| Budget Amount *help |
¥38,220,000 (Direct Cost: ¥29,400,000、Indirect Cost: ¥8,820,000)
Fiscal Year 2017: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2016: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2015: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Outline of Final Research Achievements |
The structural features of the membrane fusion protein (CDV-F) of canine distemper virus (CDV) were observed by cryo-electron microscopy. The sample preparation and grid manufacturing conditions were successfully optimized for high-resolution analysis. On the other hand, for the receptor-binding protein (CDV-H) of the same virus, several anti-CDV-H monoclonal antibodies were setablished by rat intestinal lymph node method. The subtypes and CDV-H binding properties of the obtained antibodies were examined. The membrane fusion assay showed that each antibody has different binding affinity and specificity. These results give insight on the molecular basis of viral entry and vaccine effectiveness of CDV and related viruses.
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