017)
  • Author(s): 米澤 翔
  • Organizer: 2017年度日本冷凍空調学会年次大会
• [Presentation] 微細溝加工面を利用した着霜の低減化 (2017)
  • Author(s): 安喰 春華
  • Organizer: 2017年度日本冷凍空調学会年次大会
• [Presentation] 不凍ポリペプチドを固定したガラス面上の水滴の凍結 (2017)
  • Author(s): 小塩 和弥
  • Organizer: 第5回生物の優れた機能から着想を得た新しいものづくりシンポジウム
• [Presentation] メタダイナミクス法による結晶成長メカニズムの解析 (2017)
  • Author(s): 灘 浩樹
  • Organizer: 第４６回結晶成長国内会議
• [Presentation] 微小領域における不凍タンパク質，不凍ポリペプチドおよびイオンを含む水溶液の凝固点と氷粒子に関する実験的研究 (2017)
  • Author(s): 谷 祥宇
  • Organizer: 第7回潜熱工学シンポジウム
• [Presentation] 微細加工面上での霜結晶の生成・成長 (2017)
  • Author(s): 安喰 春華
  • Organizer: 第7回潜熱工学シンポジウム
• [Presentation] Molecular dynamics analysis on the effects of antifreeze protein and ions on water molecules near ice layers (2017)
  • Author(s): T. Yasui, T. Kaijima and Y. Hagiwara
  • Organizer: 6th International Symposium on Micro and Nano Technology
  • Place of Presentation: 福岡市九州大学
  • Int'l Joint Research
• [Presentation] Icephobic surfaces produced with an antifreeze polypeptide and a silane coupling agent (2017)
  • Author(s): K. Arai, N. Nishi and Y. Hagiwara
  • Organizer: 6th International Symposium on Micro and Nano Technology
  • Place of Presentation: 福岡市九州大学
  • Int'l Joint Research
• [Presentation] Effects of antifreeze protein on the growth of ice particles in ice slurry flow in a mini channel (2017)
  • Author(s): Y. Takeshita and Y. Hagiwara
  • Organizer: 6th International Symposium on Micro and Nano Technology
  • Place of Presentation: 福岡市九州大学
  • Int'l Joint Research
• [Presentation] Effects of preheated or pH-controlled solution of antifreeze protein on unidirectional freezing in a narrow space (2017)
  • Author(s): T. Miyamoto, N. Nishi, T. Waku, N. Tanaka and Y. Hagiwara
  • Organizer: 6th International Symposium on Micro and Nano Technology
  • Place of Presentation: 福岡市九州大学
  • Int'l Joint Research
• [Presentation] フェーズフィールド法を用いた低温面における液滴の凍結シミュレーション (2016)
  • Author(s): 木村 亮太
  • Organizer: 日本機械学会関西支部第91期定時総会講演会
  • Place of Presentation: 寝屋川市 大阪電気通信大学
  • Year and Date: 2016-03-11
• [Presentation] 冬ガレイ由来不凍タンパク質の一部を基にしたポリペプチドの氷成長抑制に関する分子動力学解析 (2016)
  • Author(s): 寺井 航
  • Organizer: 日本機械学会関西支部第91期定時総会講演会
  • Place of Presentation: 寝屋川市 大阪電気通信大学
  • Year and Date: 2016-03-11
• [Presentation] The solidification of water droplets impinging on a cooling horizontal plate (2016)
  • Author(s): R. Kimura and Y. Hagiwara
  • Organizer: 9th International Conference on Multiphase Flow
  • Place of Presentation: イタリアフィレンツェ市Firenze Fiera
  • Int'l Joint Research
• [Presentation] Effects of preheating on ice growth in antifreeze polypeptides solutions in a narrow space (2016)
  • Author(s): T. Miyamoto, N. Nishi, T. Waku, N. Tanaka and Y. Hagiwara
  • Organizer: 7th European Thermal Science Conference
  • Place of Presentation: ポーランドクラクフ市Jagiellonian University
  • Int'l Joint Research
• [Presentation] Ice growth and interface oscillation of water droplets impinged on a cooling surface (2016)
  • Author(s): Y. Hagiwara, S. Ishikawa, R. Kimura, K. Toyohara
  • Organizer: 18th Int. Conference of Crystal Growth and Epitaxy
  • Place of Presentation: 名古屋市名古屋国際会議場
  • Int'l Joint Research
• [Presentation] 水平冷却面上の水滴の凍結に関する数値シミュレーション (2016)
  • Author(s): 萩原 良道
  • Organizer: 着氷研究会
  • Place of Presentation: 吹田市関西大学
• [Presentation] 冷却面に衝突した水滴の界面変動に対する凝固の影響 (2016)
  • Author(s): 石川 将次，福島 一孝，木村 亮太，萩原 良道
  • Organizer: 混相流シンポジウム
  • Place of Presentation: 京都市同志社大学
• [Presentation] 二種類のポリペプチド水溶液の氷表面温度と円偏光二色性の計測 (2016)
  • Author(s): 磯 大斉，宮本 拓弥，萩原 良道
  • Organizer: 日本機械学会熱工学コンファレンス
  • Place of Presentation: 松山市愛媛大学
• [Presentation] 不凍ポリペプチドとシランカップリング剤を用いた氷成長を抑制する機能面の創製 (2016)
  • Author(s): 新井 一秀，西 真人，萩原 良道
  • Organizer: 日本機械学会熱工学コンファレンス
  • Place of Presentation: 松山市愛媛大学
• [Presentation] 分子動力学解析を用いた冬ガレイ由来不凍タンパク質とイオンが有する水分子の運動抑制効果 (2016)
  • Author(s): 安井 達也，貝島 正，萩原 良道
  • Organizer: 日本機械学会熱工学コンファレンス
  • Place of Presentation: 松山市愛媛大学
• [Presentation] 不凍タンパク質の予熱と凝集による氷成長抑制の向上 (2016)
  • Author(s): 宮本 拓弥，西 真人，萩原 良道
  • Organizer: 日本機械学会熱工学コンファレンス
  • Place of Presentation: 松山市愛媛大学
• [Presentation] 冷却面に衝突する水滴の相界面変動に関する数値シミュレーション (2016)
  • Author(s): 豊原 一将，木村 亮太，萩原 良道
  • Organizer: 日本機械学会流体工学部門講演会
  • Place of Presentation: 宇部市山口大学
• [Presentation] フェーズフィールド法を用いた氷粒子を含むダクト内水流の二次元数値シミュレーション (2016)
  • Author(s): 藤井 聖明，豊原 一将，萩原 良道
  • Organizer: 第6回潜熱工学シンポジウム
  • Place of Presentation: 京都市京都工芸繊維大学
• [Presentation] 微細流路内氷スラリー流における氷粒子成長への不凍タンパク質添加の影響 (2015)
  • Author(s): 武下 雄気
  • Organizer: 第5回潜熱工学シンポジウム
  • Place of Presentation: 金沢市 金沢大学
  • Year and Date: 2015-11-26
• [Presentation] 不凍タンパク質を基にしたポリペプチドを固定した面における氷成長の抑制 (2015)
  • Author(s): 新井 一秀
  • Organizer: 日本機械学会熱工学コンファレンス
  • Place of Presentation: 吹田市 大阪大学
  • Year and Date: 2015-10-24
• [Presentation] 微細流路内における氷スラリー流の氷粒子へのポリペプチド添加の影響 (2015)
  • Author(s): 石川 将次
  • Organizer: 日本機械学会熱工学コンファレンス
  • Place of Presentation: 吹田市 大阪大学
  • Year and Date: 2015-10-24
• [Presentation] Numerical simulation of rising carbon-dioxide bubbles in downward water flow (2015)
  • Author(s): Yoshimichi Hagiwara
  • Organizer: 7th European-Japanese Two-phase Flow Meeting
  • Place of Presentation: Hotel Beau Site, Zermatt, Switzerland
  • Year and Date: 2015-10-14
  • Int'l Joint Research
• [Presentation] フェーズフィールド法を用いた水流中の二酸化炭素気泡の数値シミュレーション (2015)
  • Author(s): 萩原 良道
  • Organizer: 日本混相流学会混相流シンポジウム2015
  • Place of Presentation: 高知県土佐山田町 高知工科大学
  • Year and Date: 2015-08-06
• [Presentation] 不凍タンパク質による氷結晶抑制とその応用の可能性 (2015)
  • Author(s): 萩原 良道
  • Organizer: 公益社団法人高分子学会15-1バイオミメティクス研究会
  • Place of Presentation: 産総研、東京都
  • Year and Date: 2015-07-07
  • Invited
• [Presentation] 基質認識配列を置換したナノファイバー化シャペロンペプチドによる培養細胞の分化制御 (2015)
  • Author(s): 豊島 明
  • Organizer: 第64回 高分子学会 年次大会
  • Place of Presentation: 札幌市 札幌コンベンションセンター
  • Year and Date: 2015-05-27
• [Book] 「Bio-inspired slippery and ice-repellent coatings - fast growing fields in materials science」、生物の優れた機能から着想を得た新しいものづくり - バイオミメティクスからの発展 -、第1編第2章 (2018)
  • Author(s): Peter W. Wilson, Yoshimichi Hagiwara
  • Total Pages: 10
  • Publisher: シーエムシー出版
• [Book] 「冬カレイ由来の不凍タンパク質の代替物質であるポリペプチドを用いた着氷を抑制する機能表面」、生物の優れた機能から着想を得た新しいものづくり - バイオミメティクスからの発展 -、第3編第2章 (2018)
  • Author(s): 小塩 和哉，萩原 良道
  • Total Pages: 9
  • Publisher: シーエムシー出版
• [Book] 「不凍タンパク質とイオンの協同効果による氷結晶成長制御」、不凍タンパク質の機能と応用、第１編基礎、第６章 (2018)
  • Author(s): 萩原 良道
  • Total Pages: 8
  • Publisher: シーエムシー出版
• [Book] 「Molecular dynamics analysis of synergistic effects of ions and winter flounder antifreeze protein adjacent to ice-solution surfaces」、Advances in Computer Simulation Studies on Crystal Growth (2018)
  • Author(s): T. Yasui, T. Kaijima, K. Nishio and Y. Hagiwara
  • Total Pages: 18
  • Publisher: MDPI
  • ISBN: 9783038973577
• [Book] 潜熱蓄熱・化学蓄熱・潜熱輸送の最前線 第Ⅴ編 潜熱輸送 (2016)
  • Author(s): 萩原 良道
  • Total Pages: 8
  • Publisher: 株式会社シーエムシー出版
• [Book] Multiphase Flow Handbook 2nd ed., Section 16.3 Bio-mimetics and bio-inspiration (2016)
  • Author(s): Yoshimichi Hagiwara
  • Total Pages: 6
  • Publisher: CRC Press,
• [Remarks] 京都工芸繊維大学輸送現象制御学研究室
  • URL: http://www.cis.kit.ac.jp/~kitagawa/