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Studies on the molecular basis and evolution of Prunus-specific self-incompatibility recognition system

Research Project

Project/Area Number 15H02431
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionKyoto University

Principal Investigator

Tao Ryutaro  京都大学, 農学研究科, 教授 (10211997)

Co-Investigator(Kenkyū-buntansha) 赤木 剛士  京都大学, 農学研究科, 助教 (50611919)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2016: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2015: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Keywords果樹園芸学 / 園芸学 / 受粉受精 / 自家不和合性 / 果樹ゲノム解析 / 果樹 / 果樹ゲノム科学 / 組換え遺伝子 / 園芸 / 園芸ゲノム科学
Outline of Final Research Achievements

Genome-wide analysis of the evolutional radiation of S locus-related F-box genes were conducted to elucidate the evolutionary path to the establishment of the Prunus-specific S-RNase-based gametophytic self-incompatibility (GSI) system. The analysis indicated that the Prunus pollen S F-box gene, SFB, diverged from pollen S F-box genes of the Malinae (Rosaceae), Solanaceae, and Plantaginaceae. early after the establishment of the Eudicots. The analysis also indicated the Prunus SFB gene originated in a recent Prunus-specific gene duplication event, which could contribute to the establishment of the Prunus-specific GSI recognition mechanism. Both evolutional and biochemical analyses conducted strongly indicated that pollen expressed F-box genes (SLFLs) located close to the S locus of Prunus may be the so-called general inhibitor (GI) of the GI model explaining Prunus-specific GSI recognition mechanism.

Academic Significance and Societal Importance of the Research Achievements

本研究は,バラ科サクラ属におけるS-RNase型自家不和合性の認識機構が, S-RNase型の自家不和合性を示す他の植物の認識機構とは,大きく異なるという科学的な謎に対して,進化学的な見地から一つの答えを示した.また,本研究で行われた進化学的な研究と生化学的な研究で得た結果を総合考察し,サクラ属果樹の自家不和合性認識機構を説明するための仮説モデルのおけるジェネラルインヒビターの分子実体が,サクラ属不和合性S遺伝子座近傍に座乗する遺伝子にコードされるF-boxタンパク質であることを示唆した.これらは,サクラ属果樹の生産上の大きな障壁である自家不和合性の打破に活用できる有力な知見となる.

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (29 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (17 results) (of which Int'l Joint Research: 9 results,  Invited: 4 results) Remarks (4 results)

  • [Int'l Joint Research] CSIRO/Zaragoza 園芸試験場(スペイン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Zaragoza園芸試験場(スペイン)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Genome Re-Sequencing of Diverse Sweet Cherry (Prunus avium) Individuals Reveals a Modifier Gene Mutation Conferring Pollen-Part Self-Compatibility2018

    • Author(s)
      Ono Kentaro、Akagi Takashi、Morimoto Takuya、Wunsch Ana、Tao Ryutaro
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Issue: 6 Pages: 1265-1275

    • DOI

      10.1093/pcp/pcy068

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Insights into the Prunus-specific S-RNase-based self-incompatibility system from a genome-wide analysis of the evolutionary radiation of S locus-related F-box genes2016

    • Author(s)
      Akagi, T., I.M. Henry, T. Morimoto, and R. Tao
    • Journal Title

      Plant Cell Physiol

      Volume: 57 Issue: 6 Pages: 2905-2915

    • DOI

      10.1093/pcp/pcw077

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Recognition of a wide-range of S-RNases by S locus F-box like 2, a general-inhibitor candidate in the Prunus-specific S-RNase-based self-incompatibility system2016

    • Author(s)
      Matumoto, D. and R. Tao
    • Journal Title

      Plant Mol. Biol.

      Volume: 91 Issue: 4-5 Pages: 459-469

    • DOI

      10.1007/s11103-016-0479-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System2016

    • Author(s)
      Matsumoto, D. and R. Tao
    • Journal Title

      The Horticulture Journal

      Volume: 85 Issue: 4 Pages: 289-305

    • DOI

      10.2503/hortj.MI-IR06

    • NAID

      130005433244

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2016 Annual Research Report
    • Open Access / Acknowledgement Compliant
  • [Journal Article] Differences in Physiological Characteristics and Gene Expression Levels in Fruits between Japanese Persimmon (<i>Diospyros kaki</i> Thunb.) ‘Hiratanenashi’ and Its Small Fruit Mutant ‘Totsutanenashi’2016

    • Author(s)
      Morimoto, T., T. Akagai, and R. Tao.
    • Journal Title

      The Horticulture Journal

      Volume: 85 Issue: 4 Pages: 306-314

    • DOI

      10.2503/hortj.MI-119

    • NAID

      130005433280

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Virus-induced gene silencing in various Prunus species with the Apple latent spherical virus vector2016

    • Author(s)
      Kawai, T., A. Gonoi, M. Nitta, N. Yamagishi, N. Yoshikawa and R. Tao
    • Journal Title

      Scientia Hort.

      Volume: 199 Pages: 103-113

    • DOI

      10.1016/j.scienta.2015.12.031

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Genome re-sequencing of diverse sweet cherry (Prunus avium) individuals reveals a modifier gene mutation conferring pollen-part self-compatibility2018

    • Author(s)
      Ono, K., T. Akagi, T. Morimoto, A. Wunsch and R. Tao
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The 9th International Rosaceae Genomics Conference2018

    • Author(s)
      Tao, R.
    • Organizer
      The 9th International Rosaceae Genomics Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] New insights into the distinct S-RNase-based self-incompatibility mechanisms in Prunus2018

    • Author(s)
      Tao, R
    • Organizer
      The 30th International Horticulture Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of the pollen-part modifier gene involved in self-incompatibility reaction in sweet cherry2018

    • Author(s)
      Ono, K., T. Morimoto, T. Akagi, A. Wunsch and R. Tao
    • Organizer
      The 30th International Horticulture Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 全ゲノムシークエンス解析によるカンカオウトウの花粉側自家不和合性共通因子の探索2018

    • Author(s)
      大野健太朗・森本拓也・A. Wunsch・赤木剛士・田尾龍太郎
    • Organizer
      園芸学会平成30年度春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 全ゲノムシークエンス解析によるカンカオウトウの花粉側自家不和合性共通因子の探索2017

    • Author(s)
      大野健太朗・森本拓也・A.W&uuml;nsch・赤木剛士・田尾龍太郎
    • Organizer
      園芸学会春季大会
    • Place of Presentation
      日本大学生物資源科学部
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] バラ科サクラ属に特異な自家不和合性認識機構の進化成立過程の解析2017

    • Author(s)
      森本拓也・赤木剛士・田尾龍太郎
    • Organizer
      園芸学会春季大会
    • Place of Presentation
      日本大学生物資源科学部
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] Insights into the evolution and establishment of the Prunus-specific self-incompatibility recognition mechanism2017

    • Author(s)
      Morimoto, T., T. Akagi and R. Tao
    • Organizer
      ISHS 8th Cherry Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Whole genome sequencing approach to identify pollen-part modifier conferring self-compatibility in sweet cherry 'Clistobalina'2017

    • Author(s)
      Ono, K., T. Morimoto, T. Akagi, Ana Wunsch and R. Tao
    • Organizer
      ISHS 8th Cherry Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Recent advances in our understanding of the S-RNase-based gametophytic self-incompatibility system in Prunus2017

    • Author(s)
      Tao, R
    • Organizer
      ISHS 8th Cherry Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] サクラ属S locus F-box-likes (SLFLs)のペチュニア形質転換体における機能解析2016

    • Author(s)
      森本拓也・渡邉美佳子・田尾龍太郎
    • Organizer
      園芸学会秋季大会
    • Place of Presentation
      名城大学
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] Next generation sequencing analysis to identify modifier gene candidates conferring pollen-part self-compatibility in sweet cherry ‘Cristobalina2016

    • Author(s)
      Morimoto, T., A. W&uuml;nsch, M. Watanabe, T. Akagi, and R. Tao
    • Organizer
      The 8th International Rosaceae Genomics Conference
    • Place of Presentation
      Angers, France
    • Year and Date
      2016-06-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional characterization of Prunus SLFLs in transgenic Petunia2016

    • Author(s)
      Morimoto, T. and R. Tao
    • Organizer
      II ISHS International Workshop on Floral Biology and S-Incompatibility in Fruit Specie
    • Place of Presentation
      Murcia, Spain
    • Year and Date
      2016-05-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 全ゲノムワイド連鎖・発現解析によるカンカオウトウにおける花粉側non-S自家和合化因子の探索2016

    • Author(s)
      森本拓也・Ana Wunsch・渡邉美佳子・赤木剛士・田尾 龍太郎
    • Organizer
      園芸学会平成28年度春季大会
    • Place of Presentation
      神奈川
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] サクラ属およびリンゴ亜連植物におけるS遺伝子の進化系統解析2015

    • Author(s)
      森本拓也・赤木剛士・田尾龍太郎
    • Organizer
      園芸学会平成27年度秋季大会
    • Place of Presentation
      徳島
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] バラ科サクラ属果樹類におけるS-RNase依存性配偶体型自家不和合性elf-incompatibility in the Rosaceae2015

    • Author(s)
      田尾龍太郎
    • Organizer
      食品酵素化学研究会第15回学術講演会
    • Place of Presentation
      京都
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evolutionary dynamics of the specificity determinant genes for the S-RNase-based self-incompatibility in the Rosaceae2015

    • Author(s)
      Morimoto, T., T. Akagi and R. Tao
    • Organizer
      XIV Eucarpia Fruit breeding and genetics symposium
    • Place of Presentation
      Bologna, Italy
    • Year and Date
      2015-06-14
    • Related Report
      2015 Annual Research Report
  • [Remarks] 京都大学農学研究科果樹園芸学研究室

    • URL

      http://www.pomology.kais.kyoto-u.ac.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 京都大学農学研究科果樹園学研究室

    • URL

      http://www.pomology.kais.kyoto-u.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 京都大学大学院農学研究科農学専攻果樹園芸学研究室

    • URL

      http://www.pomology.kais.kyoto-u.ac.jp/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 京都大学アカデミックデイ2015 -果樹のライフサイクル研究の最前線-

    • URL

      http://research.kyoto-u.ac.jp/academic-day/2015/19/

    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2020-03-30  

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