| Project/Area Number |
15H02383
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山下 克美 金沢大学, 薬学系, 准教授 (10191280)
若林 雄一 千葉県がんセンター(研究所), その他部局等, 研究員 (40303119)
藤芳 暁 東京工業大学, 理工学研究科, 助教 (70371705)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2017: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2015: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
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| Keywords | テロメア / DNA複製 / 相同性DNA組換え / リボソームDNA / CST複合体 / rDNA / 複製フォーク停止 / DNA相同組換え / 遺伝的不安定性 / 遺伝学 / stn1 / 温度感受性株 / 複製フォーク / サブテロメア / 塩基除去修復 / ８オキソグアニン |
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| Outline of Final Research Achievements |
The CST complex consists of three subunits of CTC1, STN1 and TEN1. It is phylogenetically conserved among budding yeast, fission yeast, mammals and plants, but its exact function remains to be explored. Because in fission yeast, stn1 is essential, we isolated a temperature-sensitive mutant stn1-1. Culturing stn1-1 in permissive, semi-permissive or non-permissive temperatures, we found that Stn1 is required for efficient progressing of DNA replication fork at subtelomeres. When stn1 is dysfunctional, replication forks fails to complete replication to the very end of telomere, leading to massive loss of subtelomeres and telomeres in a couple of cell cycles.
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