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Building new foundation for biological sciences based on transposon-mediated gene trapping

Research Project

Project/Area Number 15H02370
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Genome biology
Research InstitutionNational Institute of Genetics

Principal Investigator

Kawakami Koichi  国立遺伝学研究所, 個体遺伝研究系, 教授 (70195048)

Co-Investigator(Renkei-kenkyūsha) ISHITANI Tohru  群馬大学, 生体調節研究所, 教授 (40448428)
ITOH Motoyuki  千葉大学, 大学院薬学研究院, 教授 (20377906)
INAGAKI Naoyuki  奈良先端大, バイオサイエンス研究科, 准教授 (20223216)
OGURA Toshihiko  東北大学, 加齢医学研究所, 教授 (60273851)
KAWAKAMI Atsushi  東工大, 生命理工学研究科, 准教授 (00221896)
SEHARA Atsuko  京都大学, 再生医科学研究所, 教授 (60209038)
TAMURA Koji  東北大学, 生命科学研究科, 教授 (70261550)
HIGASHIJIMA Shinichi  自然科学研究機構, 生命創成探求センター, 教授 (80270479)
HIRATA Hiromi  青山学院大学, 理工学部, 教授 (60402450)
YAMASU Kyo  埼玉大学, 理工学研究科, 教授 (60230439)
YAMAMOTO Naoyuki  名古屋大学, 生命農学研究科, 教授 (80256974)
HIBI Masahiko  名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
FUJITA Misato  神奈川大学, 理学部, 助教 (60633550)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000)
Fiscal Year 2017: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2016: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2015: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Keywordsゲノム / 遺伝子 / 遺伝学 / 神経科学 / 発生・分化 / ゼブラフィッシュ / 遺伝子トラップ / 脳・神経 / 器官形成
Outline of Final Research Achievements

(1) We performed a gene trap screen using the Tol2 transposon system and identified a large number of transgenic fish expressing Gal4FF specifically in various cells, tissues and organs. We analyzed the transgenic fish by Southern blot and PCR, and mapped the transposon insertions on the zebrafish genome.
(2) We conducted collaborative research with experts of the studies of developmental biology and organogenesis based on the transgenic fish lines expressing Gal4FF. The results were published as 40 articles written in English.
(3) We succeeded in developing a system using the botulinum toxin gene that can efficiently inhibit the function of Gal4FF expressing neurons. By performing calcium imaging, we discovered a neural circuit that connects a visual stimulus with the inferior lobe of the hypothalamus, which is the center of appetite. We succeeded in identifying a neuronal population in the telencephalon, that is essential for fear conditioning.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (79 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (15 results) Journal Article (40 results) (of which Int'l Joint Research: 27 results,  Peer Reviewed: 38 results,  Open Access: 34 results,  Acknowledgement Compliant: 5 results) Presentation (20 results) (of which Int'l Joint Research: 16 results,  Invited: 17 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] National Institute of Health/Salk Institute for Biological Studies(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of the Sunshine Coast/The University of Queensland(オーストラリア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] UCL School of Pharmacy/University of Edinburgh(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Hubrecht Institute(オランダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Friedrich-Schiller-University(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ICM/Universite Paris/INSERM(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Hong Kong Baptist University/Chinese Academy of Sciences(中国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] King’s College London/Oxford University/The Francis Crick Institute(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] NTNU(ノルウェー)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Ludwig-Maximilians University/DZNE/Max Planck Institute(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Institut du Cerveau/CNRS UMR 7622/Institut Curie(France)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Sydney(Australia)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Helmholtz Zentrum(Germany)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Structure/Function Studies of the α4 Subunit Reveal Evolutionary Loss of a GlyR Subtype Involved in Startle and Escape Responses2018

    • Author(s)
      Leacock, S., Syed, P., James, V.M., Bode, A., Kawakami, K., Keramidas, A., Suster, M., Lynch, J.W., and Harvey, R.J.
    • Journal Title

      Frontiers in Molecular Neuroscience

      Volume: 11 Pages: 23-23

    • DOI

      10.3389/fnmol.2018.00023

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair2018

    • Author(s)
      Lopez-Baez, J.C., Simpson, D.J., Forero, L.L., Zeng, Z., Brunsdon, H., Salzano, A., Brombin, A., Wyatt, C., Rybski, W., Huitema, L.FA., Dale, R.M., Kawakami, K., Englert, C., Chandra, T., Schulte-Merker, S., Hastie, N.D., and Patton, E.E.
    • Journal Title

      eLife

      Volume: 7

    • DOI

      10.7554/elife.30657

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish.2018

    • Author(s)
      Lal P, Tanabe H, Suster ML, Ailani D, Kotani Y, Muto A, Itoh M, Iwasaki M, Wada H, Yaksi E, Kawakami K.
    • Journal Title

      BMC Biol.

      Volume: 16 Issue: 1 Pages: 45-45

    • DOI

      10.1186/s12915-018-0502-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Activation of the hypothalamic feeding centre upon visual prey detection2017

    • Author(s)
      Muto, A., Lal, P., Ailani, D., Abe, G., Itoh, M., and Kawakami, K.
    • Journal Title

      Nature Communications

      Volume: 8 Issue: 1 Pages: 15029-15029

    • DOI

      10.1038/ncomms15029

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A novel perivascular cell population in the zebrafish brain2017

    • Author(s)
      Galanternik, M.V., Castranova, D., Gore, A.V., Blewett, N.H., Jung, H.M., Stratton, A.N., Kirby, M.R., Iben, J., Miller, M.F., Kawakami, K., Maraia, R.J., and Weinstein, B.M.
    • Journal Title

      eLife

      Volume: 6

    • DOI

      10.7554/elife.24369

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analysis of transcription factors expressed at the anterior mouse limb bud2017

    • Author(s)
      Yokoyama Shigetoshi、Furukawa Soichi、Kitada Shoya、Mori Masaki、Saito Takeshi、Kawakami Koichi、Belmonte Juan Carlos Izpisua、Kawakami Yasuhiko、Ito Yoshiaki、Sato Tempei、Asahara Hiroshi
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 5 Pages: e0175673-e0175673

    • DOI

      10.1371/journal.pone.0175673

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Activin-A enhances mTOR signaling to promote aberrant chondrogenesis in fibrodysplasia ossificans progressiva.2017

    • Author(s)
      Hino K, Horigome K, Nishio M, Komura S, Nagata S, Zhao C, Jin Y, Kawakami K, Yamada Y, Ohta A, Toguchida J, Ikeya M.
    • Journal Title

      J Clin Invest

      Volume: 127 Issue: 9 Pages: 3339-3352

    • DOI

      10.1172/jci93521

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Preface to Vertebrate Brains: evolution, structures and functions2017

    • Author(s)
      Kawakami, K., and Murakami, Y.
    • Journal Title

      Development, Growth and Differentiation

      Volume: 59 Issue: 4 Pages: 160-162

    • DOI

      10.1111/dgd.12375

    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Journal Article] Transposons as tools for functional genomics in vertebrate models2017

    • Author(s)
      Kawakami, K., Largaespada, D.A., and Ivics, Z.
    • Journal Title

      Trends in Genetics

      Volume: 33 Issue: 11 Pages: 7840801-7840801

    • DOI

      10.1016/j.tig.2017.07.006

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Granule cells control recovery from classical conditioned fear responses in the zebrafish cerebellum2017

    • Author(s)
      Matsuda, K., Yoshida, M., Kawakami, K., Hibi, M., and Shimizu. T.
    • Journal Title

      Scientific reports

      Volume: 7 Issue: 1 Pages: 11865-11865

    • DOI

      10.1038/s41598-017-10794-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel zebrafish ret heterozygous model of Hirschsprung disease identifies a functional role for mapk10 as a modifier of enteric nervous system phenotype severity2017

    • Author(s)
      Heanue, T.A., Boesmans, W., Bell, D.M., Kawakami, K., Vanden Berghe, P., and Pachnis, V.
    • Journal Title

      PLoS Genetics

      Volume: 12 Issue: 11 Pages: e1006439-e1006439

    • DOI

      10.1371/journal.pgen.1006439

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Motile-cilia-mediated flow improves sensitivity and temporal resolution of olfactory computations2017

    • Author(s)
      Reiten, I., Emre Uslu, F., Fore, S., Pelgrims, R., Ringers, C., Diaz Verdugo, C., Hoffman, M., Lal, P., Kawakami, K., Pekkan, K., Yaksi, E., and Jurisch-Yaksi, N.
    • Journal Title

      Current Biology

      Volume: 27 Issue: 2 Pages: 166-174

    • DOI

      10.1016/j.cub.2016.11.036

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Left habenula mediates light-preference behavior in zebrafish via an asymmetrical visual pathway2017

    • Author(s)
      Bai-bing, Z., Yao, Y.-y., Zhang, H.-f., Kawakami, K., and Du, J.-l.
    • Journal Title

      Neuron

      Volume: 93 Issue: 4 Pages: 914-928

    • DOI

      10.1016/j.neuron.2017.01.011

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration.2017

    • Author(s)
      Yu Ohki, Andrea Wenninger-Weinzierl, Alexander Hruscha, Kazuhide Asakawa, Koichi Kawakami, Christian Haass, Dieter Edbauer and Bettina Schmid
    • Journal Title

      Molecular Neurodegeneration

      Volume: 12 Issue: 1 Pages: 6-6

    • DOI

      10.1186/s13024-016-0146-8

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Proteolysis regulates cardiomyocyte maturation and tissue integration2017

    • Author(s)
      Fukuda, R., Gunawan, F., Beisaw, A., Jimenez-Amilburu, V., Maischein, H.-M., Kostin, S., Kawakami, K., and Stainier, D.Y.R.
    • Journal Title

      Nature Communications

      Volume: 8 Issue: 1 Pages: 14495-14495

    • DOI

      10.1038/ncomms14495

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold2017

    • Author(s)
      Tomoya Hasegawa, Christopher J. Hall, Philip S. Crosier, Gembu Abe, Koichi Kawakami, Akira Kudo, *Atsushi Kawakami
    • Journal Title

      eLife

      Volume: 10 Pages: 7554-7554

    • DOI

      10.7554/elife.22716

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Triclosan (TCS) exposure impairs lipid metabolism in zebrafish embryos2016

    • Author(s)
      Ho, J.C.H., Hsiao, C.D., Kawakami, K., and William, K.F.
    • Journal Title

      Aquatic Toxicology

      Volume: 173 Pages: 29-35

    • DOI

      10.1016/j.aquatox.2016.01.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cellular dynamics of regeneration reveals role of two distinct Pax7 stem cell populations in larval zebrafish muscle repair2016

    • Author(s)
      Pipalia, T.G., Koth, J., Roy, S.D., Hammond, C.L., Kawakami, K., and Hughes, S.M.
    • Journal Title

      Disease Models and Mechanisms

      Volume: 9 Issue: 6 Pages: 671-684

    • DOI

      10.1242/dmm.022251

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gal4 Driver Transgenic Zebrafish: Powerful Tools to StudyDevelopmental Biology, Organogenesis, and Neuroscience2016

    • Author(s)
      Kawakami K, Asakawa K, Hibi M, Itoh M, Muto A, Wada H.
    • Journal Title

      Adv Genet.

      Volume: 95 Pages: 65-87

    • DOI

      10.1016/bs.adgen.2016.04.002

    • ISBN
      9780128048009
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zebrafish lines expressing UAS-driven red probes for monitoring cytoskeletal dynamics2016

    • Author(s)
      Takamasa Mizoguchi, Koichi Kawakami, Motoyuki Itoh
    • Journal Title

      genesis

      Volume: 54(9) Issue: 9 Pages: 483-489

    • DOI

      10.1002/dvg.22955

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo.2016

    • Author(s)
      Kamezaki A, Sato F, Aoki K, Asakawa K, Kawakami K, Matsuzaki F, Sehara-Fujiwara A.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 28873-28873

    • DOI

      10.1038/srep28873

    • NAID

      120005819488

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fgf signalling controls diverse aspects of fin regeneration2016

    • Author(s)
      Shibata, E., Yokota, Y., Horita, N., Kudo, A., Abe, G., Kawakami, K. and *Kawakami, A.
    • Journal Title

      Development

      Volume: 143 Issue: 16 Pages: 2920-2929

    • DOI

      10.1242/dev.140699

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Optimization of a Neurotoxin to Investigate the Contribution of Excitatory Interneurons to Speed Modulation In Vivo2016

    • Author(s)
      Jenna R. Sternberg, Kristen E. Severi, Kevin Fidelin, Johanna Gomez, Hideshi Ihara, Yara Alcheikh, Jeffrey M. Hubbard, Koichi Kawakami, Maximiliano Suster, Claire Wyatt
    • Journal Title

      Current Biology

      Volume: 17 Issue: 17 Pages: 2319-2328

    • DOI

      10.1016/j.cub.2016.06.037

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Stereotyped initiation of retinal waves by bipolar cells via presynaptic NMDA autoreceptors2016

    • Author(s)
      Zhang, R.-w., Li, X.-q., Kawakami, K., and Du, J.-l.
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 12650-12650

    • DOI

      10.1038/ncomms12650

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Calcium dysregulation contributes to neurodegeneration in FTLD patient iPSC-derived neurons2016

    • Author(s)
      Imamura, K., Sahara, N., Kanaan, N. M., Tsukita, K., Kondo, T., Kutoku, Y., Ohsawa, Y., Sunada, Y., Kawakami, K., Hotta, A., Yawata, S., Watanabe, D., Hasegawa, M., Trojanowski, J. Q., Lee, V. M.-Y., Suhara, T., Higuchi, M., and Inoue, H.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 34904-34904

    • DOI

      10.1038/srep34904

    • NAID

      120005850272

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP-Positive Cells from Transgenic Zebrafish Lines2016

    • Author(s)
      Tanabe H and Kawakami K et al.
    • Journal Title

      Methods in Molecular Biology

      Volume: 1451 Pages: 93-106

    • DOI

      10.1007/978-1-4939-3771-4_7

    • ISBN
      9781493937691, 9781493937714
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Calcium imaging of neuronal activity in free-swimming larval zebrafish2016

    • Author(s)
      Muto, A., and Kawakami, K.
    • Journal Title

      Methods in Molecular Biology

      Volume: 1451 Pages: 333-341

    • DOI

      10.1007/978-1-4939-3771-4_23

    • ISBN
      9781493937691, 9781493937714
    • Related Report
      2016 Annual Research Report
  • [Journal Article] Tol2-mediated transgenesis, gene trapping, enhancer trapping, and Gal4-UAS system2016

    • Author(s)
      Kawakami K, Asakawa K, Muto A, Wada H.
    • Journal Title

      Methods Cell Biol.

      Volume: 135 Pages: 19-37

    • DOI

      10.1016/bs.mcb.2016.01.011

    • ISBN
      9780128034743
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes.2016

    • Author(s)
      Oka M, Mura S, Yamada K, Sangel P, Hirata S, Maehara K, Kawakami K, Tachibana T, Ohkawa Y, Kimura H, Yoneda Y
    • Journal Title

      eLIFE

      Volume: 5

    • DOI

      10.7554/elife.09540

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CSF-contacting neurons regulate locomotion by relaying mechanical stimuli to spinal circuits2016

    • Author(s)
      Bohm, U.L., Prendergast, A., Djenoune, L., Figueiredo, S.N., Gomez, J., Stokes, C., Kaiser, S., Suster, M., Kawakami, K., Charpentier, M., Concordet, J.-P., Rio, J.-P., Del Bene, F., and Wyart, C.
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 10866-10866

    • DOI

      10.1038/ncomms10866

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neuregulin 1 type II-ErbB signaling promotes cell divisions generating neurons from neural progenitor cells in the developing zebrafish brain2015

    • Author(s)
      Sato T, Sato F, Kamezaki A, Sakaguchi K, Tanigome R, Kawakami K, *Sehara-Fujisawa A
    • Journal Title

      PLOS ONE

      Volume: 10(5) Issue: 5 Pages: e0127360-e0127360

    • DOI

      10.1371/journal.pone.0127360

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish2015

    • Author(s)
      Xiao Y, Faucherre A, Pola-Morell L, Heddleston J, Liu T, Chew T, Sato F, Sehara-Fujisawa A, Kawakami K, Lopez-Schier H
    • Journal Title

      Disease Models & Mechanisms

      Volume: 8 Issue: 6 Pages: 553-64

    • DOI

      10.1242/dmm.018184

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-32015

    • Author(s)
      Auer, T.O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J.-P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H., and Del Bene, F.
    • Journal Title

      eLife

      Volume: 4

    • DOI

      10.7554/elife.05061

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Deubiquitinating enzymes regulate Hes1 stability and neuronal differentiation.2015

    • Author(s)
      Taeko Kobayashi, Yumiko Iwamoto, Kazuhiro Takashima, Akihiro Isomura, Yoichi Kosodo, Koichi Kawakami, Tomoki Nishioka, Kozo Kaibuchi, Ryoichiro Kageyama
    • Journal Title

      FEBS Journal

      Volume: 印刷中 Issue: 13 Pages: 2475-2487

    • DOI

      10.1111/febs.13290

    • NAID

      120005670897

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] NAS: Neuron Analyzer Suite for automatic analysis of neuronal activities from calcium imaging data.2015

    • Author(s)
      Hamed, M,, Odul, J., Miller, A.S., Kawakami, K., and Asanobu, K.
    • Journal Title

      International Journal of Pharma Medicine and Biological Sciences

      Volume: 4 Pages: 167-170

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diversification of non-visual photopigment parapinopsin in spectral sensitivity for diverse pineal functions2015

    • Author(s)
      M. Koyanagi, S. Wada, E. Kawano-Yamashita, Y. Hara, S. Kuraku, S. Kosaka, K. Kawakami, S. Tamotsu, H. Tsukamoto, Y. Shichida, A. Terakita
    • Journal Title

      BMC Biology

      Volume: 13 Issue: 1 Pages: 73-73

    • DOI

      10.1186/s12915-015-0174-9

    • NAID

      120005758203

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] BAC transgenic zebrafish reveal hypothalamic enhancer activity around obesity associated SNP rs9939609 within the human FTO gene2015

    • Author(s)
      Rinkwitz, S., Geng, F.-S., Manning, E., Suster, M., Kawakami, K., and Becker, T.S.
    • Journal Title

      Genesis

      Volume: 53 Issue: 10 Pages: 640-651

    • DOI

      10.1002/dvg.22884

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Stable and bicistronic expression of two genes in somite- and lateral plate-derived tissues to study chick limb development2015

    • Author(s)
      Bourgeois, A., de Lima, J.E., Charvet, B., Kawakami, K., Stricker, S., and Duprez, D.
    • Journal Title

      BMC Developmental Biology

      Volume: 15 Issue: 1 Pages: 39-39

    • DOI

      10.1186/s12861-015-0088-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213.2015

    • Author(s)
      Kotani Y, Morito D, Yamazaki S, Ogino K, Kawakami K, Takashima S, Hirata H, Nagata K.
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 16161-16161

    • DOI

      10.1038/srep16161

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Endothelial Ca 2+ oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo2015

    • Author(s)
      Yokota Y*, Nakajima H*(Co-first author), Wakayama Y, Muto A, Kawakami K, Fukuhara S, Mochizuki N.
    • Journal Title

      eLife

      Volume: 4

    • DOI

      10.7554/elife.08817

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] The amygdalar and hippocampal functions in zebrafish2018

    • Author(s)
      Koichi Kawakami
    • Organizer
      The 5th European Zebrafish PI Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The neuronal circuitry for prey capture in zebrafish: from visual stimuli to the hypothalamic feeding center2017

    • Author(s)
      Kawakami, K.
    • Organizer
      The 10th Zebrafish Disease Models Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Enhancer trapping and calcium imaging reveal functional neuronal circuitries essential for fish behaviors2017

    • Author(s)
      Kawakami, K.
    • Organizer
      2nd Malaysia Zebrafish Disease Model Workshop
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Enhancer trapping and calcium imaging reveal a functional neuronal circuit essential for the prey capture behavior: from visual stimuli to the hypothalamic feeding center2017

    • Author(s)
      Kawakami, K.
    • Organizer
      The 5th Zebrafish Research Conference of China
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The amygdalar and hippocampal functions in zebrafish The 2017 workshop on Zebrafish2017

    • Author(s)
      Kawakami, K.
    • Organizer
      Neural Circuits and Behavior
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The zebrafish amygdala and hippocampus2017

    • Author(s)
      Koichi Kawakami
    • Organizer
      7th Strategic Conference of Zebrafish Investigators
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The study of amygdala and hippocampal functions in zebrafish2016

    • Author(s)
      Koichi Kawakami
    • Organizer
      FishMed2016
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Activation of the hypothalamic feeding centre upon visual prey detection2016

    • Author(s)
      Akira Muto, Koichi Kawakami
    • Organizer
      The 39th Annual Meeting of the Japan Neuroscience Society
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The study of hippocampal functions in zebrafish2016

    • Author(s)
      Hideyuki Tanabe, Pradeep Lal, Akira Muto, Koichi Kawakami
    • Organizer
      第22回小型魚類研究会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ゼブラフィッシュの扁桃体、海馬に相当する機能的神経回路の遺伝学的解析2016

    • Author(s)
      川上浩一
    • Organizer
      日本進化学会 第18回東京大会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] The zebrafish amygdala and hippocampus2016

    • Author(s)
      Koichi Kawakami
    • Organizer
      The 9th Zebrafish Disease Models Conference
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The zebrafish amygdala and hippocampus2016

    • Author(s)
      Koichi Kawakami
    • Organizer
      7th Asia Oceania Zebrafish Meeting
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The zebrafish amygdala and hippocampus2016

    • Author(s)
      Koichi Kawakami
    • Organizer
      The 4th Imaging Structure and Function in the Zebrafish Brain Conference
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ゼブラフィッシュを用いた海馬機能の研究2015

    • Author(s)
      田辺 英幸
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゼブラフィッシュ研究の最前線2015

    • Author(s)
      川上浩一
    • Organizer
      第49回日本実験動物技術者協会総会
    • Place of Presentation
      静岡
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] The study of amygdala and hippocampal functions in zebrafish2015

    • Author(s)
      Koichi Kawakami
    • Organizer
      Imaging the Nervous System, Univ of Cambridge
    • Place of Presentation
      Cambridge, UK
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The study of amygdala and hippocampal functions in zebrafish2015

    • Author(s)
      Koichi Kawakami
    • Organizer
      Zebrafish Disease Models Conference
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-08-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetic dissection of the fear circuit in zebrafish2015

    • Author(s)
      Koichi Kawakami
    • Organizer
      The 38th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Kobe
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional dissection of the zebrafish telencephalon by the Gal4-UAS approach2015

    • Author(s)
      Koichi Kawakami
    • Organizer
      Circuits and Behavior in Tuscany
    • Place of Presentation
      Montecastelli, Italy
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional dissection of the zebrafish telencephalon by the Gal4-UAS approach2015

    • Author(s)
      Koichi Kawakami
    • Organizer
      The 48th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Tsukuba
    • Year and Date
      2015-06-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Zebrafish: Methods and Protocols2016

    • Author(s)
      Kawakami, K., Patton, E.E., Orger, M.
    • Total Pages
      366
    • Publisher
      Springer
    • ISBN
      9781493937714
    • Related Report
      2016 Annual Research Report
  • [Remarks] gene trap & enhancer trap transgenic zebrafish

    • URL

      http://kawakami.lab.nig.ac.jp/ztrap/

    • Related Report
      2017 Annual Research Report
  • [Remarks] zTrap

    • URL

      http://kawakami.lab.nig.ac.jp/ztrap/

    • Related Report
      2015 Annual Research Report
  • [Remarks] ZeBrain

    • URL

      http://zebrain.nig.ac.jp/zebrain/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2022-09-27  

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