Interorgan communication governing neuronal cell growth under distinct nutrient conditions

• Project/Area Number: 15H02400
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: Uemura Tadashi 京都大学, 生命科学研究科, 教授 (80213396)
• Co-Investigator(Renkei-kenkyūsha): HATTORI Yukako 京都大学, 大学院生命科学研究科, 助教 (50646768)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥28,860,000 (Direct Cost: ¥22,200,000、Indirect Cost: ¥6,660,000); Fiscal Year 2017: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2016: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2015: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
• Keywords: 器官形成 / 栄養 / 神経細胞 / 樹状突起 / 器官間相互作用 / 全身性シグナル因子 / 脂肪組織

Outline of Final Research Achievements

We addressed how neuronal growth is controlled by nutritional status using Class IV dendritic arborization neurons in Drosophila larvae. We found that dendritic arbors of Class IV neurons became more complex when larvae were reared on a low-yeast diet, which contains lower amounts of amino acids and other ingredients, compared to a high-yeast diet. We also showed that mitochondrial dysfunction triggers dendritic loss in a manner dependent on eIF2α phosphorylation and translation inhibition.

Research Products

• [Journal Article] Small conductance Ca2+-activated K+ channels induce the firing pause periods during the activation of Drosophila nociceptive neurons (2017)
  • Author(s): Koun Onodera, Shumpei Baba, Akira Murakami, Tadashi Uemura, Tadao Usui
  • Journal Title: eLife Volume: 6 Pages: 1-17
  • DOI: 10.7554/elife.29754
  • NAID: 120006358779
  • Peer Reviewed / Open Access
• [Journal Article] Mitochondrial dysfunction induces dendritic loss via eIF2α phosphorylation. (2017)
  • Author(s): Taiichi Tsuyama, Asako Tsubouch, Tadao Usui, Hiromi Imamura, and Tadashi Uemura.
  • Journal Title: The Journal of Cell Biology Volume: 216(3) Issue: 3 Pages: 815-834
  • DOI: 10.1083/jcb.201604065
  • NAID: 120006360787
  • Peer Reviewed / Open Access
• [Journal Article] Nutrient-dependent dendrite arborization of somatosensory neurons. (2016)
  • Author(s): Kaori Watanabe, Yuki Furumizo, Tadao Usui, Yukako Hattori, and Tadashi Uemura.
  • Journal Title: Genes to Cells Volume: 22(1) Issue: 1 Pages: 105-114
  • DOI: 10.1111/gtc.12451
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 個体の成長と成熟を司る栄養応答システムの解明新学術創成研究機構 (2018)
  • Author(s): 上村 匡
  • Organizer: 革新的統合バイオ研究コア 栄養・代謝研究ワークショップ「生体トランジスタシスを司る栄養応答システムの動作基盤」
  • Invited
• [Presentation] Systemic TGFβ/Activin signaling governs adaptive metabolic responses to nutrient balances of Drosophila species. (2018)
  • Author(s): 上村 匡
  • Organizer: 京都生体質量分析研究会
• [Presentation] The regulatory mechanism provoking the robust avoidance behavior by small conductance Ca2+-activated K+ channels in Drosophila larval nociceptive neurons. (2018)
  • Author(s): Koun Onodera
  • Organizer: the 16th International Student Seminar
  • Int'l Joint Research
• [Presentation] Nutrient-dependent increased dendritic arborization of somatosensory neurons in Drosophila larvae. (2018)
  • Author(s): Yasutetsu Kanaoka
  • Organizer: the 16th International Student Seminar
  • Int'l Joint Research
• [Presentation] Nutrient-dependent increased dendritic arborization of somatosensory neurons in Drosophila larvae. (2018)
  • Author(s): Yasutetsu Kanaoka
  • Organizer: CDB Symposium 2018 “Dynamic Homeostasis: from Development to Aging”
• [Presentation] Deciphering distinct dietary responses governing growth among Drosophila species by comparative multi-omics approaches. (2017)
  • Author(s): Kaori Watanabe
  • Organizer: HiHA第7回Workshop
  • Place of Presentation: 広島大学先端科学総合研究棟（東広島市）
  • Year and Date: 2017-09-23
• [Presentation] Nutri-developmental biology：栄養バランス変化に適応して成長するシステムの追究. (2017)
  • Author(s): 上村 匡
  • Organizer: HiHA第7回Workshop
  • Place of Presentation: 広島大学先端科学総合研究棟（東広島市）
  • Year and Date: 2017-09-23
  • Invited
• [Presentation] 被食者・捕食者双方の遺伝学を用いた個体成長を支える栄養基盤の追究 (2017)
  • Author(s): 服部佑佳子
  • Organizer: 第４回発生における代謝を考える会
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2017-02-20
  • Invited
• [Presentation] 栄養バランス変化に応じて成長する機構の解明に向けた種間比較解析 (2017)
  • Author(s): 渡辺佳織
  • Organizer: 第４回発生における代謝を考える会
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2017-02-20
  • Invited
• [Presentation] The molecular basis of distinct adaptive responses to nutrient balances between generalist and specialist species. (2017)
  • Author(s): Tadashi Uemura
  • Organizer: European Molecular Biology Laboratory Symposia "Metabolism in Time and Space: Emerging Links to Cellular and Developmental Programs"
  • Int'l Joint Research
• [Presentation] Nutrient-dependent increased dendritic arborization of somatosensory neurons in Drosophila larvae. (2017)
  • Author(s): Yasutetsu Kanaoka
  • Organizer: The 3rd International Insect Hormone Workshop.
• [Presentation] The molecular basis of distinct adaptive responses to nutrient balances between generalist and specialist species. (2017)
  • Author(s): Tadashi Uemura
  • Organizer: The 3rd International Insect Hormone Workshop
  • Invited
• [Presentation] Drosophila侵害覚ニューロンにおける発火パターンの制御機構～ 逃げるは恥だが役に立つ in science ～ (2017)
  • Author(s): 小野寺 孝興
  • Organizer: 第3回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
• [Presentation] The regulatory mechanism provoking the robust avoidance behavior by small conductance Ca2+-activated K+ channels in Drosophila larval nociceptive neurons. (2017)
  • Author(s): Koun Onodera
  • Organizer: The 29th CDB Meeting: Mavericks, New Models in Developmental Biology
• [Presentation] Information processing mediated by firing rate fluctuations in Drosophila sensory system. (2017)
  • Author(s): Koun Onodera
  • Organizer: Kyoto University Cell Biology, Developmental Biology, and Systems Biology Course Meeting
• [Presentation] 個体成長において栄養バランス変化への適応を支える全身性シグナル経路 Systemic signaling for adaptation to nutrient balances in animal growth. (2017)
  • Author(s): 上村 匡
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] Drosophila幼虫侵害覚ニューロンにおけるsmall conductance Ca2+依存性K+チャネルを介した制御機構により忌避行動が促進される The regulatory mechanism provoking the robust avoidance behavior by small conductance Ca2+-activated K+ channels in Drosophila larval nociceptive neurons. (2017)
  • Author(s): 小野寺 孝興
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] FRET型ATPセンサーを用いたショウジョウバエ神経細胞におけるATPの濃度と消費速度の可視化 (2017)
  • Author(s): 津山 泰一
  • Organizer: 日本生体エネルギー研究会第43回討論会
• [Presentation] The molecular basis of distinct responses to nutrient balances between generalist and specialist species栄養バランス変化に適応し成長する分子機構の解明に向けて (2016)
  • Author(s): Yukako Hattori
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] Deciphering distinct dietary responses governing growth among Drosophila species by comparative multi-omics approaches. (2016)
  • Author(s): Kaori Watanabe
  • Organizer: The 12th Japanese Drosophila Research Conference(JDRC12)
  • Place of Presentation: Rikkyo University(Tokyo)
  • Year and Date: 2016-09-11
• [Presentation] The molecular basis of distinct responses to nutrient balances between Drosophila generalist and specialist species. (2016)
  • Author(s): Yukako Hattori
  • Organizer: The 12th Japanese Drosophila Research Conference(JDRC12)
  • Place of Presentation: Rikkyo University(Tokyo)
  • Year and Date: 2016-09-11
• [Presentation] 栄養バランス変化に適応して成長する生体システムの解明に向けて (2016)
  • Author(s): 服部 佑佳子
  • Organizer: 第88回遺伝学会
  • Place of Presentation: 日本大学国際関係学部三島校舎（静岡県三島市）
  • Year and Date: 2016-09-07
  • Invited
• [Presentation] 栄養バランス依存的な個体成長システム―種間比較マルチオミックス解析― (2016)
  • Author(s): 上村 匡
  • Organizer: 生物資源ゲノム解析拠点シンポジウム・研究発表会「NGS情報をどう活かすか、基礎から応用まで」
  • Place of Presentation: 東京農業大学（東京）
  • Year and Date: 2016-09-06
• [Presentation] Neuronal processing of harmful stimuli mediated by dendritic Ca2+ rises and specific firing patterns. (2016)
  • Author(s): 小野寺 孝興
  • Organizer: 第15回京都大学・国立台湾大学・筑波大学合同「分子細胞生物学シンポジウム」(the 15th International Symposium)
  • Int'l Joint Research
• [Presentation] Comparative studies of the nocifensive behaviors of Drosophila species and the firing properties of the somatosensory neurons. (2016)
  • Author(s): 西村 理沙
  • Organizer: 第15回京都大学・国立台湾大学・筑波大学合同「分子細胞生物学シンポジウム」(the 15th International Symposium)
  • Int'l Joint Research
• [Presentation] Deciphering the molecular basis of responses to nutrient balances governing animal growth (2016)
  • Author(s): Tadashi Uemura
  • Organizer: Duke Nus Medical School NBD Invited Speaker Seminar
  • Invited
• [Presentation] 栄養バランス依存的な個体成長システム-種間比較マルチオミックス解析- (2016)
  • Author(s): 上村 匡
  • Organizer: 生物資源ゲノム解析拠点シンポジウム・研究発表会「NGS情報をどう活かすか、基礎から応用まで」
• [Presentation] Neuronal processing of harmful stimuli mediated by dendritic Ca2+ rises and specific firing patterns. (2016)
  • Author(s): Tadao Usui
  • Organizer: The 12th Japanese Drosophila Research,Conference(JDRC12)
• [Presentation] Comparative studies of the nocifensive behaviors of Drosophila species and the firing properties of the somatosensory neurons. (2016)
  • Author(s): Risa Nishimura
  • Organizer: The 12th Japanese Drosophila Research Conference(JDRC12)
• [Presentation] Nutri-developmental biology：栄養バランス変化に適応して成長するシステムの追究 (2016)
  • Author(s): 上村 匡
  • Organizer: HiHA第7回Workshop
  • Invited
• [Presentation] 痛覚ニューロンの特異的な発火パターンによる適応的な逃避行動制御メカニズムの追究 The control of adaptive escape behavior by the induction of specific firing patterns in primary somatosensory neurons (2016)
  • Author(s): 碓井理夫
  • Organizer: 第39回日本分子生物学会年会
• [Book] "The Cadherin Superfamily" Chapter 10 Seven-pass transmembrane cadherin CELSRs, and Fat4 and Dchs1 cadherins: from planar cell polarity to three-dimensional organ architecture. (2016)
  • Author(s): Dongbo Shi, Masaki Arata, Tadao Usui, Toshihiko Fujimori and Tadashi Uemura.
  • Total Pages: 25
  • Publisher: Springer Publishing Company
• [Remarks] 研究室ホームページ
  • URL: http://www.cellpattern.lif.kyoto-u.ac.jp/