| Budget Amount *help |
¥42,250,000 (Direct Cost: ¥32,500,000、Indirect Cost: ¥9,750,000)
Fiscal Year 2017: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2016: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2015: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
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| Outline of Final Research Achievements |
The cDNA sequence of IgNAR of bamboo shark (Chiloscyllium punctatum) was obtained and the sequence of the constant region was extracted. We also established a system to amplify the variable region of IgNAR and measure the expression frequency of individual genes.
Antigens (HEL, BSA, MMP-09) were administered several times to the bamboo shark at intervals of one month, and blood was collected one month after administration. Antibody titer was measured by ELISA for BSA and multiple genes with increased expression frequency were identified in conjunction with rise in antibody titer. Expression constructs combining variable and constant regions of shark antibodies were prepared using CHO cells and succeeded in secretory production. From these results, identification of the antigen-specific antibody gene by transcriptome and establishment of its expression system were almost completed.
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