| Budget Amount *help |
¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000)
Fiscal Year 2017: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2015: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
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| Outline of Final Research Achievements |
We conducted our research by focusing on how microRNAs mediate gene silencing of their target mRNAs by utilizing two distinct molecular mechanisms: "translational repression" and "poly(A) shortening". We identified a novel E3 ubiquitin ligase named "Iruka", which acts to ensure the quality of Argonaute proteins, the core factor in the microRNA pathway. Moreover, we revealed a series of new insights into the enzymatic properties and their ATP dependence of the deadenylase enzymes in the CCR4-NOT complex, which is recruited by microRNAs to mediate the poly(A) shortening of their target mRNAs.
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