| Budget Amount *help |
¥43,420,000 (Direct Cost: ¥33,400,000、Indirect Cost: ¥10,020,000)
Fiscal Year 2017: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2016: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2015: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
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| Outline of Final Research Achievements |
We performed slice cultures of the adult brain of Hes1 reporter mice and found that Hes1 is steadily expressed at high levels in quiescent neural stem cells. It is likely that this steady Hes1 expression may repress Mash1 expression. Because Mash1-negative neural stem cells do not proliferate well, we next tried to induce Mash1 oscillations optogenetically. The Mash1-inducible system was introduced with lentivirus into neural stem cells in the adult mouse brain, and oscillatory expression was induced by light illumination. After one week, we found that many neural stem cells were activated and produced new neurons.
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