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Structure-function analysis and study on reaction mechanism of microbial cysteine glycosidases

Research Project

Project/Area Number 15H02443
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

Fushinobu Shinya  東京大学, 大学院農学生命科学研究科(農学部), 教授 (00302589)

Co-Investigator(Kenkyū-buntansha) 藤田 清貴  鹿児島大学, 農水産獣医学域農学系, 准教授 (20381189)
石渡 明弘  国立研究開発法人理化学研究所, 開拓研究本部, 専任研究員 (70342748)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000)
Fiscal Year 2018: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2015: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Keywords酵素 / タンパク質 / 有機化学 / 応用微生物学 / 糖鎖 / 糖質関連酵素 / 植物病原菌 / 選択的化学合成 / 応用微生物 / 蛋白質
Outline of Final Research Achievements

We found a novel type of glycoside hydrolase (beta-L-Araf-ase HypBA1) that has the Cys nucleophile coordinated to Zn. In this study, an examination of its reaction mechanism and structural analysis of related enzymes (proteins) were performed. Novel mechanism-based inhibitors that specifically react with the Cys residue were developed. Using these inhibitors, we could reveal the three-dimensional structures of all of the reaction steps of HypBA1. We also found novel beta-L-Araf-ases and performed functional and structural analyses with related enzymes. Characteristics of the enzymes and proteins involved in the beta-arabinooligosaccharide-degradation were revealed.

Academic Significance and Societal Importance of the Research Achievements

活性中心がCysである糖質加水分解酵素としてはこれ以外に全く前例がなく新規な発見である。さらにHypBA1で反応前(酵素-基質ミカエリス型複合体)、反応中間体アナログ(ハロアセトアミド体阻害剤との複合体)、反応後(産物であるβ-L-Arafとの複合体)の全てで立体構造に成功し、反応機構を解明した。本酵素は善玉菌として有名な腸内細菌であるビフィズス菌が食品に含まれるβ-アラビノオリゴ糖分解に関わる経路で重要な役割を担っており、ヒトの健康に有効なプレバイオティクス開発に繋がると期待される。

Report

(5 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (52 results)

All 2020 2019 2018 2017 2016 2015 Other

All Journal Article (27 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 27 results,  Open Access: 9 results,  Acknowledgement Compliant: 7 results) Presentation (22 results) (of which Int'l Joint Research: 4 results,  Invited: 5 results) Remarks (3 results)

  • [Journal Article] Structural analysis of β‐L‐arabinobiose‐binding protein in the metabolic pathway of hydroxyproline‐rich glycoproteins in Bifidobacterium longum2020

    • Author(s)
      Miyake Masayuki、Terada Tohru、Shimokawa Michiko、Sugimoto Naohisa、Arakawa Takatoshi、Shimizu Kentaro、Igarashi Kiyohiko、Fujita Kiyotaka、Fushinobu Shinya
    • Journal Title

      The FEBS Journal

      Volume: in press Issue: 23 Pages: 5114-5129

    • DOI

      10.1111/febs.15315

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of β-L-arabinobiosidase belonging to glycoside hydrolase family 1212020

    • Author(s)
      Keita Saito, Alexander Holm Viborg, Shiho Sakamoto, Takatoshi Arakawa, Chihaya Yamada, Kiyotaka Fujita, Shinya Fushinobu
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 6 Pages: e0231513-e0231513

    • DOI

      10.1371/journal.pone.0231513

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural basis for broad substrate specificity of UDP-glucose 4-epimerase in the human milk oligosaccharide catabolic pathway of Bifidobacterium longum2019

    • Author(s)
      Nam Young-Woo、Nishimoto Mamoru、Arakawa Takatoshi、Kitaoka Motomitsu、Fushinobu Shinya
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 11081-11081

    • DOI

      10.1038/s41598-019-47591-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Understanding the Molecular Mechanism Underlying the High Catalytic Activity of p-Hydroxybenzoate Hydroxylase Mutants for Producing Gallic Acid2019

    • Author(s)
      Moriwaki Yoshitaka、Yato Mirai、Terada Tohru、Saito Seiji、Nukui Noriyuki、Iwasaki Takumi、Nishi Tatsunari、Kawaguchi Yuko、Okamoto Ken、Arakawa Takatoshi、Yamada Chihaya、Fushinobu Shinya、Shimizu Kentaro
    • Journal Title

      Biochemistry

      Volume: 58 Issue: 45 Pages: 4543-4558

    • DOI

      10.1021/acs.biochem.9b00443

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two novel α-L-arabinofuranosidases from Bifidobacterium longum subsp. longum belonging to glycoside hydrolase family 43 cooperatively degrade arabinan2019

    • Author(s)
      Komeno M, Hayamizu H, Fujita K, Ashida H
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 85 Issue: 6

    • DOI

      10.1128/aem.02582-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of plant arabinogalactan proteins by intestinal bacteria: characteristics and functions of the enzymes involved.2019

    • Author(s)
      Fujita, K., Sasaki, Y. & Kitahara, K.
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 103 Issue: 18 Pages: 7451-7457

    • DOI

      10.1007/s00253-019-10049-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissecting the Stereocontrolled Conversion of Short-Lived Sulfenic Acid by Lachrymatory Factor Synthase2019

    • Author(s)
      Arakawa Takatoshi、Sato Yuta、Yamada Masayuki、Takabe Jumpei、Moriwaki Yoshitaka、Masamura Noriya、Kato Masahiro、Aoyagi Morihiro、Kamoi Takahiro、Terada Tohru、Shimizu Kentaro、Tsuge Nobuaki、Imai Shinsuke、Fushinobu Shinya
    • Journal Title

      ACS Catalysis

      Volume: 10 Issue: 1 Pages: 9-19

    • DOI

      10.1021/acscatal.9b03720

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a GH36 β-L-Arabinopyranosidase in <i>Bifidobacterium adolescentis</i>2018

    • Author(s)
      Sasaki Yuki、Togo Nami、Kitahara Kanefumi、Fujita Kiyotaka
    • Journal Title

      Journal of Applied Glycoscience

      Volume: 65 Issue: 2 Pages: 23-30

    • DOI

      10.5458/jag.jag.JAG-2018_001

    • NAID

      130006742938

    • ISSN
      1344-7882, 1880-7291
    • Year and Date
      2018-05-20
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification, functional characterization, and crystal structure determination of bacterial levoglucosan dehydrogenase2018

    • Author(s)
      Sugiura Masayuki、Nakahara Moe、Yamada Chihaya、Arakawa Takatoshi、Kitaoka Motomitsu、Fushinobu Shinya
    • Journal Title

      Journal of Biological Chemistry

      Volume: 293 Issue: 45 Pages: 17375-17386

    • DOI

      10.1074/jbc.ra118.004963

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bimodal Glycosyl Donors Protected by 2-O-(ortho-Tosylamido)benzyl Group2018

    • Author(s)
      Ding Feiqing、Ishiwata Akihiro、Ito Yukishige
    • Journal Title

      Organic Letters

      Volume: 20 Issue: 14 Pages: 4384-4388

    • DOI

      10.1021/acs.orglett.8b01922

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stereodivergent Mannosylation Using 2-O-(ortho-Tosylamido)benzyl Group2018

    • Author(s)
      Ding Feiqing、Ishiwata Akihiro、Ito Yukishige
    • Journal Title

      Organic Letters

      Volume: 20 Issue: 16 Pages: 4833-4837

    • DOI

      10.1021/acs.orglett.8b01979

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins2018

    • Author(s)
      Nakamura Masayuki、Yasukawa Yuino、Furusawa Akira、Fuchiwaki Tamao、Honda Takashi、Okamura Yuta、Fujita Kiyotaka、Iwai Hisashi
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 8 Pages: e0201982-e0201982

    • DOI

      10.1371/journal.pone.0201982

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural and thermodynamic insights into β-1, 2-glucooligosaccharide capture by a solute-binding protein in Listeria innocua.2018

    • Author(s)
      Abe, K., Sunagawa, N., Terada, T., Takahashi, Y., Arakawa, T., Igarashi, K., Samejima, M., Nakai, H., Taguchi, H., Nakajima, M., and Fushinobu, S.
    • Journal Title

      J. Biol. Chem.

      Volume: 293 Issue: 23 Pages: 8812-8828

    • DOI

      10.1074/jbc.ra117.001536

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Degradative enzymes for type II arabinogalactan side chains in Bifidobacterium longum subsp. longum2018

    • Author(s)
      Fujita Kiyotaka、Sakamoto Ayami、Kaneko Satoshi、Kotake Toshihisa、Tsumuraya Yoichi、Kitahara Kanefumi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 103 Issue: 3 Pages: 1299-1310

    • DOI

      10.1007/s00253-018-9566-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Insight into Evolution of Symbiosis between Breast-Fed Infants and a Member of the Human Gut Microbiome Bifidobacterium longum2017

    • Author(s)
      Yamada C, Gotoh A, Sakanaka M, Hattie M, Stubbs KA, Katayama-Ikegami A, Hirose J, Kurihara S, Arakawa T, Kitaoka M, Okuda S, Katayama T and Fushinobu S.
    • Journal Title

      Cell Chemical Biology

      Volume: 24 Issue: 4 Pages: 515-524

    • DOI

      10.1016/j.chembiol.2017.03.012

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The First Crystal Structure of a Family 129 Glycoside Hydrolase from a Probiotic Bacterium Reveals Critical Residues and Metal Co-factors2017

    • Author(s)
      Mayo Sato, Dorothee Liebschner, Yusuke Yamada, Naohiro Matsugaki, Takatoshi Arakawa, Siobhan S. Wills, Mitchell Hattie, Keith A. Stubbs, Tasuku Ito, Toshiya Senda, Hisashi Ashida and Shinya Fushinobu
    • Journal Title

      Journal of Biological Chemistry

      Volume: 292 Issue: 29 Pages: 12126-12138

    • DOI

      10.1074/jbc.m117.777391

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Crystal Structure and Substrate Specificity Modification of Acetyl Xylan Esterase from Aspergillus luchuensis2017

    • Author(s)
      Komiya Dai、Hori Akane、Ishida Takuya、Igarashi Kiyohiko、Samejima Masahiro、Koseki Takuya、Fushinobu Shinya
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 83 Issue: 20

    • DOI

      10.1128/aem.01251-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of α-l-arabinopyranosidases from human gut microbiome expands the diversity within glycoside hydrolase family 422017

    • Author(s)
      Viborg Alexander Holm、Katayama Takane、Arakawa Takatoshi、Abou Hachem Maher、Lo Leggio Leila、Kitaoka Motomitsu、Svensson Birte、Fushinobu Shinya
    • Journal Title

      Journal of Biological Chemistry

      Volume: 292 Issue: 51 Pages: 21092-21101

    • DOI

      10.1074/jbc.m117.792598

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The crystal structure of an inverting glycoside hydrolase family 9 exo-β-D-glucosaminidase and the design of glycosynthase2016

    • Author(s)
      Y. Honda, S. Arai, K. Suzuki, M. Kitaoka, and S. Fushinobu
    • Journal Title

      Biochem. J.

      Volume: 473 Issue: 4 Pages: 463-472

    • DOI

      10.1042/bj20150966

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Archaeal Mo-containing glyceraldehyde oxidoreductase isozymes exhibit diverse substrate specificities through unique subunit assemblies2016

    • Author(s)
      T. Wakagi, H. Nishimasu, M. Miyake, and S. Fushinobu
    • Journal Title

      PLOS ONE

      Volume: 11 Issue: 1 Pages: e0147333-e0147333

    • DOI

      10.1371/journal.pone.0147333

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity and transglycosylation activity of metagenomic β-glucosidase Td2F2.2016

    • Author(s)
      Tomohiko Matsuzawa, Toshinori Jo, Taku Uchiyama, Jenny A Manninen, Takatoshi Arakawa, Kentaro Miyazaki, Shinya Fushinobu, Katsuro Yaoi
    • Journal Title

      FEBS Journal

      Volume: - Issue: 12 Pages: 2340-2353

    • DOI

      10.1111/febs.13743

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] rystal structures of archaeal 2-oxoacid:ferredoxin oxidoreductases from Sulfolobus tokodaii2016

    • Author(s)
      Z. Yan, A. Maruyama, T. Arakawa, S. Fushinobu, and T. Wakagi
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 33061-33061

    • DOI

      10.1038/srep33061

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 糖タンパク質糖鎖の加水分解酵素に関する研究2016

    • Author(s)
      藤田清貴
    • Journal Title

      応用糖質科学

      Volume: 6 Pages: 30-36

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characterization of a β-L-Arabinopyranosidase from <i>Bifidobacterium longum</i> subsp. <i>longum</i>2015

    • Author(s)
      藤田清貴ら
    • Journal Title

      Journal of Applied Glycoscience

      Volume: 62 Issue: 1 Pages: 1-6

    • DOI

      10.5458/jag.jag.JAG-2014_006

    • NAID

      130004705329

    • ISSN
      1344-7882, 1880-7291
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization and crystal structure determination of β-1,2-mannobiose phosphorylase from Listeria innocua2015

    • Author(s)
      T. Tsuda, T. Nihira, K. Chiku, E. Suzuki, T. Arakawa, M. Nishimoto, M. Kitaoka, H. Nakai, and S. Fushinobu
    • Journal Title

      FEBS Lett.

      Volume: 589 Issue: 24PartB Pages: 3816-3821

    • DOI

      10.1016/j.febslet.2015.11.034

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Crystal structure and substrate recognition of cellobionic acid phosphorylase, which plays a key role in oxidative cellulose degradation by microbes2015

    • Author(s)
      Y.-W. Nam, T. Nihira, T. Arakawa, Y. Saito, M. Kitaoka, H. Nakai, and S. Fushinobu
    • Journal Title

      J. BIol. Chem.

      Volume: 290 Issue: 30 Pages: 18281-18292

    • DOI

      10.1074/jbc.m115.664664

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] N-Glycosylation with Synthetic Undecaprenyl Pyrophosphate-Linked Oligosaccharide to Oligopeptides by PglB Oligosaccharyltransferase from Campylobacter jejuni2015

    • Author(s)
      Ishiwata A, Taguchi Y, Lee YJ, Watanabe T, Kohda D, Ito Y.
    • Journal Title

      Chembiochem

      Volume: 16 Issue: 5 Pages: 731-737

    • DOI

      10.1002/cbic.201402658

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Structural analysis of β-L-arabinofuranosidases in GH127 and GH1462019

    • Author(s)
      Shinya Fushinobu
    • Organizer
      13th Carbohydrate Bioengineering Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structural analysis for studying the reaction mechanism of β-L-arabinofuranosidases2019

    • Author(s)
      Shinya Fushinobu
    • Organizer
      ACS Fall 2019 National Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] GH127 beta-L-アラビノフラノシダーゼ阻害剤の合成検討2019

    • Author(s)
      石渡 明弘、伊藤 幸成
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] N-(L-アラビノフラノシル) ハロアセトアミド両異性体の立体選択的合成2018

    • Author(s)
      成田 覚、石渡 明弘、伊藤 幸成
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 結核菌アラビナン分解酵素の機能解析に向けたオリゴアラビノフラノシド誘導体の合成研究2018

    • Author(s)
      石渡 明弘、伊藤 幸成
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 渦鞭毛藻由来 beta-1,5 結合した-L-アラビノフラノシド三糖構造の構築と構造解析2018

    • Author(s)
      石渡 明弘、伊藤 幸成
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Synthetic studies on L-arabinofuranosylated probes for bifidobacterial beta-L-arabinofuranosidase2018

    • Author(s)
      Ishiwata, A.; Narita, S.; Kimura, K.; Fushinobu S.; Fujita K.; Ito, Y.
    • Organizer
      International Symposium on ER stress, glycosylation, homeostasis and diseases
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bifidobacterium longum由来の beta-L-アラビノフラノシダーゼの機構解析に向けたアラビノフラノース構造を有するプローブの合成検討2018

    • Author(s)
      石渡 明弘、成田 覚、藤田 清貴、伏信 進矢、伊藤 幸成
    • Organizer
      第37回日本糖質学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Strategy toward the synthesis of glycoconjugate from plant and bacteria2018

    • Author(s)
      Ishiwata, A.
    • Organizer
      ACS On Campus in AIST
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] beta-L-アラビノフラノシダーゼの機能解析に向けたアラビノフラノース構造を有する阻害剤の合成検討2018

    • Author(s)
      石渡 明弘、成田 覚、藤田 清貴、伏信 進矢、伊藤 幸成
    • Organizer
      日本応用糖質科学会平成30年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Synthesis of the arabinan fragments of mycobacterial cell wall2018

    • Author(s)
      Ishiwata, A.
    • Organizer
      9th Asian Community of Glycoscience and Glycotechology Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Strategy toward the synthesis of glycoconjugate from plant and bacteria2018

    • Author(s)
      Ishiwata, A.
    • Organizer
      Academia Sinica
    • Related Report
      2018 Annual Research Report
  • [Presentation] ビフィズス菌由来 beta-アラビノフラノシダーゼの基質及び阻害剤の立体選択的合成研究2018

    • Author(s)
      石渡 明弘、成田 覚、伊藤 幸成
    • Organizer
      GlycoTokyo 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] B. longum JCM1217由来GH127 β-L-アラビノフラノシダーゼ (HypBA1)の機能構造解析2017

    • Author(s)
      丸山 瞬、伊藤 佑、藤田 清貴、石渡 明弘、伊藤 幸成、荒川 孝俊、伏信 進矢
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大(京都府 京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 1,5-結合したβ-L-アラビノフラノシドを有するオリゴアラビノフラノシドの合成と構造解析2017

    • Author(s)
      石渡 明弘、伊藤 幸成
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大(京都府 京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Bifidobacterium longum subsp. longumのII型アラビノガラクタン分解酵素群の解析2017

    • Author(s)
      藤田 清貴、坂本 彩美、嵩下 愛果、東郷 奈未、北原 兼文
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大(京都府 京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Xanthomonas属細菌由来β-L-アラビノフラノシダーゼの結晶構造2017

    • Author(s)
      澤野 孝太, 丸山 瞬, 荒川 孝俊, 中村 正幸, 石渡 明弘, 伊藤 幸成, 藤田 清貴, 伏信 進矢
    • Organizer
      2017年度生命科学系学会合同年次大会 (ConBio2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] β-L-アラビノフラノシドを有する植物由来オリゴアラビノフラノシドの合成と構造解析2017

    • Author(s)
      石渡 明弘、伊藤 幸成
    • Organizer
      日本化学会
    • Place of Presentation
      慶応大学日吉キャンパス(神奈川県 横浜市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] β-1,3-結合に作用するβ-L-アラビノフラノシダ―ゼの機能解析2016

    • Author(s)
      藤田 清貴、角町華子、石渡明弘、伊藤幸成、北原兼文
    • Organizer
      日本応用糖質科学会大会
    • Place of Presentation
      学校法人福山大学宮地茂記念館(広島県 福山市)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] ビフィズス菌が腸内の糖質を分解するための酵素のかたちと動き2016

    • Author(s)
      伏信進矢
    • Organizer
      日本応用糖質科学会大会
    • Place of Presentation
      広島県民文化センターふくやま(広島県 福山市)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 糖タンパク質糖鎖の加水分解酵素に関する研究2015

    • Author(s)
      藤田清貴
    • Organizer
      日本応用糖質科学会平成27年度大会
    • Place of Presentation
      奈良
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] βーアラビノオリゴ糖鎖に作用するβ-L-アラビノフラノシダーゼの機能解析2015

    • Author(s)
      藤田清貴、亀山恭平、下川倫子、伏信進矢、石渡明弘、Sophon Kaeothip、伊藤幸成、北原兼文
    • Organizer
      第34回日本糖質学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-31
    • Related Report
      2015 Annual Research Report
  • [Remarks] Bibliography of Shinya Fushinobu

    • URL

      http://enzyme13.bt.a.u-tokyo.ac.jp/fushi/pub.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 石渡 明弘 Akihiro Ishiwata (Researchmap)

    • URL

      https://researchmap.jp/a-ishiwata

    • Related Report
      2018 Annual Research Report
  • [Remarks] 藤田 清貴 (Kiyotaka Fujita) (Researchmap)

    • URL

      https://researchmap.jp/read0201683

    • Related Report
      2018 Annual Research Report

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Published: 2015-04-16   Modified: 2022-11-04  

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